Different response of embryos originating from control and obese mice to insulin in vitro
The aim of the present work was to investigate the impact of maternal obesity on DNA methylation in ovulated oocytes, and to compare the response of in vitro-developing preimplantation embryos originating from control and obese mice to insulin. An intergenerational, diet-induced obesity model was us...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2020-11-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/67/1/67_2020-096/_pdf/-char/en |
| _version_ | 1797615499141447680 |
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| author | Martina KŠIŇANOVÁ Veronika KOVAŘÍKOVÁ Zuzana ŠEFČÍKOVÁ Alexandra ŠPIRKOVÁ Štefan ČIKOŠ Jozef PISKO Dušan FABIAN |
| author_facet | Martina KŠIŇANOVÁ Veronika KOVAŘÍKOVÁ Zuzana ŠEFČÍKOVÁ Alexandra ŠPIRKOVÁ Štefan ČIKOŠ Jozef PISKO Dušan FABIAN |
| author_sort | Martina KŠIŇANOVÁ |
| collection | DOAJ |
| description | The aim of the present work was to investigate the impact of maternal obesity on DNA methylation in ovulated oocytes, and to compare the response of in vitro-developing preimplantation embryos originating from control and obese mice to insulin. An intergenerational, diet-induced obesity model was used to produce outbred mice with an increased body weight and body fat. Two-cell and eight-cell embryos recovered from obese and control mice were cultured in a medium supplemented with 1 or 10 ng/ml insulin until blastocyst formation. In the derived blastocysts, cell proliferation, differentiation, and death rates were determined. The results of immunochemical visualization of 5-methylcytosine indicated a slightly higher DNA methylation in ovulated metaphase II oocytes recovered from obese females; however, the difference between groups did not reach statistical significance. Expanded blastocysts developed from embryos provided by control dams showed increased mean cell numbers (two and eight-cell embryos exposed to 10 ng/ml), an increased inner-cell-mass/trophectoderm ratio (two-cell embryos exposed to 1 ng/ml and eight-cell embryos exposed to 10 ng/ml), and a reduced level of apoptosis (two and eight-cell embryos exposed to 10 ng/ml). In contrast, embryos originating from obese mice were significantly less sensitive to insulin; indeed, no difference was recorded in any tested variable between the embryos exposed to insulin and those cultured in insulin-free medium. Real-time RT-PCR analysis showed a significant increase in the amount of insulin receptor transcripts in blastocysts recovered from obese dams. These results suggest that maternal obesity might modulate the mitogenic and antiapoptotic responses of preimplantation embryos to insulin. |
| first_indexed | 2024-03-11T07:27:22Z |
| format | Article |
| id | doaj.art-1d95304863784fddb63fd54245f6e197 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:27:22Z |
| publishDate | 2020-11-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-1d95304863784fddb63fd54245f6e1972023-11-17T07:29:47ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002020-11-01671253410.1262/jrd.2020-096jrdDifferent response of embryos originating from control and obese mice to insulin in vitroMartina KŠIŇANOVÁ0Veronika KOVAŘÍKOVÁ1Zuzana ŠEFČÍKOVÁ2Alexandra ŠPIRKOVÁ3Štefan ČIKOŠ4Jozef PISKO5Dušan FABIAN6Institute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicInstitute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, 040 01 Košice, Slovak RepublicThe aim of the present work was to investigate the impact of maternal obesity on DNA methylation in ovulated oocytes, and to compare the response of in vitro-developing preimplantation embryos originating from control and obese mice to insulin. An intergenerational, diet-induced obesity model was used to produce outbred mice with an increased body weight and body fat. Two-cell and eight-cell embryos recovered from obese and control mice were cultured in a medium supplemented with 1 or 10 ng/ml insulin until blastocyst formation. In the derived blastocysts, cell proliferation, differentiation, and death rates were determined. The results of immunochemical visualization of 5-methylcytosine indicated a slightly higher DNA methylation in ovulated metaphase II oocytes recovered from obese females; however, the difference between groups did not reach statistical significance. Expanded blastocysts developed from embryos provided by control dams showed increased mean cell numbers (two and eight-cell embryos exposed to 10 ng/ml), an increased inner-cell-mass/trophectoderm ratio (two-cell embryos exposed to 1 ng/ml and eight-cell embryos exposed to 10 ng/ml), and a reduced level of apoptosis (two and eight-cell embryos exposed to 10 ng/ml). In contrast, embryos originating from obese mice were significantly less sensitive to insulin; indeed, no difference was recorded in any tested variable between the embryos exposed to insulin and those cultured in insulin-free medium. Real-time RT-PCR analysis showed a significant increase in the amount of insulin receptor transcripts in blastocysts recovered from obese dams. These results suggest that maternal obesity might modulate the mitogenic and antiapoptotic responses of preimplantation embryos to insulin.https://www.jstage.jst.go.jp/article/jrd/67/1/67_2020-096/_pdf/-char/enapoptosisdna methylationinsulinmaternal obesitypreimplantation embryo |
| spellingShingle | Martina KŠIŇANOVÁ Veronika KOVAŘÍKOVÁ Zuzana ŠEFČÍKOVÁ Alexandra ŠPIRKOVÁ Štefan ČIKOŠ Jozef PISKO Dušan FABIAN Different response of embryos originating from control and obese mice to insulin in vitro The Journal of Reproduction and Development apoptosis dna methylation insulin maternal obesity preimplantation embryo |
| title | Different response of embryos originating from control and obese mice to insulin in vitro |
| title_full | Different response of embryos originating from control and obese mice to insulin in vitro |
| title_fullStr | Different response of embryos originating from control and obese mice to insulin in vitro |
| title_full_unstemmed | Different response of embryos originating from control and obese mice to insulin in vitro |
| title_short | Different response of embryos originating from control and obese mice to insulin in vitro |
| title_sort | different response of embryos originating from control and obese mice to insulin in vitro |
| topic | apoptosis dna methylation insulin maternal obesity preimplantation embryo |
| url | https://www.jstage.jst.go.jp/article/jrd/67/1/67_2020-096/_pdf/-char/en |
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