Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma
The infiltration of immune cells can significantly affect the prognosis and immune therapy of patients with cervical squamous cell carcinoma (CSCC). This study aimed to explore key immune cell-related genes in the tumorigenesis and prognosis of CSCC. The module significantly related to immunity was...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-08-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2023.2254239 |
| _version_ | 1797633695372279808 |
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| author | Chunxiao Yan Yanyan Ma Junyan Li Xuejun Chen Jiong Ma |
| author_facet | Chunxiao Yan Yanyan Ma Junyan Li Xuejun Chen Jiong Ma |
| author_sort | Chunxiao Yan |
| collection | DOAJ |
| description | The infiltration of immune cells can significantly affect the prognosis and immune therapy of patients with cervical squamous cell carcinoma (CSCC). This study aimed to explore key immune cell-related genes in the tumorigenesis and prognosis of CSCC. The module significantly related to immunity was screened by weighted gene co-expression network analysis (WGCNA) and ESTIMATE analysis, followed by correlation analysis with clinical traits. Key candidate genes were intersected with the protein–protein interaction (PPI) network genes for immune-related genes. The relationship between immune cell infiltration and key genes was analyzed. Tumor immune dysfunction and exclusion (TIDE) and immunophenoscore (IPS) predicted the response to immunotherapy in CSCC patients. Clinically, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were manipulated for analyzing the changes in mRNA and protein expression of key genes in cancer. Western blot was conducted to assess the correlation between key genes and immune infiltration. The brown module was notably associated with the immune microenvironment of CSCC, from which three immune-related key genes (TYROBP, CCL5, and HLA-DRA) were obtained. High expression of these genes was significantly positively associated with the infiltration abundance of T cells, B cells, and other immune cells. High expression levels of three key genes were confirmed in para-cancer tissue and correlated with the abundance of immune cells. The high-expression group of key genes was more sensitive to immunotherapy. We provide a theoretical basis for searching for potential targets for effective treatment and diagnosis of CSCC and provide new ideas for developing novel immunotherapy strategies. |
| first_indexed | 2024-03-11T11:57:29Z |
| format | Article |
| id | doaj.art-1da2cba22e064adb9c859b9953fa9bbb |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T11:57:29Z |
| publishDate | 2023-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-1da2cba22e064adb9c859b9953fa9bbb2023-11-08T11:55:20ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2023-08-0119210.1080/21645515.2023.22542392254239Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinomaChunxiao Yan0Yanyan Ma1Junyan Li2Xuejun Chen3Jiong Ma4The Second Affiliated Hospital of Zhejiang UniversityThe Second Affiliated Hospital of Zhejiang UniversityThe Second Affiliated Hospital of Zhejiang UniversityThe Second Affiliated Hospital of Zhejiang UniversityThe Second Affiliated Hospital of Zhejiang UniversityThe infiltration of immune cells can significantly affect the prognosis and immune therapy of patients with cervical squamous cell carcinoma (CSCC). This study aimed to explore key immune cell-related genes in the tumorigenesis and prognosis of CSCC. The module significantly related to immunity was screened by weighted gene co-expression network analysis (WGCNA) and ESTIMATE analysis, followed by correlation analysis with clinical traits. Key candidate genes were intersected with the protein–protein interaction (PPI) network genes for immune-related genes. The relationship between immune cell infiltration and key genes was analyzed. Tumor immune dysfunction and exclusion (TIDE) and immunophenoscore (IPS) predicted the response to immunotherapy in CSCC patients. Clinically, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were manipulated for analyzing the changes in mRNA and protein expression of key genes in cancer. Western blot was conducted to assess the correlation between key genes and immune infiltration. The brown module was notably associated with the immune microenvironment of CSCC, from which three immune-related key genes (TYROBP, CCL5, and HLA-DRA) were obtained. High expression of these genes was significantly positively associated with the infiltration abundance of T cells, B cells, and other immune cells. High expression levels of three key genes were confirmed in para-cancer tissue and correlated with the abundance of immune cells. The high-expression group of key genes was more sensitive to immunotherapy. We provide a theoretical basis for searching for potential targets for effective treatment and diagnosis of CSCC and provide new ideas for developing novel immunotherapy strategies.http://dx.doi.org/10.1080/21645515.2023.2254239cervical squamous cell carcinomawgcnaestimateimmunotherapy |
| spellingShingle | Chunxiao Yan Yanyan Ma Junyan Li Xuejun Chen Jiong Ma Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma Human Vaccines & Immunotherapeutics cervical squamous cell carcinoma wgcna estimate immunotherapy |
| title | Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| title_full | Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| title_fullStr | Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| title_full_unstemmed | Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| title_short | Identification of key immune cell-related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| title_sort | identification of key immune cell related genes involved in tumorigenesis and prognosis of cervical squamous cell carcinoma |
| topic | cervical squamous cell carcinoma wgcna estimate immunotherapy |
| url | http://dx.doi.org/10.1080/21645515.2023.2254239 |
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