Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer
<p>Abstract</p> <p>Background</p> <p>The secreted protein acidic and rich in cysteine (<it>SPARC</it>) plays a pivotal role in regulating cell-matrix interactions and tumor angiogenesis, proliferation, and migration. Detection of <it>SPARC </it>g...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-03-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/29/1/28 |
| _version_ | 1818641742147092480 |
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| author | Lv Shunli Li Minghui Cao Jia Du Yiqi Li Zhaoshen Huang Haojie Song Jian Gao Jun Lin Han Gong Yanfang |
| author_facet | Lv Shunli Li Minghui Cao Jia Du Yiqi Li Zhaoshen Huang Haojie Song Jian Gao Jun Lin Han Gong Yanfang |
| author_sort | Lv Shunli |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The secreted protein acidic and rich in cysteine (<it>SPARC</it>) plays a pivotal role in regulating cell-matrix interactions and tumor angiogenesis, proliferation, and migration. Detection of <it>SPARC </it>gene methylation may be useful as a tumorigenesis marker for early detection of pancreatic cancer.</p> <p>Methods</p> <p>Methylation of the <it>SPARC </it>gene transcriptional regulation region (TRR) was detected using bisulfite-specific (BSP) PCR-based sequencing analysis in 40 cases of pancreatic cancer and the adjacent normal tissues, 6 chronic pancreatitis tissues, and 6 normal pancreatic tissues. BSP cloning-based sequencing analysis was also performed in selected cases. Clinicopathological data from the cancer patients were collected and analyzed.</p> <p>Results</p> <p>Analysis of <it>SPARC </it>gene TRR methylation showed two hypermethylation wave peak regions: CpG Region 1 (CpG site 1-7) and CpG Region 2 (CpG site 8-12). Pancreatic tissues have shown methylation in both regions with gradual increases from normal, chronic pancreatitis, and adjacent normal tissues to cancerous tissues. However, Methylation of CpG Region 2 was more sensitive than CpG Region 1 in pancreatic tumorigenesis. Furthermore, the methylation level of CpG Region 2 was associated with increased tumor size and exposure to the risk factors (tobacco smoke and alcohol consumption) for developing pancreatic cancer.</p> <p>Conclusion</p> <p>Methylation of the <it>SPARC </it>gene, specifically CpG Region 2, may be an early event during pancreatic tumorigenesis and should be further evaluated as a tumorigenesis marker for early detection of pancreatic cancer.</p> |
| first_indexed | 2024-12-16T23:32:00Z |
| format | Article |
| id | doaj.art-1dba604146944409836e043b32969e56 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-16T23:32:00Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-1dba604146944409836e043b32969e562022-12-21T22:11:51ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662010-03-012912810.1186/1756-9966-29-28Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancerLv ShunliLi MinghuiCao JiaDu YiqiLi ZhaoshenHuang HaojieSong JianGao JunLin HanGong Yanfang<p>Abstract</p> <p>Background</p> <p>The secreted protein acidic and rich in cysteine (<it>SPARC</it>) plays a pivotal role in regulating cell-matrix interactions and tumor angiogenesis, proliferation, and migration. Detection of <it>SPARC </it>gene methylation may be useful as a tumorigenesis marker for early detection of pancreatic cancer.</p> <p>Methods</p> <p>Methylation of the <it>SPARC </it>gene transcriptional regulation region (TRR) was detected using bisulfite-specific (BSP) PCR-based sequencing analysis in 40 cases of pancreatic cancer and the adjacent normal tissues, 6 chronic pancreatitis tissues, and 6 normal pancreatic tissues. BSP cloning-based sequencing analysis was also performed in selected cases. Clinicopathological data from the cancer patients were collected and analyzed.</p> <p>Results</p> <p>Analysis of <it>SPARC </it>gene TRR methylation showed two hypermethylation wave peak regions: CpG Region 1 (CpG site 1-7) and CpG Region 2 (CpG site 8-12). Pancreatic tissues have shown methylation in both regions with gradual increases from normal, chronic pancreatitis, and adjacent normal tissues to cancerous tissues. However, Methylation of CpG Region 2 was more sensitive than CpG Region 1 in pancreatic tumorigenesis. Furthermore, the methylation level of CpG Region 2 was associated with increased tumor size and exposure to the risk factors (tobacco smoke and alcohol consumption) for developing pancreatic cancer.</p> <p>Conclusion</p> <p>Methylation of the <it>SPARC </it>gene, specifically CpG Region 2, may be an early event during pancreatic tumorigenesis and should be further evaluated as a tumorigenesis marker for early detection of pancreatic cancer.</p>http://www.jeccr.com/content/29/1/28 |
| spellingShingle | Lv Shunli Li Minghui Cao Jia Du Yiqi Li Zhaoshen Huang Haojie Song Jian Gao Jun Lin Han Gong Yanfang Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer Journal of Experimental & Clinical Cancer Research |
| title | Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer |
| title_full | Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer |
| title_fullStr | Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer |
| title_full_unstemmed | Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer |
| title_short | Methylation of the <it>SPARC </it>gene promoter and its clinical implication in pancreatic cancer |
| title_sort | methylation of the it sparc it gene promoter and its clinical implication in pancreatic cancer |
| url | http://www.jeccr.com/content/29/1/28 |
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