The haemoculture of Trypanosoma minasense chagas, 1908
Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1996-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019 |
| _version_ | 1827842503656603648 |
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| author | Mariangela Ziccardi Ricardo Lourenço-de-Oliveira Ricardo Nogueira |
| author_facet | Mariangela Ziccardi Ricardo Lourenço-de-Oliveira Ricardo Nogueira |
| author_sort | Mariangela Ziccardi |
| collection | DOAJ |
| description | Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated. |
| first_indexed | 2024-03-12T08:10:28Z |
| format | Article |
| id | doaj.art-1d97084b0ba74062ae2437eebcc38d2b |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:10:28Z |
| publishDate | 1996-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1d97084b0ba74062ae2437eebcc38d2b2023-09-02T19:07:06ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601996-08-0191450150510.1590/S0074-02761996000400019The haemoculture of Trypanosoma minasense chagas, 1908Mariangela ZiccardiRicardo Lourenço-de-OliveiraRicardo NogueiraTrypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019Trypanosoma minasenseneotropical primatestrypanosomesmarmosetstrypanosomatidae flagellatescultureBrazil |
| spellingShingle | Mariangela Ziccardi Ricardo Lourenço-de-Oliveira Ricardo Nogueira The haemoculture of Trypanosoma minasense chagas, 1908 Memorias do Instituto Oswaldo Cruz Trypanosoma minasense neotropical primates trypanosomes marmosets trypanosomatidae flagellates culture Brazil |
| title | The haemoculture of Trypanosoma minasense chagas, 1908 |
| title_full | The haemoculture of Trypanosoma minasense chagas, 1908 |
| title_fullStr | The haemoculture of Trypanosoma minasense chagas, 1908 |
| title_full_unstemmed | The haemoculture of Trypanosoma minasense chagas, 1908 |
| title_short | The haemoculture of Trypanosoma minasense chagas, 1908 |
| title_sort | haemoculture of trypanosoma minasense chagas 1908 |
| topic | Trypanosoma minasense neotropical primates trypanosomes marmosets trypanosomatidae flagellates culture Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019 |
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