Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows
The aim of the study was to examine the effect of lameness and energy status on the involution of the uterus and the resumption of ovarian cyclicity in dairy cows. Lame (lameness score of four and the presence of hoof lesions, <i>n</i> = 22) and sound (normal gait and the absence of hoof...
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2023-11-01
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| author | Anastasia Praxitelous Panagiotis D. Katsoulos Angeliki Tsaousioti Christos Brozos Marion Schmicke Constantin M. Boscos Georgios Tsousis |
| author_facet | Anastasia Praxitelous Panagiotis D. Katsoulos Angeliki Tsaousioti Christos Brozos Marion Schmicke Constantin M. Boscos Georgios Tsousis |
| author_sort | Anastasia Praxitelous |
| collection | DOAJ |
| description | The aim of the study was to examine the effect of lameness and energy status on the involution of the uterus and the resumption of ovarian cyclicity in dairy cows. Lame (lameness score of four and the presence of hoof lesions, <i>n</i> = 22) and sound (normal gait and the absence of hoof lesions, <i>n</i> = 25) multiparous cows with healthy puerperium were enrolled simultaneously in the study and were monitored from day 10 antepartum (ap) to day 50 post-partum (pp). Ultrasonography of the cervix, the formerly gravid uterine horn and the ovarian structures was performed on d 8, 11, 14, 23, 30, and 42 pp. Blood sampling for progesterone, β-hydroxybutyrate (BHBA), and non-esterified fatty acids (NEFAs) was used to assess cyclicity and energy status. Lame compared to sound cows had higher NEFA concentrations on day 14 pp (0.54 ± 0.05 vs. 0.37 ± 0.05, respectively, <i>p</i> = 0.005), delayed involution of the cervix and the formerly pregnant uterine horn (<i>p</i> = 0.0003 and <i>p</i> = 0.02, respectively), lower ovulation rates within the experimental period (63.6% vs. 88%, respectively, <i>p</i> = 0.05), and higher rates of atresia or cyst formation on day 50 pp (36.4% vs. 12%, respectively, <i>p</i> = 0.05). Independently of lameness status, cows with high NEFA concentrations had lower ovulation rates within the experimental period (65.5% vs. 94.4%, <i>p</i> = 0.02), lower normal ovarian activity on day 50 pp (58.6% vs. 88.9%, <i>p</i> = 0.03), and higher rates of atresia or cyst formation on day 50 pp (34.5% vs. 5.6%, <i>p</i> = 0.02) compared to cows with optimal NEFA concentrations. Furthermore, an interaction between lameness and increased NEFA concentrations was observed regarding the ovulation rate within the experimental period and the percentage of atresia or cyst formation on day 50 pp. Sound cows with low NEFA levels had the lowest mean cervical diameter compared to cows with lameness (both with elevated and optimal NEFA concentrations, <i>p</i> = 0.009 and <i>p</i> = 0.002, respectively). Conclusively, lameness during puerperium negatively affected ovarian function and uterine involution. These effects were exacerbated (through interaction or cumulation) in relation to elevated NEFA concentrations. |
| first_indexed | 2024-03-09T01:55:26Z |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-09T01:55:26Z |
| publishDate | 2023-11-01 |
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| spelling | doaj.art-1da4a6505efb4c54a0359c68e05662762023-12-08T15:10:33ZengMDPI AGAnimals2076-26152023-11-011323364510.3390/ani13233645Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein CowsAnastasia Praxitelous0Panagiotis D. Katsoulos1Angeliki Tsaousioti2Christos Brozos3Marion Schmicke4Constantin M. Boscos5Georgios Tsousis6Clinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceClinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceClinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceClinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceClinic for Cattle, Veterinary Endocrinology and Laboratory Diagnostic, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyClinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceClinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceThe aim of the study was to examine the effect of lameness and energy status on the involution of the uterus and the resumption of ovarian cyclicity in dairy cows. Lame (lameness score of four and the presence of hoof lesions, <i>n</i> = 22) and sound (normal gait and the absence of hoof lesions, <i>n</i> = 25) multiparous cows with healthy puerperium were enrolled simultaneously in the study and were monitored from day 10 antepartum (ap) to day 50 post-partum (pp). Ultrasonography of the cervix, the formerly gravid uterine horn and the ovarian structures was performed on d 8, 11, 14, 23, 30, and 42 pp. Blood sampling for progesterone, β-hydroxybutyrate (BHBA), and non-esterified fatty acids (NEFAs) was used to assess cyclicity and energy status. Lame compared to sound cows had higher NEFA concentrations on day 14 pp (0.54 ± 0.05 vs. 0.37 ± 0.05, respectively, <i>p</i> = 0.005), delayed involution of the cervix and the formerly pregnant uterine horn (<i>p</i> = 0.0003 and <i>p</i> = 0.02, respectively), lower ovulation rates within the experimental period (63.6% vs. 88%, respectively, <i>p</i> = 0.05), and higher rates of atresia or cyst formation on day 50 pp (36.4% vs. 12%, respectively, <i>p</i> = 0.05). Independently of lameness status, cows with high NEFA concentrations had lower ovulation rates within the experimental period (65.5% vs. 94.4%, <i>p</i> = 0.02), lower normal ovarian activity on day 50 pp (58.6% vs. 88.9%, <i>p</i> = 0.03), and higher rates of atresia or cyst formation on day 50 pp (34.5% vs. 5.6%, <i>p</i> = 0.02) compared to cows with optimal NEFA concentrations. Furthermore, an interaction between lameness and increased NEFA concentrations was observed regarding the ovulation rate within the experimental period and the percentage of atresia or cyst formation on day 50 pp. Sound cows with low NEFA levels had the lowest mean cervical diameter compared to cows with lameness (both with elevated and optimal NEFA concentrations, <i>p</i> = 0.009 and <i>p</i> = 0.002, respectively). Conclusively, lameness during puerperium negatively affected ovarian function and uterine involution. These effects were exacerbated (through interaction or cumulation) in relation to elevated NEFA concentrations.https://www.mdpi.com/2076-2615/13/23/3645lamenessdairy cattleuterine involutionovarian resumptionenergy status |
| spellingShingle | Anastasia Praxitelous Panagiotis D. Katsoulos Angeliki Tsaousioti Christos Brozos Marion Schmicke Constantin M. Boscos Georgios Tsousis Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows Animals lameness dairy cattle uterine involution ovarian resumption energy status |
| title | Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows |
| title_full | Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows |
| title_fullStr | Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows |
| title_full_unstemmed | Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows |
| title_short | Comparison of Uterine Involution and the Resumption of Ovarian Cyclicity between Lame and Sound Holstein Cows |
| title_sort | comparison of uterine involution and the resumption of ovarian cyclicity between lame and sound holstein cows |
| topic | lameness dairy cattle uterine involution ovarian resumption energy status |
| url | https://www.mdpi.com/2076-2615/13/23/3645 |
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