Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs
The interleukin-4 (IL-4) and interleukin-4 receptor (IL-4R) are cytokines that are involved in the immune and reproductive systems. This study aimed to verify the polymorphisms in the porcine <i>IL-4</i> and <i>IL-4R</i> genes and to assess their effects on litter size traits...
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MDPI AG
2021-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/1154 |
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| author | Worrarak Norseeda Guisheng Liu Tawatchai Teltathum Pantaporn Supakankul Korawan Sringarm Watcharapong Naraballobh Trisadee Khamlor Siriwadee Chomdej Korakot Nganvongpanit Patcharin Krutmuang Supamit Mekchay |
| author_facet | Worrarak Norseeda Guisheng Liu Tawatchai Teltathum Pantaporn Supakankul Korawan Sringarm Watcharapong Naraballobh Trisadee Khamlor Siriwadee Chomdej Korakot Nganvongpanit Patcharin Krutmuang Supamit Mekchay |
| author_sort | Worrarak Norseeda |
| collection | DOAJ |
| description | The interleukin-4 (IL-4) and interleukin-4 receptor (IL-4R) are cytokines that are involved in the immune and reproductive systems. This study aimed to verify the polymorphisms in the porcine <i>IL-4</i> and <i>IL-4R</i> genes and to assess their effects on litter size traits in commercial pigs. Single nucleotide polymorphisms (SNPs) in the porcine <i>IL-4</i> and <i>IL-4R</i> genes were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A non-coding SNP of <i>IL-4</i> g.134993898T > C and a non-synonymous SNP of <i>IL-4R</i> c.1577A > T (amino acid change at position 526, Q526L) were found to be segregating in Landrace sows. The <i>IL-4</i> g.134993898T > C polymorphism was significantly associated with the number of piglets weaned alive (NWA) trait. The <i>IL-4R</i> c.1577A > T polymorphism was significantly associated with the number born alive (NBA) and NWA traits. Moreover, the accumulation of favorable alleles of these two SNP markers revealed significant associations with the NBA, NWA, and mean weight of piglets at weaning (MWW) traits. These findings indicate that the porcine <i>IL-4</i> and <i>IL-4R</i> genes may contribute to the reproductive traits of pigs and could be used as candidate genes to improve litter size traits in the pig breeding industry. |
| first_indexed | 2024-03-10T12:13:10Z |
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| id | doaj.art-1dbeed8d77a346db8ff5d527c36aad55 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:13:10Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1dbeed8d77a346db8ff5d527c36aad552023-11-21T16:00:14ZengMDPI AGAnimals2076-26152021-04-01114115410.3390/ani11041154Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in PigsWorrarak Norseeda0Guisheng Liu1Tawatchai Teltathum2Pantaporn Supakankul3Korawan Sringarm4Watcharapong Naraballobh5Trisadee Khamlor6Siriwadee Chomdej7Korakot Nganvongpanit8Patcharin Krutmuang9Supamit Mekchay10Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandInstitute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaMae Hong Son Livestock Research and Breeding Center, Mae Hong Son 58000, ThailandDivision of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandInnovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandThe interleukin-4 (IL-4) and interleukin-4 receptor (IL-4R) are cytokines that are involved in the immune and reproductive systems. This study aimed to verify the polymorphisms in the porcine <i>IL-4</i> and <i>IL-4R</i> genes and to assess their effects on litter size traits in commercial pigs. Single nucleotide polymorphisms (SNPs) in the porcine <i>IL-4</i> and <i>IL-4R</i> genes were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A non-coding SNP of <i>IL-4</i> g.134993898T > C and a non-synonymous SNP of <i>IL-4R</i> c.1577A > T (amino acid change at position 526, Q526L) were found to be segregating in Landrace sows. The <i>IL-4</i> g.134993898T > C polymorphism was significantly associated with the number of piglets weaned alive (NWA) trait. The <i>IL-4R</i> c.1577A > T polymorphism was significantly associated with the number born alive (NBA) and NWA traits. Moreover, the accumulation of favorable alleles of these two SNP markers revealed significant associations with the NBA, NWA, and mean weight of piglets at weaning (MWW) traits. These findings indicate that the porcine <i>IL-4</i> and <i>IL-4R</i> genes may contribute to the reproductive traits of pigs and could be used as candidate genes to improve litter size traits in the pig breeding industry.https://www.mdpi.com/2076-2615/11/4/1154<i>IL-4</i><i>IL-4R</i>litter sizepigSNPs |
| spellingShingle | Worrarak Norseeda Guisheng Liu Tawatchai Teltathum Pantaporn Supakankul Korawan Sringarm Watcharapong Naraballobh Trisadee Khamlor Siriwadee Chomdej Korakot Nganvongpanit Patcharin Krutmuang Supamit Mekchay Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs Animals <i>IL-4</i> <i>IL-4R</i> litter size pig SNPs |
| title | Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs |
| title_full | Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs |
| title_fullStr | Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs |
| title_full_unstemmed | Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs |
| title_short | Association of <i>IL-4</i> and <i>IL-4R</i> Polymorphisms with Litter Size Traits in Pigs |
| title_sort | association of i il 4 i and i il 4r i polymorphisms with litter size traits in pigs |
| topic | <i>IL-4</i> <i>IL-4R</i> litter size pig SNPs |
| url | https://www.mdpi.com/2076-2615/11/4/1154 |
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