Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model
Background: Bone marrow stromal cells (BMSCs), as a type of mesenchymal stem cells, and the granulocyte colony-stimulating factor (G-CSF), as a type of growth factor, may recover damaged ovaries. The aim of the present study was to investigate the effects of the coadministration of BMSCs and the G-C...
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Shiraz University of Medical Sciences
2019-03-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/article_44514_15b27269a9907ab1aec9aa645bdb859c.pdf |
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| author | Hamid Reza Sameni Maryam Seiri Manouchehr Safari Mohammad Hasan Tabrizi Amjad Nasrin Khanmohammadi Sam Zarbakhsh |
| author_facet | Hamid Reza Sameni Maryam Seiri Manouchehr Safari Mohammad Hasan Tabrizi Amjad Nasrin Khanmohammadi Sam Zarbakhsh |
| author_sort | Hamid Reza Sameni |
| collection | DOAJ |
| description | Background: Bone marrow stromal cells (BMSCs), as a type of mesenchymal stem cells, and the granulocyte colony-stimulating factor (G-CSF), as a type of growth factor, may recover damaged ovaries. The aim of the present study was to investigate the effects of the coadministration of BMSCs and the G-CSF on damaged ovaries after creating a chemotherapy model with cyclophosphamide (CTX) in rats.Methods: The present study was performed in Semnan, Iran, in the late 2016 and the early 2017. BMSCs were cultured and were confirmed using the CD markers of stromal cells. Forty female Wistar rats were randomly divided into 4 groups. The rats were injected intraperitoneally with CTX for 14 days to induce chemotherapy and ovarian destruction. Then, the BMSCs were injected into bilateral ovaries and the G-CSF was injected intraperitoneally, individually and together. Four weeks later, the number of ovarian follicles using H&E staining, the number of apoptotic granulosa cells using the TUNEL assay, the number of produced oocytes from the ovaries, and the levels of serum E2 and FSH using an ELISA reader were assessed. Statistical analysis was done using one-way ANOVA with SPSS, version 16.0.Results: The results showed that the effects of the coadministration of 2×106 BMSCs and 70 µg/kg of the G-CSF were significantly more favorable than those in the control group (P<0.001), the BMSC group (P=0.016), and the G-CSF group (P<0.001) on the recovery of damaged ovaries. Conclusion: The efficacy of the coadministration of BMSCs and the G-CSF in the recovery of ovaries damaged by chemotherapy was high by comparison with the administration of either of them separately. |
| first_indexed | 2024-12-12T23:40:20Z |
| format | Article |
| id | doaj.art-1dac48b56d7346ebac918317b7024f7e |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-12T23:40:20Z |
| publishDate | 2019-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-1dac48b56d7346ebac918317b7024f7e2022-12-22T00:07:13ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882019-03-0144213514510.30476/ijms.2019.4451444514Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat ModelHamid Reza Sameni0Maryam Seiri1Manouchehr Safari2Mohammad Hasan Tabrizi Amjad3Nasrin Khanmohammadi4Sam Zarbakhsh5Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranNervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranNervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranNervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranNervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranNervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranBackground: Bone marrow stromal cells (BMSCs), as a type of mesenchymal stem cells, and the granulocyte colony-stimulating factor (G-CSF), as a type of growth factor, may recover damaged ovaries. The aim of the present study was to investigate the effects of the coadministration of BMSCs and the G-CSF on damaged ovaries after creating a chemotherapy model with cyclophosphamide (CTX) in rats.Methods: The present study was performed in Semnan, Iran, in the late 2016 and the early 2017. BMSCs were cultured and were confirmed using the CD markers of stromal cells. Forty female Wistar rats were randomly divided into 4 groups. The rats were injected intraperitoneally with CTX for 14 days to induce chemotherapy and ovarian destruction. Then, the BMSCs were injected into bilateral ovaries and the G-CSF was injected intraperitoneally, individually and together. Four weeks later, the number of ovarian follicles using H&E staining, the number of apoptotic granulosa cells using the TUNEL assay, the number of produced oocytes from the ovaries, and the levels of serum E2 and FSH using an ELISA reader were assessed. Statistical analysis was done using one-way ANOVA with SPSS, version 16.0.Results: The results showed that the effects of the coadministration of 2×106 BMSCs and 70 µg/kg of the G-CSF were significantly more favorable than those in the control group (P<0.001), the BMSC group (P=0.016), and the G-CSF group (P<0.001) on the recovery of damaged ovaries. Conclusion: The efficacy of the coadministration of BMSCs and the G-CSF in the recovery of ovaries damaged by chemotherapy was high by comparison with the administration of either of them separately.http://ijms.sums.ac.ir/article_44514_15b27269a9907ab1aec9aa645bdb859c.pdfMesenchymal stromal cellsGranulocyte colony-stimulating factorChemotherapyOvaryRegeneration |
| spellingShingle | Hamid Reza Sameni Maryam Seiri Manouchehr Safari Mohammad Hasan Tabrizi Amjad Nasrin Khanmohammadi Sam Zarbakhsh Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model Iranian Journal of Medical Sciences Mesenchymal stromal cells Granulocyte colony-stimulating factor Chemotherapy Ovary Regeneration |
| title | Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model |
| title_full | Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model |
| title_fullStr | Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model |
| title_full_unstemmed | Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model |
| title_short | Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the Management of Chemotherapy-Induced Ovarian Failure in a Rat Model |
| title_sort | bone marrow stromal cells with the granulocyte colony stimulating factor in the management of chemotherapy induced ovarian failure in a rat model |
| topic | Mesenchymal stromal cells Granulocyte colony-stimulating factor Chemotherapy Ovary Regeneration |
| url | http://ijms.sums.ac.ir/article_44514_15b27269a9907ab1aec9aa645bdb859c.pdf |
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