Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses
Lung metastasis is a leading cause of cancer-related deaths. Here, we show that intranasal delivery of our engineered CpG-coated tumor antigen (Tag)-encapsulated nanoparticles (NPs)—nasal nano-vaccine—significantly reduced lung colonization by intravenous challenge of an extra-pulmonary tumor. Prote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/2/445 |
| _version_ | 1827755900377497600 |
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| author | Michael Donkor Jamie Choe Danielle Marie Reid Byron Quinn Mark Pulse Amalendu Ranjan Pankaj Chaudhary Harlan P. Jones |
| author_facet | Michael Donkor Jamie Choe Danielle Marie Reid Byron Quinn Mark Pulse Amalendu Ranjan Pankaj Chaudhary Harlan P. Jones |
| author_sort | Michael Donkor |
| collection | DOAJ |
| description | Lung metastasis is a leading cause of cancer-related deaths. Here, we show that intranasal delivery of our engineered CpG-coated tumor antigen (Tag)-encapsulated nanoparticles (NPs)—nasal nano-vaccine—significantly reduced lung colonization by intravenous challenge of an extra-pulmonary tumor. Protection against tumor-cell lung colonization was linked to the induction of localized mucosal-associated effector and resident memory T cells as well as increased bronchiolar alveolar lavage-fluid IgA and serum IgG antibody responses. The nasal nano-vaccine-induced T-cell-mediated antitumor mucosal immune response was shown to increase tumor-specific production of IFN-γ and granzyme B by lung-derived CD8<sup>+</sup> T cells. These findings demonstrate that our engineered nasal nano-vaccine has the potential to be used as a prophylactic approach prior to the seeding of tumors in the lungs, and thereby prevent overt lung metastases from existing extra pulmonary tumors. |
| first_indexed | 2024-03-11T08:16:35Z |
| format | Article |
| id | doaj.art-1da2052990a341b092d4051d64195cdb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:16:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-1da2052990a341b092d4051d64195cdb2023-11-16T22:40:01ZengMDPI AGPharmaceutics1999-49232023-01-0115244510.3390/pharmaceutics15020445Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune ResponsesMichael Donkor0Jamie Choe1Danielle Marie Reid2Byron Quinn3Mark Pulse4Amalendu Ranjan5Pankaj Chaudhary6Harlan P. Jones7Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Biology, Langston University, Langston, OK 73050, USADepartment of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USADepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USALung metastasis is a leading cause of cancer-related deaths. Here, we show that intranasal delivery of our engineered CpG-coated tumor antigen (Tag)-encapsulated nanoparticles (NPs)—nasal nano-vaccine—significantly reduced lung colonization by intravenous challenge of an extra-pulmonary tumor. Protection against tumor-cell lung colonization was linked to the induction of localized mucosal-associated effector and resident memory T cells as well as increased bronchiolar alveolar lavage-fluid IgA and serum IgG antibody responses. The nasal nano-vaccine-induced T-cell-mediated antitumor mucosal immune response was shown to increase tumor-specific production of IFN-γ and granzyme B by lung-derived CD8<sup>+</sup> T cells. These findings demonstrate that our engineered nasal nano-vaccine has the potential to be used as a prophylactic approach prior to the seeding of tumors in the lungs, and thereby prevent overt lung metastases from existing extra pulmonary tumors.https://www.mdpi.com/1999-4923/15/2/445breast cancerlung metastasisvaccinationimmune responsenanoparticles |
| spellingShingle | Michael Donkor Jamie Choe Danielle Marie Reid Byron Quinn Mark Pulse Amalendu Ranjan Pankaj Chaudhary Harlan P. Jones Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses Pharmaceutics breast cancer lung metastasis vaccination immune response nanoparticles |
| title | Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses |
| title_full | Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses |
| title_fullStr | Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses |
| title_full_unstemmed | Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses |
| title_short | Nasal Tumor Vaccination Protects against Lung Tumor Development by Induction of Resident Effector and Memory Anti-Tumor Immune Responses |
| title_sort | nasal tumor vaccination protects against lung tumor development by induction of resident effector and memory anti tumor immune responses |
| topic | breast cancer lung metastasis vaccination immune response nanoparticles |
| url | https://www.mdpi.com/1999-4923/15/2/445 |
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