In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles
Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities. Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few reports that have shown the usage of AgNPs for in-vivo antibacteri...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2021-09-01
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| Series: | Nano Biomedicine and Engineering |
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| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v13i3.p229-239 |
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| author | Ibtesam Ghadban Auda Istabreq Mohamed Ali Salman Dalal Abed Al-Sattar Jameelah Ghadban Oduha |
| author_facet | Ibtesam Ghadban Auda Istabreq Mohamed Ali Salman Dalal Abed Al-Sattar Jameelah Ghadban Oduha |
| author_sort | Ibtesam Ghadban Auda |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities. Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy. Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method; the size of Cit-AgNPs was determined by an atomic force microscope (AFM) and was between 15 - 90 nm. Acinetobacter baumannii (A. baumannii) isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs were determined by using A. baumannii. The results showed an additive effect of Cit-AgNPs. Four mice groups were infected with a sub-lethal dose of A. baumannii intraperitoneally, IP. The single daily dose of Cit-AgNPs and imipenem plus Cit-AgNPs combination were administered IP. Imipenem and phosphate buffer saline (PBS) was used as positive control and negative control, respectively. Interestingly, only the PBS-treated group showed growth of A. baumannii in the liver and spleen of sacrificed mice. Histopathologically, Cit-AgNPs showed antibacterial activity and had an additive effect when combined with imipenem in vivo and in vitro. Moreover, the Cit-AgNPs showed dose-dependent activity and the organs differed in the illumination of the toxicity effect of Cit-AgNPs even after high dose administration. In conclusion, Cit-capped AgNPs had antibacterial activity against multiple drug resistant (MDR) A. baumannii but not against K. pneumoniae and E. coli. Cit-capped AgNPs increased the inhibition zone of imipenem in additive effect; the minimum inhibitory concentration and the minimum bactericidal concentration of Cit-capped AgNPs were relatively low. Cit-capped AgNPs eliminated A. baumannii infection in vivo when it was given alone or in combination with imipenem. The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed in the illumination of the inflammatory effect of Cit-AgNPs after high dose administration. It is not recommended to use Cit-capped AgNPs systemically despite their valuable additive antibacterial effect especially with a high dose and the combination with imipenem, Topical administration needs to be evaluated. |
| first_indexed | 2024-03-11T21:43:20Z |
| format | Article |
| id | doaj.art-1dc3bbedd0fe4fcd9eb5afb0315721b4 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:43:20Z |
| publishDate | 2021-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-1dc3bbedd0fe4fcd9eb5afb0315721b42023-09-26T11:04:53ZengTsinghua University PressNano Biomedicine and Engineering2150-55782021-09-0113322923910.5101/nbe.v13i3.p229-239In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver NanoparticlesIbtesam Ghadban Auda0Istabreq Mohamed Ali Salman1Dalal Abed Al-Sattar2Jameelah Ghadban Oduha3Department of Biology, College of Science, Mustansiriyah University, IraqDepartment of Biology, College of Science, Mustansiriyah University, IraqDepartment of Biology, College of Science, Mustansiriyah University, IraqDepartment of Microbiology, Al-Kindy College of Medical - Baghdad University, IraqSilver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities. Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy. Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method; the size of Cit-AgNPs was determined by an atomic force microscope (AFM) and was between 15 - 90 nm. Acinetobacter baumannii (A. baumannii) isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs were determined by using A. baumannii. The results showed an additive effect of Cit-AgNPs. Four mice groups were infected with a sub-lethal dose of A. baumannii intraperitoneally, IP. The single daily dose of Cit-AgNPs and imipenem plus Cit-AgNPs combination were administered IP. Imipenem and phosphate buffer saline (PBS) was used as positive control and negative control, respectively. Interestingly, only the PBS-treated group showed growth of A. baumannii in the liver and spleen of sacrificed mice. Histopathologically, Cit-AgNPs showed antibacterial activity and had an additive effect when combined with imipenem in vivo and in vitro. Moreover, the Cit-AgNPs showed dose-dependent activity and the organs differed in the illumination of the toxicity effect of Cit-AgNPs even after high dose administration. In conclusion, Cit-capped AgNPs had antibacterial activity against multiple drug resistant (MDR) A. baumannii but not against K. pneumoniae and E. coli. Cit-capped AgNPs increased the inhibition zone of imipenem in additive effect; the minimum inhibitory concentration and the minimum bactericidal concentration of Cit-capped AgNPs were relatively low. Cit-capped AgNPs eliminated A. baumannii infection in vivo when it was given alone or in combination with imipenem. The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed in the illumination of the inflammatory effect of Cit-AgNPs after high dose administration. It is not recommended to use Cit-capped AgNPs systemically despite their valuable additive antibacterial effect especially with a high dose and the combination with imipenem, Topical administration needs to be evaluated.https://www.sciopen.com/article/10.5101/nbe.v13i3.p229-239citrate capped silver nanoparticlesacinetobacter baumanniireca genein vivoin vitro |
| spellingShingle | Ibtesam Ghadban Auda Istabreq Mohamed Ali Salman Dalal Abed Al-Sattar Jameelah Ghadban Oduha In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles Nano Biomedicine and Engineering citrate capped silver nanoparticles acinetobacter baumannii reca gene in vivo in vitro |
| title | In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles |
| title_full | In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles |
| title_fullStr | In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles |
| title_full_unstemmed | In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles |
| title_short | In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles |
| title_sort | in vivo and in vitro anti acinetobacter baumannii activity of citrate capped silver nanoparticles |
| topic | citrate capped silver nanoparticles acinetobacter baumannii reca gene in vivo in vitro |
| url | https://www.sciopen.com/article/10.5101/nbe.v13i3.p229-239 |
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