A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria
Bacterial fluorescent labeling is a powerful tool for the diagnosis and treatment of bacterial infections. Here, we present a simple and efficient labeling strategy for Staphylococcus aureus. Intracellular labeling of bacteria was achieved by heat shock using Cyanine 5.5 (Cy5.5) near-infrared-I dyes...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128638/full |
| _version_ | 1811167159335780352 |
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| author | Fei Liu Sijie Chen Yingxin Zou Yong Jiao Ying Tang |
| author_facet | Fei Liu Sijie Chen Yingxin Zou Yong Jiao Ying Tang |
| author_sort | Fei Liu |
| collection | DOAJ |
| description | Bacterial fluorescent labeling is a powerful tool for the diagnosis and treatment of bacterial infections. Here, we present a simple and efficient labeling strategy for Staphylococcus aureus. Intracellular labeling of bacteria was achieved by heat shock using Cyanine 5.5 (Cy5.5) near-infrared-I dyes in S. aureus (Cy5.5@S. aureus). Several key factors, such as Cy5.5 concentration and labeling time, were systematically evaluated. Further, the cytotoxicity of Cy5.5 and the stability of Cy5.5@S. aureus was evaluated by flow cytometry, inverted fluorescence microscopy, and transmission electron microscopy. In addition, Cy5.5@S. aureus were used to explore the phagocytic behavior of RAW264.7 macrophages. These results proved that Cy5.5@S. aureus had a uniform fluorescence intensity and high luminance; additionally, our method had no significant adverse effects on S. aureus compared to unlabeled S. aureus infections. Our method provides researchers with a useful option for analyzing the behavior of S. aureus as an infectious agent. This technique can be broadly applied to study host cell–bacteria interactions at the molecular level, and to in vivo tracing of bacterial infections. |
| first_indexed | 2024-04-10T16:04:28Z |
| format | Article |
| id | doaj.art-1db07db472584628b1c9e049322ba546 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T16:04:28Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1db07db472584628b1c9e049322ba5462023-02-10T06:16:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11286381128638A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteriaFei Liu0Sijie Chen1Yingxin Zou2Yong Jiao3Ying Tang4Naval Medical Center, Naval Medical University, Shanghai, ChinaDepartment of Nursing, The 940th Hospital of Joint Logistic Support Force of People’s Liberation Army (PLA), Lanzhou, ChinaNaval Medical Center, Naval Medical University, Shanghai, ChinaNaval Medical Center, Naval Medical University, Shanghai, ChinaNaval Medical Center, Naval Medical University, Shanghai, ChinaBacterial fluorescent labeling is a powerful tool for the diagnosis and treatment of bacterial infections. Here, we present a simple and efficient labeling strategy for Staphylococcus aureus. Intracellular labeling of bacteria was achieved by heat shock using Cyanine 5.5 (Cy5.5) near-infrared-I dyes in S. aureus (Cy5.5@S. aureus). Several key factors, such as Cy5.5 concentration and labeling time, were systematically evaluated. Further, the cytotoxicity of Cy5.5 and the stability of Cy5.5@S. aureus was evaluated by flow cytometry, inverted fluorescence microscopy, and transmission electron microscopy. In addition, Cy5.5@S. aureus were used to explore the phagocytic behavior of RAW264.7 macrophages. These results proved that Cy5.5@S. aureus had a uniform fluorescence intensity and high luminance; additionally, our method had no significant adverse effects on S. aureus compared to unlabeled S. aureus infections. Our method provides researchers with a useful option for analyzing the behavior of S. aureus as an infectious agent. This technique can be broadly applied to study host cell–bacteria interactions at the molecular level, and to in vivo tracing of bacterial infections.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128638/fullStaphylococcus aureuscyanine 5.5heat shockmacrophagesbacterial labeling |
| spellingShingle | Fei Liu Sijie Chen Yingxin Zou Yong Jiao Ying Tang A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria Frontiers in Microbiology Staphylococcus aureus cyanine 5.5 heat shock macrophages bacterial labeling |
| title | A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria |
| title_full | A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria |
| title_fullStr | A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria |
| title_full_unstemmed | A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria |
| title_short | A simple and efficient fluorescent labeling method in Staphylococcus aureus for real-time tracking of invasive bacteria |
| title_sort | simple and efficient fluorescent labeling method in staphylococcus aureus for real time tracking of invasive bacteria |
| topic | Staphylococcus aureus cyanine 5.5 heat shock macrophages bacterial labeling |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128638/full |
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