An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode
Procalcitonin (PCT) is considered a sepsis and infection biomarker. Herein, an interdigitated electrochemical immunosensor for the determination of PCT has been developed. The interdigitated electrode was made of the laser-engraved graphene electrode decorated with gold (LEGE/Au<sub>nano</s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/771 |
| _version_ | 1797474827210063872 |
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| author | Mahmoud Amouzadeh Tabrizi Pablo Acedo |
| author_facet | Mahmoud Amouzadeh Tabrizi Pablo Acedo |
| author_sort | Mahmoud Amouzadeh Tabrizi |
| collection | DOAJ |
| description | Procalcitonin (PCT) is considered a sepsis and infection biomarker. Herein, an interdigitated electrochemical immunosensor for the determination of PCT has been developed. The interdigitated electrode was made of the laser-engraved graphene electrode decorated with gold (LEGE/Au<sub>nano</sub>). The scanning electron microscopy indicated the LEGE/Au<sub>nano</sub> has been fabricated successfully. After that, the anti-PTC antibodies were immobilized on the surface of the electrode by using 3-mercaptopropionic acid. The electrochemical performance of the fabricated immunosensor was studied using electrochemical impedance spectroscopy (EIS). The EIS method was used for the determination of PCT in the concentration range of 2.5–800 pg/mL with a limit of detection of 0.36 pg/mL. The effect of several interfering agents such as the C reactive protein (CRP), immunoglobulin G (IgG), and human serum albumin (HSA) was also studied. The fabricated immunosensor had a good selectivity to the PCT. The stability of the immunosensor was also studied for 1 month. The relative standard deviation (RSD) was obtained to be 5.2%. |
| first_indexed | 2024-03-09T20:35:39Z |
| format | Article |
| id | doaj.art-1d9b83150d2548e099d270c857e6c215 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:35:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1d9b83150d2548e099d270c857e6c2152023-11-23T23:10:23ZengMDPI AGBiosensors2079-63742022-09-01121077110.3390/bios12100771An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated ElectrodeMahmoud Amouzadeh Tabrizi0Pablo Acedo1Electronic Technology Department, Universidad Carlos III de Madrid, 28911 Leganés, SpainElectronic Technology Department, Universidad Carlos III de Madrid, 28911 Leganés, SpainProcalcitonin (PCT) is considered a sepsis and infection biomarker. Herein, an interdigitated electrochemical immunosensor for the determination of PCT has been developed. The interdigitated electrode was made of the laser-engraved graphene electrode decorated with gold (LEGE/Au<sub>nano</sub>). The scanning electron microscopy indicated the LEGE/Au<sub>nano</sub> has been fabricated successfully. After that, the anti-PTC antibodies were immobilized on the surface of the electrode by using 3-mercaptopropionic acid. The electrochemical performance of the fabricated immunosensor was studied using electrochemical impedance spectroscopy (EIS). The EIS method was used for the determination of PCT in the concentration range of 2.5–800 pg/mL with a limit of detection of 0.36 pg/mL. The effect of several interfering agents such as the C reactive protein (CRP), immunoglobulin G (IgG), and human serum albumin (HSA) was also studied. The fabricated immunosensor had a good selectivity to the PCT. The stability of the immunosensor was also studied for 1 month. The relative standard deviation (RSD) was obtained to be 5.2%.https://www.mdpi.com/2079-6374/12/10/771interdigitated electrodelaser engraved grapheneimmunosensorelectrochemistryprocalcitonin |
| spellingShingle | Mahmoud Amouzadeh Tabrizi Pablo Acedo An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode Biosensors interdigitated electrode laser engraved graphene immunosensor electrochemistry procalcitonin |
| title | An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode |
| title_full | An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode |
| title_fullStr | An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode |
| title_full_unstemmed | An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode |
| title_short | An Electrochemical Immunosensor for the Determination of Procalcitonin Using the Gold-Graphene Interdigitated Electrode |
| title_sort | electrochemical immunosensor for the determination of procalcitonin using the gold graphene interdigitated electrode |
| topic | interdigitated electrode laser engraved graphene immunosensor electrochemistry procalcitonin |
| url | https://www.mdpi.com/2079-6374/12/10/771 |
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