Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats
Introduction: Betaine has been demonstrated to have methyl donor and antioxidant properties in our previous reports. Thus, the aim of the present study was to determine plasma homocysteine concentration and evaluate antioxidant activity of betaine following levodopa and benserazide administration, w...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2015-02-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1819-1&slc_lang=en&sid=1 |
| _version_ | 1831539387495612416 |
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| author | M Alirezaei K Chehari |
| author_facet | M Alirezaei K Chehari |
| author_sort | M Alirezaei |
| collection | DOAJ |
| description | Introduction: Betaine has been demonstrated to have methyl donor and antioxidant properties in our previous reports. Thus, the aim of the present study was to determine plasma homocysteine concentration and evaluate antioxidant activity of betaine following levodopa and benserazide administration, which routinely are used in treatment of Parkinson’s disease in liver of rats.
Methods: Sprague–Dawley male rats were treated by levodopa (LD), Betaine (Bet.), levodopa plus betaine (LD/Bet.), levodopa plus benserazide (LD/Ben.), levodopa plus betaine-benserazide (LD/Bet.-Ben.) and distilled water to controls for 10 consecutive days, orally. Plasma homocysteine concentration was measured by ELISA method. Moreover, antioxidant enzyme activities and lipid peroxidation amount were measured via chemical methods. Serumic dopamine concentration was also determined by HPLC method and data were analyzed by One-way ANOVA test.
Results: The study results indicated that the treatment of rats with levodopa and benserazide significantly increased total homocysteine (tHcy) in plasma of the LD/Ben. group in comparison with the other groups (p <0.05). tHcy concentration was also significantly higher in LD group in comparison with control, betaine and LD/Bet. groups. Lipid peroxidation (TBARS) amount of liver increased significantly in LD/Ben. group when compared to the control group which this index decreased by betaine treatment. In contrast, glutathione peroxidase and superoxide dismutase activities in liver were significantly higher in the LD-treated rats as compared to the LD/Ben. group. Serumic dopamine concentration decreased significantly in LD/Ben.-treated rats in comparison with LD and LD/Bet. groups.
Conclusion: Taken together, it seems that betaine acts as an antioxidant agent regarding decrease of LD/Ben.-induced oxidative stress and is able to decrease their oxidative effects in liver of rats. |
| first_indexed | 2024-12-16T23:40:19Z |
| format | Article |
| id | doaj.art-1dc4ed91cfd64f959f7eab8f386371ee |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-16T23:40:19Z |
| publishDate | 2015-02-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-1dc4ed91cfd64f959f7eab8f386371ee2022-12-21T22:11:38ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332015-02-0122617121724Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in RatsM Alirezaei0K Chehari1 Lorestan University lorestan University Introduction: Betaine has been demonstrated to have methyl donor and antioxidant properties in our previous reports. Thus, the aim of the present study was to determine plasma homocysteine concentration and evaluate antioxidant activity of betaine following levodopa and benserazide administration, which routinely are used in treatment of Parkinson’s disease in liver of rats. Methods: Sprague–Dawley male rats were treated by levodopa (LD), Betaine (Bet.), levodopa plus betaine (LD/Bet.), levodopa plus benserazide (LD/Ben.), levodopa plus betaine-benserazide (LD/Bet.-Ben.) and distilled water to controls for 10 consecutive days, orally. Plasma homocysteine concentration was measured by ELISA method. Moreover, antioxidant enzyme activities and lipid peroxidation amount were measured via chemical methods. Serumic dopamine concentration was also determined by HPLC method and data were analyzed by One-way ANOVA test. Results: The study results indicated that the treatment of rats with levodopa and benserazide significantly increased total homocysteine (tHcy) in plasma of the LD/Ben. group in comparison with the other groups (p <0.05). tHcy concentration was also significantly higher in LD group in comparison with control, betaine and LD/Bet. groups. Lipid peroxidation (TBARS) amount of liver increased significantly in LD/Ben. group when compared to the control group which this index decreased by betaine treatment. In contrast, glutathione peroxidase and superoxide dismutase activities in liver were significantly higher in the LD-treated rats as compared to the LD/Ben. group. Serumic dopamine concentration decreased significantly in LD/Ben.-treated rats in comparison with LD and LD/Bet. groups. Conclusion: Taken together, it seems that betaine acts as an antioxidant agent regarding decrease of LD/Ben.-induced oxidative stress and is able to decrease their oxidative effects in liver of rats.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1819-1&slc_lang=en&sid=1Benserazide Betaine Dopamine Homocysteine Levodopa Liver |
| spellingShingle | M Alirezaei K Chehari Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Benserazide Betaine Dopamine Homocysteine Levodopa Liver |
| title | Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats |
| title_full | Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats |
| title_fullStr | Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats |
| title_full_unstemmed | Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats |
| title_short | Hepatoprotective Effects of Betaine Against Oxidative Stress Induced by Levodopa and Benserazide in Rats |
| title_sort | hepatoprotective effects of betaine against oxidative stress induced by levodopa and benserazide in rats |
| topic | Benserazide Betaine Dopamine Homocysteine Levodopa Liver |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1819-1&slc_lang=en&sid=1 |
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