Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis
Abstract Background The immune mechanism was shown to be involved in the development of adenomyosis. The aim of the current study was to evaluate the expression of the immune checkpoints B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis and to explore the effect of mifepristone on the expression of these...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-021-00800-6 |
| _version_ | 1797629870440710144 |
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| author | Xiaoyan Qin Wenjing Sun Chong Wang Mingjiang Li Xingbo Zhao Changzhong Li Hui Zhang |
| author_facet | Xiaoyan Qin Wenjing Sun Chong Wang Mingjiang Li Xingbo Zhao Changzhong Li Hui Zhang |
| author_sort | Xiaoyan Qin |
| collection | DOAJ |
| description | Abstract Background The immune mechanism was shown to be involved in the development of adenomyosis. The aim of the current study was to evaluate the expression of the immune checkpoints B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis and to explore the effect of mifepristone on the expression of these immune checkpoints. Methods The expression of B7-H2, B7-H3, B7-H4 and PD-L2 in normal endometria and adenomyosis patient samples treated with or without mifepristone was determined by immunohistochemistry analysis. Results In adenomyosis patient samples, the expression of B7-H2, B7-H3 and B7-H4 was increased in the eutopic and ectopic endometria compared with normal endometria, both in the proliferative and secretory phases. Moreover, the expression of B7-H2 and B7-H3 was higher in adenomyotic lesions than in the corresponding eutopic endometria, both in the proliferative and secretory phases. The expression of PD-L2 was higher in adenomyotic lesions than in normal endometria in both the proliferative and secretory phases. In the secretory phase but not the proliferative phase, the expression of B7-H4 and PD-L2 in adenomyotic lesions was significantly higher than that in the corresponding eutopic endometria. In normal endometria and eutopic endometria, the expression of B7-H4 was elevated in the proliferative phase compared with that in the secretory phase, while in the ectopic endometria, B7-H4 expression was decreased in the proliferative phase compared with the secretory phase. In addition, the expression of B7-H2, B7-H3, B7-H4 and PD-L2 was significantly decreased in adenomyosis tissues after treatment with mifepristone. Conclusions The expression of the immune checkpoint proteins B7-H2, B7-H3, B7-H4 and PD-L2 is upregulated in adenomyosis tissues and is downregulated with mifepristone treatment. The data suggest that B7 immunomodulatory molecules are involved in the pathophysiology of adenomyosis. |
| first_indexed | 2024-03-11T10:59:51Z |
| format | Article |
| id | doaj.art-1d912b439ebe4b6da66cf9f017a49976 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-03-11T10:59:51Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-1d912b439ebe4b6da66cf9f017a499762023-11-12T12:33:35ZengBMCReproductive Biology and Endocrinology1477-78272021-07-0119111110.1186/s12958-021-00800-6Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosisXiaoyan Qin0Wenjing Sun1Chong Wang2Mingjiang Li3Xingbo Zhao4Changzhong Li5Hui Zhang6Department of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Surgery, Shandong Rongjun General HospitalDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Obstetrics and Gynaecology, Shandong UniversityDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityAbstract Background The immune mechanism was shown to be involved in the development of adenomyosis. The aim of the current study was to evaluate the expression of the immune checkpoints B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis and to explore the effect of mifepristone on the expression of these immune checkpoints. Methods The expression of B7-H2, B7-H3, B7-H4 and PD-L2 in normal endometria and adenomyosis patient samples treated with or without mifepristone was determined by immunohistochemistry analysis. Results In adenomyosis patient samples, the expression of B7-H2, B7-H3 and B7-H4 was increased in the eutopic and ectopic endometria compared with normal endometria, both in the proliferative and secretory phases. Moreover, the expression of B7-H2 and B7-H3 was higher in adenomyotic lesions than in the corresponding eutopic endometria, both in the proliferative and secretory phases. The expression of PD-L2 was higher in adenomyotic lesions than in normal endometria in both the proliferative and secretory phases. In the secretory phase but not the proliferative phase, the expression of B7-H4 and PD-L2 in adenomyotic lesions was significantly higher than that in the corresponding eutopic endometria. In normal endometria and eutopic endometria, the expression of B7-H4 was elevated in the proliferative phase compared with that in the secretory phase, while in the ectopic endometria, B7-H4 expression was decreased in the proliferative phase compared with the secretory phase. In addition, the expression of B7-H2, B7-H3, B7-H4 and PD-L2 was significantly decreased in adenomyosis tissues after treatment with mifepristone. Conclusions The expression of the immune checkpoint proteins B7-H2, B7-H3, B7-H4 and PD-L2 is upregulated in adenomyosis tissues and is downregulated with mifepristone treatment. The data suggest that B7 immunomodulatory molecules are involved in the pathophysiology of adenomyosis.https://doi.org/10.1186/s12958-021-00800-6B7-H2B7-H3B7-H4PD-L2MifepristoneAdenomyosis |
| spellingShingle | Xiaoyan Qin Wenjing Sun Chong Wang Mingjiang Li Xingbo Zhao Changzhong Li Hui Zhang Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis Reproductive Biology and Endocrinology B7-H2 B7-H3 B7-H4 PD-L2 Mifepristone Adenomyosis |
| title | Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis |
| title_full | Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis |
| title_fullStr | Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis |
| title_full_unstemmed | Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis |
| title_short | Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis |
| title_sort | mifepristone inhibited the expression of b7 h2 b7 h3 b7 h4 and pd l2 in adenomyosis |
| topic | B7-H2 B7-H3 B7-H4 PD-L2 Mifepristone Adenomyosis |
| url | https://doi.org/10.1186/s12958-021-00800-6 |
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