In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L.
BACKGROUND: The leaf and fruit of Psidium guajava L. are potential for neutraceutical beverage especially for antidiabetic drink. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava. METHODS: Both extracts were investigat...
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2018-08-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/402 |
| _version_ | 1811292887231496192 |
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| author | Adelina Simamora Lusia Paramita Nur Azreen Adit Widodo Santoso Kris Herawan Timotius |
| author_facet | Adelina Simamora Lusia Paramita Nur Azreen Adit Widodo Santoso Kris Herawan Timotius |
| author_sort | Adelina Simamora |
| collection | DOAJ |
| description | BACKGROUND: The leaf and fruit of Psidium guajava L. are potential for neutraceutical beverage especially for antidiabetic drink. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava.
METHODS: Both extracts were investigated for their inhibitory effect on α-glucosidase activity in vitro. Their antioxidant activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferrous ion chelating, reducing power and phosphomolybdate methods.
RESULTS: The IC50 of LE, FEandacarbose as a positive control were 5.67, 428.00 and 823.99 μg/mL, respectively. The enzyme kinetic analysis indicated that LE inhibited α-glucosidase in a competitive inhibition type, similar to that of acarbose. Both extracts showed antioxidant activities, with LE showed stronger activities than FE in all methods. In DPPH method, IC50 of LE and FE were 74.77 and 843.84 μg/mL respectively, compared to 53.24 and 21.36 μg/mL for reference antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (AA), respectively. In ferrous ion chelating activity, the IC50 were 147.07 and 2105.05 μg/mL for LE and FE, whereas ethylenediaminetetraacetic acid (EDTA) as a control sample was 66.50 μg/mL. In reducing power and phosphomolybdate methods, at different concentrations, the activities of LE, FE, and standard compounds showed the following order: AA > BHT > LE > FE.
CONCLUSION: LE from P. guajava exhibited excellent inhibitory activity against α-glucosidase. In addition, LE had better antioxidant acivities than FE. This study can recommend the aqueous extract from P. guajava as a promising candidate for neutraceutical drink for prediabetic and diabetic patients.
KEYWORDS: antioxidant, aqueous extract, α-glucosidase inhibition, guava, Psidium guajava L. |
| first_indexed | 2024-04-13T04:52:13Z |
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| id | doaj.art-1dafb569ba1049e4bba85a3b94b7ce4d |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-04-13T04:52:13Z |
| publishDate | 2018-08-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
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| series | Indonesian Biomedical Journal |
| spelling | doaj.art-1dafb569ba1049e4bba85a3b94b7ce4d2022-12-22T03:01:37ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792018-08-011021566410.18585/inabj.v10i2.402269In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L.Adelina Simamora0Lusia Paramita1Nur Azreen2Adit Widodo Santoso3Kris Herawan Timotius4Department of Biochemistry, Krida Wacana Christian University, Jl. Tanjung Duren Raya No. 4, JakartaDepartment of Biochemistry, Krida Wacana Christian University, Jl. Tanjung Duren Raya No. 4, JakartaDepartment of Biochemistry, Krida Wacana Christian University, Jl. Tanjung Duren Raya No. 4, JakartaDepartment of Herbal Medicine Krida Wacana Christian University, Jl. Tanjung Duren Raya No. 4, JakartaDepartment of Biochemistry, Krida Wacana Christian University, Jl. Tanjung Duren Raya No. 4, JakartaBACKGROUND: The leaf and fruit of Psidium guajava L. are potential for neutraceutical beverage especially for antidiabetic drink. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava. METHODS: Both extracts were investigated for their inhibitory effect on α-glucosidase activity in vitro. Their antioxidant activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferrous ion chelating, reducing power and phosphomolybdate methods. RESULTS: The IC50 of LE, FEandacarbose as a positive control were 5.67, 428.00 and 823.99 μg/mL, respectively. The enzyme kinetic analysis indicated that LE inhibited α-glucosidase in a competitive inhibition type, similar to that of acarbose. Both extracts showed antioxidant activities, with LE showed stronger activities than FE in all methods. In DPPH method, IC50 of LE and FE were 74.77 and 843.84 μg/mL respectively, compared to 53.24 and 21.36 μg/mL for reference antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (AA), respectively. In ferrous ion chelating activity, the IC50 were 147.07 and 2105.05 μg/mL for LE and FE, whereas ethylenediaminetetraacetic acid (EDTA) as a control sample was 66.50 μg/mL. In reducing power and phosphomolybdate methods, at different concentrations, the activities of LE, FE, and standard compounds showed the following order: AA > BHT > LE > FE. CONCLUSION: LE from P. guajava exhibited excellent inhibitory activity against α-glucosidase. In addition, LE had better antioxidant acivities than FE. This study can recommend the aqueous extract from P. guajava as a promising candidate for neutraceutical drink for prediabetic and diabetic patients. KEYWORDS: antioxidant, aqueous extract, α-glucosidase inhibition, guava, Psidium guajava L.https://inabj.org/index.php/ibj/article/view/402 |
| spellingShingle | Adelina Simamora Lusia Paramita Nur Azreen Adit Widodo Santoso Kris Herawan Timotius In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. Indonesian Biomedical Journal |
| title | In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. |
| title_full | In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. |
| title_fullStr | In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. |
| title_full_unstemmed | In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. |
| title_short | In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L. |
| title_sort | in vitro antidiabetic and antioxidant activities of aqueous extract from the leaf and fruit of psidium guajava l |
| url | https://inabj.org/index.php/ibj/article/view/402 |
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