Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique
INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hosp...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2012-04-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200003&lng=en&tlng=en |
| _version_ | 1811243560358379520 |
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| author | Juliana Alves Garcia Sergio Cimerman |
| author_facet | Juliana Alves Garcia Sergio Cimerman |
| author_sort | Juliana Alves Garcia |
| collection | DOAJ |
| description | INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emílio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 ± 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm³ (average: 159 ± 250 cells/mm³). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis. |
| first_indexed | 2024-04-12T14:09:30Z |
| format | Article |
| id | doaj.art-1db3f8371f5e440e897ee0d24ed357d1 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-12T14:09:30Z |
| publishDate | 2012-04-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-1db3f8371f5e440e897ee0d24ed357d12022-12-22T03:29:55ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492012-04-0145215615810.1590/S0037-86822012000200003S0037-86822012000200003Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin techniqueJuliana Alves Garcia0Sergio Cimerman1Instituto de Infectologia Emílio RibasInstituto de Infectologia Emílio RibasINTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emílio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 ± 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm³ (average: 159 ± 250 cells/mm³). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200003&lng=en&tlng=enDientamoeba fragilisDiarréiaAIDSHematoxilina férrica simplificada |
| spellingShingle | Juliana Alves Garcia Sergio Cimerman Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique Revista da Sociedade Brasileira de Medicina Tropical Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada |
| title | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
| title_full | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
| title_fullStr | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
| title_full_unstemmed | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
| title_short | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
| title_sort | detection of dientamoeba fragilis in patients with hiv aids by using a simplified iron hematoxylin technique |
| topic | Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200003&lng=en&tlng=en |
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