Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs

Abstract Background Fluralaner (Bravecto™) is a novel systemic insecticide and acaricide that provides long persistent antiparasitic activity following a single administration at the minimum dose of 25 mg/kg body weight. Methods Three negative controlled, randomized studies were conducted in dogs to...

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Main Authors: Janina Taenzler, Christina Wengenmayer, Heike Williams, Josephus Fourie, Eva Zschiesche, Rainer KA Roepke, Anja R Heckeroth
Format: Article
Language:English
Published: BMC 2014-12-01
Series:Parasites & Vectors
Subjects:
Online Access:https://doi.org/10.1186/s13071-014-0567-6
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author Janina Taenzler
Christina Wengenmayer
Heike Williams
Josephus Fourie
Eva Zschiesche
Rainer KA Roepke
Anja R Heckeroth
author_facet Janina Taenzler
Christina Wengenmayer
Heike Williams
Josephus Fourie
Eva Zschiesche
Rainer KA Roepke
Anja R Heckeroth
author_sort Janina Taenzler
collection DOAJ
description Abstract Background Fluralaner (Bravecto™) is a novel systemic insecticide and acaricide that provides long persistent antiparasitic activity following a single administration at the minimum dose of 25 mg/kg body weight. Methods Three negative controlled, randomized studies were conducted in dogs to evaluate the start to kill (1 study) and the speed of flea kill (2 studies) of fluralaner. All dogs were infested prior to treatment with unfed adult C. felis fleas. Dogs in the treated groups were administered once orally with fluralaner at a minimum dose of 25 mg/kg body weight, while dogs in the control groups were not treated. Separate control and treatment groups were paired at each time point of flea assessment. Flea counts were performed by combing dogs at either 0.5, 1, 2, or 4 hours after fluralaner treatment to measure the start to kill. To evaluate the speed of flea kill over 12 weeks, flea counts were performed by combing dogs at either 4, 8, 12, or 24 hours after fluralaner treatment and then at 4, 8, 12, or 24 hours after each flea re-infestations performed at 4, 8, and 12 weeks following treatment. Results In the start to kill study, the fluralaner activity against fleas started already at 1 hour post-treatment (8% numerical efficacy). At 2 and 4 hours post-treatment, the flea reduction was significant with 36.7% and 88% efficacy, respectively. In the speed of kill studies, the efficacy against fleas after fluralaner treatment was 80.5% at 4 hours and remained ≥ 99.4% at 8, 12 and 24 hours. After flea re-infestations in weeks 4, 8 and 12, the efficacy at 4 hours was 96.8, 91.4, and 33.5%, respectively. Efficacy at 8, 12 and 24 hours after flea re-infestations was 98.0-100% for the 12 weeks of the study. Except for 4 hours after the 12-week flea re-infestation, flea reduction was significant for all time points after flea re-infestation. Conclusions Single oral fluralaner administration rapidly eliminates existing flea infestations and provides excellent protection against fleas over 12 weeks following treatment.
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spelling doaj.art-1dadc75030b644dfbc53bc1f93ab63d92023-06-04T11:15:47ZengBMCParasites & Vectors1756-33052014-12-01711410.1186/s13071-014-0567-6Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogsJanina Taenzler0Christina Wengenmayer1Heike Williams2Josephus Fourie3Eva Zschiesche4Rainer KA Roepke5Anja R Heckeroth6MSD Animal Health Innovation GmbHMSD Animal Health Innovation GmbHMSD Animal Health Innovation GmbHClinVet International, Uitsigweg, BainsvleiMSD Animal Health Innovation GmbHMSD Animal Health Innovation GmbHMSD Animal Health Innovation GmbHAbstract Background Fluralaner (Bravecto™) is a novel systemic insecticide and acaricide that provides long persistent antiparasitic activity following a single administration at the minimum dose of 25 mg/kg body weight. Methods Three negative controlled, randomized studies were conducted in dogs to evaluate the start to kill (1 study) and the speed of flea kill (2 studies) of fluralaner. All dogs were infested prior to treatment with unfed adult C. felis fleas. Dogs in the treated groups were administered once orally with fluralaner at a minimum dose of 25 mg/kg body weight, while dogs in the control groups were not treated. Separate control and treatment groups were paired at each time point of flea assessment. Flea counts were performed by combing dogs at either 0.5, 1, 2, or 4 hours after fluralaner treatment to measure the start to kill. To evaluate the speed of flea kill over 12 weeks, flea counts were performed by combing dogs at either 4, 8, 12, or 24 hours after fluralaner treatment and then at 4, 8, 12, or 24 hours after each flea re-infestations performed at 4, 8, and 12 weeks following treatment. Results In the start to kill study, the fluralaner activity against fleas started already at 1 hour post-treatment (8% numerical efficacy). At 2 and 4 hours post-treatment, the flea reduction was significant with 36.7% and 88% efficacy, respectively. In the speed of kill studies, the efficacy against fleas after fluralaner treatment was 80.5% at 4 hours and remained ≥ 99.4% at 8, 12 and 24 hours. After flea re-infestations in weeks 4, 8 and 12, the efficacy at 4 hours was 96.8, 91.4, and 33.5%, respectively. Efficacy at 8, 12 and 24 hours after flea re-infestations was 98.0-100% for the 12 weeks of the study. Except for 4 hours after the 12-week flea re-infestation, flea reduction was significant for all time points after flea re-infestation. Conclusions Single oral fluralaner administration rapidly eliminates existing flea infestations and provides excellent protection against fleas over 12 weeks following treatment.https://doi.org/10.1186/s13071-014-0567-6Bravecto™Chewable tabletsFluralanerOnset of activityStart to killSpeed of kill
spellingShingle Janina Taenzler
Christina Wengenmayer
Heike Williams
Josephus Fourie
Eva Zschiesche
Rainer KA Roepke
Anja R Heckeroth
Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
Parasites & Vectors
Bravecto™
Chewable tablets
Fluralaner
Onset of activity
Start to kill
Speed of kill
title Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
title_full Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
title_fullStr Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
title_full_unstemmed Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
title_short Onset of activity of fluralaner (BRAVECTO™) against Ctenocephalides felis on dogs
title_sort onset of activity of fluralaner bravecto™ against ctenocephalides felis on dogs
topic Bravecto™
Chewable tablets
Fluralaner
Onset of activity
Start to kill
Speed of kill
url https://doi.org/10.1186/s13071-014-0567-6
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