Automated cell segmentation in FIJI® using the DRAQ5 nuclear dye

Abstract Background Image segmentation and quantification are essential steps in quantitative cellular analysis. In this work, we present a fast, customizable, and unsupervised cell segmentation method that is based solely on Fiji (is just ImageJ)®, one of the most commonly used open-source software...

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Bibliographic Details
Main Authors: Mischa Schwendy, Ronald E. Unger, Mischa Bonn, Sapun H. Parekh
Format: Article
Language:English
Published: BMC 2019-01-01
Series:BMC Bioinformatics
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12859-019-2602-2