PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK
ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas
2015-02-01
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| Series: | Jurnal Riset Kimia |
| Online Access: | http://jrk.fmipa.unand.ac.id/index.php/jrk/article/view/44 |
| _version_ | 1831630854090129408 |
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| author | Anthoni Agustien Jetty Nurhajati Linar Z. Udin Pingkan Aditiawati |
| author_facet | Anthoni Agustien Jetty Nurhajati Linar Z. Udin Pingkan Aditiawati |
| author_sort | Anthoni Agustien |
| collection | DOAJ |
| description | ABSTRAK
Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg
Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03 |
| first_indexed | 2024-12-19T04:50:08Z |
| format | Article |
| id | doaj.art-1db3deca8a3a41f4b86cb8964e141ada |
| institution | Directory Open Access Journal |
| issn | 1978-628X 2476-8960 |
| language | English |
| last_indexed | 2024-12-19T04:50:08Z |
| publishDate | 2015-02-01 |
| publisher | Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas |
| record_format | Article |
| series | Jurnal Riset Kimia |
| spelling | doaj.art-1db3deca8a3a41f4b86cb8964e141ada2022-12-21T20:35:22ZengDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas AndalasJurnal Riset Kimia1978-628X2476-89602015-02-014110.25077/jrk.v4i1.4480PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIKAnthoni AgustienJetty NurhajatiLinar Z. UdinPingkan AditiawatiABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03http://jrk.fmipa.unand.ac.id/index.php/jrk/article/view/44 |
| spellingShingle | Anthoni Agustien Jetty Nurhajati Linar Z. Udin Pingkan Aditiawati PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK Jurnal Riset Kimia |
| title | PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK |
| title_full | PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK |
| title_fullStr | PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK |
| title_full_unstemmed | PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK |
| title_short | PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK |
| title_sort | produksi protease alkali dan keratinase dari brevibacillus agri a 03 termofilik |
| url | http://jrk.fmipa.unand.ac.id/index.php/jrk/article/view/44 |
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