Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins
Plasma low density lipoproteins from 20 patients were separated by capillary isotachophoresis (ITP). In each patient the apparent diameter of the predominant LDL peak on whole plasma was also determined by nondenaturing gradient gel electrophoresis. Furthermore the concentration of the more electron...
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| Format: | Article |
| Language: | English |
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Elsevier
1999-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520333538 |
| _version_ | 1818385225893281792 |
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| author | Gabriele Bittolo-Bon Giuseppe Cazzolato |
| author_facet | Gabriele Bittolo-Bon Giuseppe Cazzolato |
| author_sort | Gabriele Bittolo-Bon |
| collection | DOAJ |
| description | Plasma low density lipoproteins from 20 patients were separated by capillary isotachophoresis (ITP). In each patient the apparent diameter of the predominant LDL peak on whole plasma was also determined by nondenaturing gradient gel electrophoresis. Furthermore the concentration of the more electronegatively charged in vivo oxidized LDL− was accomplished using anion exchange high pressure liquid chromatography. By analytical capillary ITP of whole plasma lipoproteins, prestained with a lipophilic dye, LDL were separated into four subfractions. Usually, the predominant subfraction was the slow migrating LDL4, followed by LDL3, and then by the faster LDL2 and LDL1. Slow migrating LDL4 correlated negatively with plasma triglycerides and LDL− and positively with plasma high density lipoprotein (HDL) cholesterol and with the LDL diameter, while the faster LDL1 showed an inverse behavior. The LDL1 + LDL2 to LDL3 + LDL4 ratio showed a strong positive correlation with LDL− concentration (r = 0.87; P < 0.001) and a highly significant inverse correlation with the LDL particle diameter (r = −0.74; P < 0.001). At least three highly atherogenic LDL that could be found in human plasma, namely oxidized, glycated and small-dense, are characterized by a greater electric charge. The LDL profile from capillary ITP and the relative prevalence of faster or slower migrating LDL fractions could indicate the presence of more atherogenic LDL.—Bittolo-Bon, G., and G. Cazzolato. Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins. J. Lipid Res. 1999. 40: 170–177. |
| first_indexed | 2024-12-14T03:34:47Z |
| format | Article |
| id | doaj.art-1dc1756b1b50448793c37eafb28762b0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:34:47Z |
| publishDate | 1999-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-1dc1756b1b50448793c37eafb28762b02022-12-21T23:18:39ZengElsevierJournal of Lipid Research0022-22751999-01-01401170177Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteinsGabriele Bittolo-Bon0Giuseppe Cazzolato1To whom correspondence should be addressed.; Department of Internal Medicine and Metabolic Diseases, Pietro Avogaro Laboratory, Regional Center for Atherosclerosis, Venice General Hospital, C. po SS Giovanni e Paolo. 30122-Venice, ItalyDepartment of Internal Medicine and Metabolic Diseases, Pietro Avogaro Laboratory, Regional Center for Atherosclerosis, Venice General Hospital, C. po SS Giovanni e Paolo. 30122-Venice, ItalyPlasma low density lipoproteins from 20 patients were separated by capillary isotachophoresis (ITP). In each patient the apparent diameter of the predominant LDL peak on whole plasma was also determined by nondenaturing gradient gel electrophoresis. Furthermore the concentration of the more electronegatively charged in vivo oxidized LDL− was accomplished using anion exchange high pressure liquid chromatography. By analytical capillary ITP of whole plasma lipoproteins, prestained with a lipophilic dye, LDL were separated into four subfractions. Usually, the predominant subfraction was the slow migrating LDL4, followed by LDL3, and then by the faster LDL2 and LDL1. Slow migrating LDL4 correlated negatively with plasma triglycerides and LDL− and positively with plasma high density lipoprotein (HDL) cholesterol and with the LDL diameter, while the faster LDL1 showed an inverse behavior. The LDL1 + LDL2 to LDL3 + LDL4 ratio showed a strong positive correlation with LDL− concentration (r = 0.87; P < 0.001) and a highly significant inverse correlation with the LDL particle diameter (r = −0.74; P < 0.001). At least three highly atherogenic LDL that could be found in human plasma, namely oxidized, glycated and small-dense, are characterized by a greater electric charge. The LDL profile from capillary ITP and the relative prevalence of faster or slower migrating LDL fractions could indicate the presence of more atherogenic LDL.—Bittolo-Bon, G., and G. Cazzolato. Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins. J. Lipid Res. 1999. 40: 170–177.http://www.sciencedirect.com/science/article/pii/S0022227520333538oxidized LDLLDL sizelipoprotein subfractionation |
| spellingShingle | Gabriele Bittolo-Bon Giuseppe Cazzolato Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins Journal of Lipid Research oxidized LDL LDL size lipoprotein subfractionation |
| title | Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins |
| title_full | Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins |
| title_fullStr | Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins |
| title_full_unstemmed | Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins |
| title_short | Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins |
| title_sort | analytical capillary isotachophoresis of total plasma lipoproteins a new tool to identify atherogenic low density lipoproteins |
| topic | oxidized LDL LDL size lipoprotein subfractionation |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520333538 |
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