Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism
Scope: The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease. Methods and Results: A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan. Monosaccharide compositi...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/1660-3397/21/1/16 |
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| author | Haitao Xiao Jinxiu Feng Jiao Peng Peigen Wu Yaoyao Chang Xianqian Li Jinhui Wu Haifeng Huang Huan Deng Miao Qiu Yuedong Yang Bin Du |
| author_facet | Haitao Xiao Jinxiu Feng Jiao Peng Peigen Wu Yaoyao Chang Xianqian Li Jinhui Wu Haifeng Huang Huan Deng Miao Qiu Yuedong Yang Bin Du |
| author_sort | Haitao Xiao |
| collection | DOAJ |
| description | Scope: The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease. Methods and Results: A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan. Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→. Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis. The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing. In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice. Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host–microbe tryptophan metabolism of the mice with chronic colitis. Conclusion: Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment. |
| first_indexed | 2024-03-09T11:52:09Z |
| format | Article |
| id | doaj.art-1dabe299d4c74a878ec0eb50da80f9b3 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T11:52:09Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-1dabe299d4c74a878ec0eb50da80f9b32023-11-30T23:13:19ZengMDPI AGMarine Drugs1660-33972022-12-012111610.3390/md21010016Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan MetabolismHaitao Xiao0Jinxiu Feng1Jiao Peng2Peigen Wu3Yaoyao Chang4Xianqian Li5Jinhui Wu6Haifeng Huang7Huan Deng8Miao Qiu9Yuedong Yang10Bin Du11School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaHebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaDepartment of Pharmacy, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaHebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaHebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaHebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaScope: The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease. Methods and Results: A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan. Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→. Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis. The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing. In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice. Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host–microbe tryptophan metabolism of the mice with chronic colitis. Conclusion: Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.https://www.mdpi.com/1660-3397/21/1/16fucoidanultrasonic degradationcolonic inflammationgut microbiotatryptophan metabolism |
| spellingShingle | Haitao Xiao Jinxiu Feng Jiao Peng Peigen Wu Yaoyao Chang Xianqian Li Jinhui Wu Haifeng Huang Huan Deng Miao Qiu Yuedong Yang Bin Du Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism Marine Drugs fucoidan ultrasonic degradation colonic inflammation gut microbiota tryptophan metabolism |
| title | Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism |
| title_full | Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism |
| title_fullStr | Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism |
| title_full_unstemmed | Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism |
| title_short | Fuc-S—A New Ultrasonic Degraded Sulfated α-<span style="font-variant: small-caps">l</span>-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism |
| title_sort | fuc s a new ultrasonic degraded sulfated α span style font variant small caps l span fucooligosaccharide alleviates dss inflicted colitis through reshaping gut microbiota and modulating host microbe tryptophan metabolism |
| topic | fucoidan ultrasonic degradation colonic inflammation gut microbiota tryptophan metabolism |
| url | https://www.mdpi.com/1660-3397/21/1/16 |
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