| Summary: | Increasing antimicrobial resistance in nosocomial pathogens, such as <i>Acinetobacter baumannii</i>, is becoming a serious threat to public health. It is necessary to detect <i>β</i>-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of <i>β</i>-lactamases in a selected clinical isolate of <i>A. baumannii</i> of ST2<sup>P</sup>/ST195<sup>Ox</sup> and to characterize possible enzymes, as well as its <i>β</i>-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five <i>bla</i> gene alleles, namely, <i>bla</i><sub>ADC-73</sub>, <i>bla</i><sub>TEM-1</sub>, <i>bla</i><sub>OXA-23</sub>, <i>bla</i><sub>OXA-58</sub> and <i>bla</i><sub>OXA-66</sub>, as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian <i>A. baumannii</i> AC30 genome and 76 regions with high homology. This study suggests that resistance to <i>β</i>-lactams in this <i>A. baumannii</i> isolate is mainly due to an overproduction of <i>β</i>-lactamases in combination with other resistance mechanism (efflux pump system).
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