Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation
Background/Aims Chronic liver disease leads to liver fibrosis, and although the liver does have a certain regenerative capacity, this disease is associated with dysfunction of the liver vessels. C-reactive protein (CRP) is produced in the liver and circulated from there for metabolism. CRP was recen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Association for the Study of the Liver
2016-09-01
|
| Series: | Clinical and Molecular Hepatology |
| Subjects: | |
| Online Access: | http://e-cmh.org/upload/pdf/cmh-2016-0032.pdf |
| _version_ | 1819230203936768000 |
|---|---|
| author | Ji Hye Jun Jong Ho Choi Si Hyun Bae Seh Hoon Oh Gi Jin Kim |
| author_facet | Ji Hye Jun Jong Ho Choi Si Hyun Bae Seh Hoon Oh Gi Jin Kim |
| author_sort | Ji Hye Jun |
| collection | DOAJ |
| description | Background/Aims Chronic liver disease leads to liver fibrosis, and although the liver does have a certain regenerative capacity, this disease is associated with dysfunction of the liver vessels. C-reactive protein (CRP) is produced in the liver and circulated from there for metabolism. CRP was recently shown to inhibit angiogenesis by inducing endothelial cell dysfunction. The objective of this study was to determine the effect of CRP levels on angiogenesis in a rat model of liver dysfunction induced by bile duct ligation (BDL). Methods The diameter of the hepatic vein was analyzed in rat liver tissues using hematoxylin and eosin (H&E) staining. The expression levels of angiogenic factors, albumin, and CRP were analyzed by real-time PCR and Western blotting. A tube formation assay was performed to confirm the effect of CRP on angiogenesis in human umbilical vein endothelial cells (HUVECs) treated with lithocholic acid (LCA) and siRNA-CRP. Results The diameter of the hepatic portal vein increased significantly with the progression of cirrhosis. The expression levels of angiogenic factors were increased in the cirrhotic liver. In contrast, the expression levels of albumin and CRP were significantly lower in the liver tissue obtained from the BDL rat model than in the normal liver. The CRP level was correlated with the expression of albumin in hepatocytes treated with LCA and siRNA-CRP. Tube formation was significantly decreased in HUVECs when they were treated with LCA or a combination of LCA and siRNA-CRP. Conclusion CRP seems to be involved in the abnormal formation of vessels in hepatic disease, and so it could be a useful diagnostic marker for hepatic disease. |
| first_indexed | 2024-12-23T11:25:21Z |
| format | Article |
| id | doaj.art-1d9581547afe42cdb4b0ae7033d6bc5f |
| institution | Directory Open Access Journal |
| issn | 2287-2728 2287-285X |
| language | English |
| last_indexed | 2024-12-23T11:25:21Z |
| publishDate | 2016-09-01 |
| publisher | Korean Association for the Study of the Liver |
| record_format | Article |
| series | Clinical and Molecular Hepatology |
| spelling | doaj.art-1d9581547afe42cdb4b0ae7033d6bc5f2022-12-21T17:48:57ZengKorean Association for the Study of the LiverClinical and Molecular Hepatology2287-27282287-285X2016-09-0122337238110.3350/cmh.2016.00321316Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligationJi Hye Jun0Jong Ho Choi1Si Hyun Bae2Seh Hoon Oh3Gi Jin Kim4 Department of Biomedical Science, CHA University, Seongnam, Korea Department of Biomedical Science, CHA University, Seongnam, Korea Department of Internal Medicine, Catholic University Medical College, Seoul, Korea Department of Pathology, Immunology and Laboratory Medicine, University of Florida, College of Medicine, Gainsville, FL, USA Department of Biomedical Science, CHA University, Seongnam, KoreaBackground/Aims Chronic liver disease leads to liver fibrosis, and although the liver does have a certain regenerative capacity, this disease is associated with dysfunction of the liver vessels. C-reactive protein (CRP) is produced in the liver and circulated from there for metabolism. CRP was recently shown to inhibit angiogenesis by inducing endothelial cell dysfunction. The objective of this study was to determine the effect of CRP levels on angiogenesis in a rat model of liver dysfunction induced by bile duct ligation (BDL). Methods The diameter of the hepatic vein was analyzed in rat liver tissues using hematoxylin and eosin (H&E) staining. The expression levels of angiogenic factors, albumin, and CRP were analyzed by real-time PCR and Western blotting. A tube formation assay was performed to confirm the effect of CRP on angiogenesis in human umbilical vein endothelial cells (HUVECs) treated with lithocholic acid (LCA) and siRNA-CRP. Results The diameter of the hepatic portal vein increased significantly with the progression of cirrhosis. The expression levels of angiogenic factors were increased in the cirrhotic liver. In contrast, the expression levels of albumin and CRP were significantly lower in the liver tissue obtained from the BDL rat model than in the normal liver. The CRP level was correlated with the expression of albumin in hepatocytes treated with LCA and siRNA-CRP. Tube formation was significantly decreased in HUVECs when they were treated with LCA or a combination of LCA and siRNA-CRP. Conclusion CRP seems to be involved in the abnormal formation of vessels in hepatic disease, and so it could be a useful diagnostic marker for hepatic disease.http://e-cmh.org/upload/pdf/cmh-2016-0032.pdfC-reactive proteinBile duct ligationPrimary biliary cirrhosisHepatic disease |
| spellingShingle | Ji Hye Jun Jong Ho Choi Si Hyun Bae Seh Hoon Oh Gi Jin Kim Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation Clinical and Molecular Hepatology C-reactive protein Bile duct ligation Primary biliary cirrhosis Hepatic disease |
| title | Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| title_full | Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| title_fullStr | Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| title_full_unstemmed | Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| title_short | Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| title_sort | decreased c reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation |
| topic | C-reactive protein Bile duct ligation Primary biliary cirrhosis Hepatic disease |
| url | http://e-cmh.org/upload/pdf/cmh-2016-0032.pdf |
| work_keys_str_mv | AT jihyejun decreasedcreactiveproteininducesabnormalvascularstructureinaratmodelofliverdysfunctioninducedbybileductligation AT jonghochoi decreasedcreactiveproteininducesabnormalvascularstructureinaratmodelofliverdysfunctioninducedbybileductligation AT sihyunbae decreasedcreactiveproteininducesabnormalvascularstructureinaratmodelofliverdysfunctioninducedbybileductligation AT sehhoonoh decreasedcreactiveproteininducesabnormalvascularstructureinaratmodelofliverdysfunctioninducedbybileductligation AT gijinkim decreasedcreactiveproteininducesabnormalvascularstructureinaratmodelofliverdysfunctioninducedbybileductligation |