Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids
Testing inbred lines for their combining ability is, due to high numbers of line to line testing needed for determination of hybrid performance, the most limiting factor in the F1 hybrid breeding procedure. We propose a novel method of F1 hybrid breeding that enables evaluation of large number of li...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-09-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01111/full |
| _version_ | 1811201958043713536 |
|---|---|
| author | Katarina Rudolf-Pilih Marko Petkovšek Jernej Jakše Nataša Štajner Jana Murovec Borut Bohanec |
| author_facet | Katarina Rudolf-Pilih Marko Petkovšek Jernej Jakše Nataša Štajner Jana Murovec Borut Bohanec |
| author_sort | Katarina Rudolf-Pilih |
| collection | DOAJ |
| description | Testing inbred lines for their combining ability is, due to high numbers of line to line testing needed for determination of hybrid performance, the most limiting factor in the F1 hybrid breeding procedure. We propose a novel method of F1 hybrid breeding that enables evaluation of large number of line to line crosses for their hybrid performance. Inbred lines (preferably doubled haploid - DH) are produced from heterozygous populations, genotyped and maintained. A group of lines is inter-pollinated randomly and their progeny examined. To identify elite F1 hybrids, these individual plants are selected by their superior phenotypic characteristics. Finally using paternity testing only of selected hybrids, the origin of paternal lines is revealed. To predict the number of F1 offspring needed in relation to the number of inbred lines being inter-pollinated, a mathematical formula was developed. For instance, using this formula for the inter-pollination of 60 distinct lines, the probability of obtaining all descendants of paternal-parent lines in a maternal-parent row represented at least once is achieved with 420 F1 plants in a row (p = 0.95). In a practical experiment with white cabbage, DH lines were produced using microspore culture; plants were grown to maturity and genotyped at eight polymorphic SSR loci. Two groups of lines (36 and 33 lines per group) were inter-pollinated by two methods, either using cage pollination with bumblebees or using open pollination in isolated field. A total of 9,858 F1 plants were planted and based on their phenotypic characteristics 213 were selected as elite phenotypes. 99 of them were genetically diverse and 5 of them were selected as super elite. Selected plants were analysed by the same SSR markers and the paternal origin of selected F1 plants was determined. Out of 213 selected elite plants 48 were reciprocals thus exhibiting power of selection based on single plant. We demonstrate that this new approach to hybrid development is efficient in white cabbage and we propose breeders to test it in various vegetable and crop species. Moreover, some other aspects of the proposed technique need to be tested and verified both for practical and economic criteria. |
| first_indexed | 2024-04-12T02:31:49Z |
| format | Article |
| id | doaj.art-1daa0d3827dc43b1a9cbc16702bb42e5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T02:31:49Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1daa0d3827dc43b1a9cbc16702bb42e52022-12-22T03:51:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-09-011010.3389/fpls.2019.01111478475Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 HybridsKatarina Rudolf-Pilih0Marko Petkovšek1Jernej Jakše2Nataša Štajner3Jana Murovec4Borut Bohanec5Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaFaculty of Mathematics and Physics, University of Ljubljana, Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaTesting inbred lines for their combining ability is, due to high numbers of line to line testing needed for determination of hybrid performance, the most limiting factor in the F1 hybrid breeding procedure. We propose a novel method of F1 hybrid breeding that enables evaluation of large number of line to line crosses for their hybrid performance. Inbred lines (preferably doubled haploid - DH) are produced from heterozygous populations, genotyped and maintained. A group of lines is inter-pollinated randomly and their progeny examined. To identify elite F1 hybrids, these individual plants are selected by their superior phenotypic characteristics. Finally using paternity testing only of selected hybrids, the origin of paternal lines is revealed. To predict the number of F1 offspring needed in relation to the number of inbred lines being inter-pollinated, a mathematical formula was developed. For instance, using this formula for the inter-pollination of 60 distinct lines, the probability of obtaining all descendants of paternal-parent lines in a maternal-parent row represented at least once is achieved with 420 F1 plants in a row (p = 0.95). In a practical experiment with white cabbage, DH lines were produced using microspore culture; plants were grown to maturity and genotyped at eight polymorphic SSR loci. Two groups of lines (36 and 33 lines per group) were inter-pollinated by two methods, either using cage pollination with bumblebees or using open pollination in isolated field. A total of 9,858 F1 plants were planted and based on their phenotypic characteristics 213 were selected as elite phenotypes. 99 of them were genetically diverse and 5 of them were selected as super elite. Selected plants were analysed by the same SSR markers and the paternal origin of selected F1 plants was determined. Out of 213 selected elite plants 48 were reciprocals thus exhibiting power of selection based on single plant. We demonstrate that this new approach to hybrid development is efficient in white cabbage and we propose breeders to test it in various vegetable and crop species. Moreover, some other aspects of the proposed technique need to be tested and verified both for practical and economic criteria.https://www.frontiersin.org/article/10.3389/fpls.2019.01111/fullF1 hybrid breedingdoubled haploidstesting of combining abilitypaternity determinationsimple sequence repeats markers |
| spellingShingle | Katarina Rudolf-Pilih Marko Petkovšek Jernej Jakše Nataša Štajner Jana Murovec Borut Bohanec Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids Frontiers in Plant Science F1 hybrid breeding doubled haploids testing of combining ability paternity determination simple sequence repeats markers |
| title | Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids |
| title_full | Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids |
| title_fullStr | Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids |
| title_full_unstemmed | Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids |
| title_short | Proposal of a New Hybrid Breeding Method Based on Genotyping, Inter-Pollination, Phenotyping and Paternity Testing of Selected Elite F1 Hybrids |
| title_sort | proposal of a new hybrid breeding method based on genotyping inter pollination phenotyping and paternity testing of selected elite f1 hybrids |
| topic | F1 hybrid breeding doubled haploids testing of combining ability paternity determination simple sequence repeats markers |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01111/full |
| work_keys_str_mv | AT katarinarudolfpilih proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids AT markopetkovsek proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids AT jernejjakse proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids AT natasastajner proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids AT janamurovec proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids AT borutbohanec proposalofanewhybridbreedingmethodbasedongenotypinginterpollinationphenotypingandpaternitytestingofselectedelitef1hybrids |