Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicke...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2009-01-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013 |
| _version_ | 1818955741823762432 |
|---|---|
| author | Hsin-I Chiang Luc R. Berghman Huaijun Zhou |
| author_facet | Hsin-I Chiang Luc R. Berghman Huaijun Zhou |
| author_sort | Hsin-I Chiang |
| collection | DOAJ |
| description | The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a 36% inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1β was increased at 1 h and 4 h, respectively. These findings suggest a likely inhibitory regulation by NF-kB 1, and could lay the foundation for studying the gene network of the innate immune response of SE infection in chickens. |
| first_indexed | 2024-12-20T10:42:53Z |
| format | Article |
| id | doaj.art-1db2362b2c054578aa35c7a6662654dc |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-20T10:42:53Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-1db2362b2c054578aa35c7a6662654dc2022-12-21T19:43:30ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852009-01-0132350751510.1590/S1415-47572009000300013Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidisHsin-I ChiangLuc R. BerghmanHuaijun ZhouThe NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a 36% inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1β was increased at 1 h and 4 h, respectively. These findings suggest a likely inhibitory regulation by NF-kB 1, and could lay the foundation for studying the gene network of the innate immune response of SE infection in chickens.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013RNA interferenceNF-kBp50macrophageSalmonellachicken |
| spellingShingle | Hsin-I Chiang Luc R. Berghman Huaijun Zhou Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis Genetics and Molecular Biology RNA interference NF-kBp50 macrophage Salmonella chicken |
| title | Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis |
| title_full | Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis |
| title_fullStr | Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis |
| title_full_unstemmed | Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis |
| title_short | Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis |
| title_sort | inhibition of nf kb 1 nf kbp50 by rna interference in chicken macrophage hd11 cell line challenged with salmonella enteritidis |
| topic | RNA interference NF-kBp50 macrophage Salmonella chicken |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013 |
| work_keys_str_mv | AT hsinichiang inhibitionofnfkb1nfkbp50byrnainterferenceinchickenmacrophagehd11celllinechallengedwithsalmonellaenteritidis AT lucrberghman inhibitionofnfkb1nfkbp50byrnainterferenceinchickenmacrophagehd11celllinechallengedwithsalmonellaenteritidis AT huaijunzhou inhibitionofnfkb1nfkbp50byrnainterferenceinchickenmacrophagehd11celllinechallengedwithsalmonellaenteritidis |