MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from tw...
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MDPI AG
2022-09-01
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| author | Zobia Umair Mi-Ock Baek Jisue Song Seona An Seung Joo Chon Mee-Sup Yoon |
| author_facet | Zobia Umair Mi-Ock Baek Jisue Song Seona An Seung Joo Chon Mee-Sup Yoon |
| author_sort | Zobia Umair |
| collection | DOAJ |
| description | Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary—but not in the uterus—of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI. |
| first_indexed | 2024-03-10T00:26:21Z |
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| id | doaj.art-1dab056878cf4f7b926734fde1640456 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:26:21Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-1dab056878cf4f7b926734fde16404562023-11-23T15:32:22ZengMDPI AGCells2073-44092022-09-011118279710.3390/cells11182797MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian InsufficiencyZobia Umair0Mi-Ock Baek1Jisue Song2Seona An3Seung Joo Chon4Mee-Sup Yoon5Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, KoreaDepartment of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, KoreaGachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, KoreaGachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, KoreaGachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, KoreaDepartment of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, KoreaPremature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary—but not in the uterus—of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI.https://www.mdpi.com/2073-4409/11/18/2797urinary exosomemicro-RNApremature ovarian insufficiencyhsa-miR-4516turner syndrome |
| spellingShingle | Zobia Umair Mi-Ock Baek Jisue Song Seona An Seung Joo Chon Mee-Sup Yoon MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency Cells urinary exosome micro-RNA premature ovarian insufficiency hsa-miR-4516 turner syndrome |
| title | MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency |
| title_full | MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency |
| title_fullStr | MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency |
| title_full_unstemmed | MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency |
| title_short | MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency |
| title_sort | microrna 4516 in urinary exosomes as a biomarker of premature ovarian insufficiency |
| topic | urinary exosome micro-RNA premature ovarian insufficiency hsa-miR-4516 turner syndrome |
| url | https://www.mdpi.com/2073-4409/11/18/2797 |
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