A non-invasive method of quantifying pancreatic volume in mice using micro-MRI.
In experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely underg...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3958493?pdf=render |
| _version_ | 1819135489205075968 |
|---|---|
| author | Jose L Paredes Abrahim I Orabi Taimur Ahmad Iman Benbourenane Kimimasa Tobita Sameh Tadros Kyongtae T Bae Sohail Z Husain |
| author_facet | Jose L Paredes Abrahim I Orabi Taimur Ahmad Iman Benbourenane Kimimasa Tobita Sameh Tadros Kyongtae T Bae Sohail Z Husain |
| author_sort | Jose L Paredes |
| collection | DOAJ |
| description | In experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely undergo non-invasive cross-sectional imaging of the pancreas using magnetic resonance imaging (MRI) or computed tomography (CT). The aim of the current study was to develop a thin-sliced, optimized sequence protocol using a high field MRI to accurately calculate pancreatic volumes in the most common experimental animal, the mouse. Using a 7 Telsa Bruker micro-MRI system, we performed abdominal imaging in whole-fixed mice in three standard planes: axial, sagittal, and coronal. The contour of the pancreas was traced using Vitrea software and then transformed into a 3-dimensional (3D) reconstruction, from which volumetric measurements were calculated. Images were optimized using heart perfusion-fixation, T1 sequence analysis, and 0.2 to 0.4 mm thick slices. As proof of principle, increases in pancreatic volume among mice of different ages correlated tightly with increasing body weight. In summary, this is the first study to measure pancreatic volumes in mice, using a high field 7 Tesla micro-MRI and a thin-sliced, optimized sequence protocol. We anticipate that micro-MRI will improve the ability to non-invasively quantify changes in pancreatic size and will dramatically reduce the number of animals required to serially assess pancreatic growth and recovery. |
| first_indexed | 2024-12-22T10:19:54Z |
| format | Article |
| id | doaj.art-1d9921000f32430cb12a2c71b28424e8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T10:19:54Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1d9921000f32430cb12a2c71b28424e82022-12-21T18:29:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9226310.1371/journal.pone.0092263A non-invasive method of quantifying pancreatic volume in mice using micro-MRI.Jose L ParedesAbrahim I OrabiTaimur AhmadIman BenbourenaneKimimasa TobitaSameh TadrosKyongtae T BaeSohail Z HusainIn experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely undergo non-invasive cross-sectional imaging of the pancreas using magnetic resonance imaging (MRI) or computed tomography (CT). The aim of the current study was to develop a thin-sliced, optimized sequence protocol using a high field MRI to accurately calculate pancreatic volumes in the most common experimental animal, the mouse. Using a 7 Telsa Bruker micro-MRI system, we performed abdominal imaging in whole-fixed mice in three standard planes: axial, sagittal, and coronal. The contour of the pancreas was traced using Vitrea software and then transformed into a 3-dimensional (3D) reconstruction, from which volumetric measurements were calculated. Images were optimized using heart perfusion-fixation, T1 sequence analysis, and 0.2 to 0.4 mm thick slices. As proof of principle, increases in pancreatic volume among mice of different ages correlated tightly with increasing body weight. In summary, this is the first study to measure pancreatic volumes in mice, using a high field 7 Tesla micro-MRI and a thin-sliced, optimized sequence protocol. We anticipate that micro-MRI will improve the ability to non-invasively quantify changes in pancreatic size and will dramatically reduce the number of animals required to serially assess pancreatic growth and recovery.http://europepmc.org/articles/PMC3958493?pdf=render |
| spellingShingle | Jose L Paredes Abrahim I Orabi Taimur Ahmad Iman Benbourenane Kimimasa Tobita Sameh Tadros Kyongtae T Bae Sohail Z Husain A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. PLoS ONE |
| title | A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. |
| title_full | A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. |
| title_fullStr | A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. |
| title_full_unstemmed | A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. |
| title_short | A non-invasive method of quantifying pancreatic volume in mice using micro-MRI. |
| title_sort | non invasive method of quantifying pancreatic volume in mice using micro mri |
| url | http://europepmc.org/articles/PMC3958493?pdf=render |
| work_keys_str_mv | AT joselparedes anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT abrahimiorabi anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT taimurahmad anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT imanbenbourenane anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT kimimasatobita anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT samehtadros anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT kyongtaetbae anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT sohailzhusain anoninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT joselparedes noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT abrahimiorabi noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT taimurahmad noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT imanbenbourenane noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT kimimasatobita noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT samehtadros noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT kyongtaetbae noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri AT sohailzhusain noninvasivemethodofquantifyingpancreaticvolumeinmiceusingmicromri |