Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)

ABSTRACT: Gray mould, caused by the fungus Botrytis cinerea Pers., is a disease that largely affects the crops of Rubus glaucus Benth. (Castilla blackberry) in Colombia. In spite of the economic losses that it causes in the production of Castilla blackberry at national level, a standardized method t...

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Main Authors: Sarah Muñoz Arias, Gloria Edith Guerrero Álvarez, Paula Andrea González Patiño
Format: Article
Language:English
Published: Universidade Federal de Santa Maria
Series:Ciência Rural
Subjects:
Online Access:http://www.scielo.br/pdf/cr/v50n11/1678-4596-cr-50-11-e20190859.pdf
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author Sarah Muñoz Arias
Gloria Edith Guerrero Álvarez
Paula Andrea González Patiño
author_facet Sarah Muñoz Arias
Gloria Edith Guerrero Álvarez
Paula Andrea González Patiño
author_sort Sarah Muñoz Arias
collection DOAJ
description ABSTRACT: Gray mould, caused by the fungus Botrytis cinerea Pers., is a disease that largely affects the crops of Rubus glaucus Benth. (Castilla blackberry) in Colombia. In spite of the economic losses that it causes in the production of Castilla blackberry at national level, a standardized method to quantify the disease severity caused by this fungus in the fruits has not yet been reported. In the present work, a diagrammatic scale was prepared to assess gray mould severity in thornless fruits of R. glaucus. The proposed scale showed the levels of 5, 10, 20, 40, 60, 80, and 100%. Photographic images were obtained for each of these levels, which were processed using the ImageJ software. The scale was validated by 13 evaluators who assessed affected fruits with and without the scale. The precision and accuracy of each evaluator was determined by simple linear regression between actual and estimated severity. The evaluators showed better precision, accuracy, and reproducibility in the assessments performed with the scale. The proposed scale is appropriate to estimate the severity of gray mould in R. glaucus fruits.
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spelling doaj.art-1d9fc3df92a74d6b8064983bf2adde8b2022-12-21T18:12:21ZengUniversidade Federal de Santa MariaCiência Rural1678-459610.1590/0103-8478cr20190859Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)Sarah Muñoz AriasGloria Edith Guerrero ÁlvarezPaula Andrea González PatiñoABSTRACT: Gray mould, caused by the fungus Botrytis cinerea Pers., is a disease that largely affects the crops of Rubus glaucus Benth. (Castilla blackberry) in Colombia. In spite of the economic losses that it causes in the production of Castilla blackberry at national level, a standardized method to quantify the disease severity caused by this fungus in the fruits has not yet been reported. In the present work, a diagrammatic scale was prepared to assess gray mould severity in thornless fruits of R. glaucus. The proposed scale showed the levels of 5, 10, 20, 40, 60, 80, and 100%. Photographic images were obtained for each of these levels, which were processed using the ImageJ software. The scale was validated by 13 evaluators who assessed affected fruits with and without the scale. The precision and accuracy of each evaluator was determined by simple linear regression between actual and estimated severity. The evaluators showed better precision, accuracy, and reproducibility in the assessments performed with the scale. The proposed scale is appropriate to estimate the severity of gray mould in R. glaucus fruits.http://www.scielo.br/pdf/cr/v50n11/1678-4596-cr-50-11-e20190859.pdfBotrytis cinerea Pers.measuring diseasepathometryRubus glaucus Benth.
spellingShingle Sarah Muñoz Arias
Gloria Edith Guerrero Álvarez
Paula Andrea González Patiño
Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
Ciência Rural
Botrytis cinerea Pers.
measuring disease
pathometry
Rubus glaucus Benth.
title Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
title_full Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
title_fullStr Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
title_full_unstemmed Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
title_short Diagrammatic scale for measuring severity of gray mould in thornless Castilla blackberry (Rubus glaucus Benth)
title_sort diagrammatic scale for measuring severity of gray mould in thornless castilla blackberry rubus glaucus benth
topic Botrytis cinerea Pers.
measuring disease
pathometry
Rubus glaucus Benth.
url http://www.scielo.br/pdf/cr/v50n11/1678-4596-cr-50-11-e20190859.pdf
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AT paulaandreagonzalezpatino diagrammaticscaleformeasuringseverityofgraymouldinthornlesscastillablackberryrubusglaucusbenth