Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
<i>Rickettsia conorii</i> is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of <i>...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/973 |
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| author | Neda Baseri Mostafa Salehi-Vaziri Ehsan Mostafavi Fahimeh Bagheri Amiri Mina Latifian John Stenos Saber Esmaeili |
| author_facet | Neda Baseri Mostafa Salehi-Vaziri Ehsan Mostafavi Fahimeh Bagheri Amiri Mina Latifian John Stenos Saber Esmaeili |
| author_sort | Neda Baseri |
| collection | DOAJ |
| description | <i>Rickettsia conorii</i> is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of <i>R. conorii</i> among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA <i>Rickettsia</i> spp. samples that were classified as <i>R. conorii</i> subsp. <i>conorii</i>, <i>R. conorii</i> subsp. <i>Israelensis,</i> and <i>R. helvetica</i> using the sequencing of <i>gltA</i>, <i>ompA</i>, and <i>17kDa</i> genes. Furthermore, <i>R. conorii</i> IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes. |
| first_indexed | 2024-03-09T22:52:56Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:52:56Z |
| publishDate | 2022-08-01 |
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| series | Pathogens |
| spelling | doaj.art-1dbf1cc6b8fa414cadc26d237e41a5e02023-11-23T18:15:17ZengMDPI AGPathogens2076-08172022-08-0111997310.3390/pathogens11090973Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic FeverNeda Baseri0Mostafa Salehi-Vaziri1Ehsan Mostafavi2Fahimeh Bagheri Amiri3Mina Latifian4John Stenos5Saber Esmaeili6Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Arboviruses & Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, IranAustralian Rickettsial Reference Laboratory, University Hospital Geelong, Geelong, VIC 3220, AustraliaDepartment of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, Iran<i>Rickettsia conorii</i> is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of <i>R. conorii</i> among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA <i>Rickettsia</i> spp. samples that were classified as <i>R. conorii</i> subsp. <i>conorii</i>, <i>R. conorii</i> subsp. <i>Israelensis,</i> and <i>R. helvetica</i> using the sequencing of <i>gltA</i>, <i>ompA</i>, and <i>17kDa</i> genes. Furthermore, <i>R. conorii</i> IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes.https://www.mdpi.com/2076-0817/11/9/973Mediterranean spotted fever<i>Rickettsia conorii</i>Congo-Crimean hemorrhagic fever<i>Rickettsia helvetica</i> |
| spellingShingle | Neda Baseri Mostafa Salehi-Vaziri Ehsan Mostafavi Fahimeh Bagheri Amiri Mina Latifian John Stenos Saber Esmaeili Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever Pathogens Mediterranean spotted fever <i>Rickettsia conorii</i> Congo-Crimean hemorrhagic fever <i>Rickettsia helvetica</i> |
| title | Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
| title_full | Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
| title_fullStr | Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
| title_full_unstemmed | Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
| title_short | Investigation of <i>Rickettsia conorii</i> in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
| title_sort | investigation of i rickettsia conorii i in patients suspected of having crimean congo hemorrhagic fever |
| topic | Mediterranean spotted fever <i>Rickettsia conorii</i> Congo-Crimean hemorrhagic fever <i>Rickettsia helvetica</i> |
| url | https://www.mdpi.com/2076-0817/11/9/973 |
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