Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene
Epilepsy of infancy with migrating focal seizures (EIMFS) is a kind of epileptic encephalopathy with high genetic heterogeneity. The most common pathogenic gene for EIMFS is potassium sodium-activated channel subfamily T member 1 (KCNT1). Using Sendai virus-mediated reprogramming, we established an...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122001477 |
| _version_ | 1811242345719398400 |
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| author | Xiaona Luo Yilin Wang Fang Yuan Longlong Lin Anqi Wang Chao Wang Miao Guo Simei Wang Chunmei Wang Quanmei Xu Rongrong Yin Hongyi Cheng Yuanfeng Zhang Zhiping Liu Wuhen Xu Jingbin Yan Fanyi Zeng Yucai Chen |
| author_facet | Xiaona Luo Yilin Wang Fang Yuan Longlong Lin Anqi Wang Chao Wang Miao Guo Simei Wang Chunmei Wang Quanmei Xu Rongrong Yin Hongyi Cheng Yuanfeng Zhang Zhiping Liu Wuhen Xu Jingbin Yan Fanyi Zeng Yucai Chen |
| author_sort | Xiaona Luo |
| collection | DOAJ |
| description | Epilepsy of infancy with migrating focal seizures (EIMFS) is a kind of epileptic encephalopathy with high genetic heterogeneity. The most common pathogenic gene for EIMFS is potassium sodium-activated channel subfamily T member 1 (KCNT1). Using Sendai virus-mediated reprogramming, we established an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a five-month-old Chinese girl with heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene. The iPSCs were stable during amplification, expressed pluripotent genes, maintained a normal karyotype, and showed characteristics of the three germs layers in an in vitro differentiation assay. |
| first_indexed | 2024-04-12T13:50:06Z |
| format | Article |
| id | doaj.art-1dc604a960284801bdf68e662f6e9500 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-12T13:50:06Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-1dc604a960284801bdf68e662f6e95002022-12-22T03:30:34ZengElsevierStem Cell Research1873-50612022-07-0162102798Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 geneXiaona Luo0Yilin Wang1Fang Yuan2Longlong Lin3Anqi Wang4Chao Wang5Miao Guo6Simei Wang7Chunmei Wang8Quanmei Xu9Rongrong Yin10Hongyi Cheng11Yuanfeng Zhang12Zhiping Liu13Wuhen Xu14Jingbin Yan15Fanyi Zeng16Yucai Chen17Department of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, ChinaShanghai Key Laboratory of Embryo and Reproduction Engineering, Key Laboratory of Embryo Molecular Biology of National Health Commission, Shanghai Institute of Medical Genetics, Shanghai Chlidren’s Hospital, Shanghai JiaoTong University, Shanghai 200040, ChinaShanghai Key Laboratory of Embryo and Reproduction Engineering, Key Laboratory of Embryo Molecular Biology of National Health Commission, Shanghai Institute of Medical Genetics, Shanghai Chlidren’s Hospital, Shanghai JiaoTong University, Shanghai 200040, ChinaDepartment of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, China; Shanghai Key Laboratory of Embryo and Reproduction Engineering, Key Laboratory of Embryo Molecular Biology of National Health Commission, Shanghai Institute of Medical Genetics, Shanghai Chlidren’s Hospital, Shanghai JiaoTong University, Shanghai 200040, China; Corresponding author at: Department of Neurology, Shanghai Children’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200062, China.Epilepsy of infancy with migrating focal seizures (EIMFS) is a kind of epileptic encephalopathy with high genetic heterogeneity. The most common pathogenic gene for EIMFS is potassium sodium-activated channel subfamily T member 1 (KCNT1). Using Sendai virus-mediated reprogramming, we established an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a five-month-old Chinese girl with heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene. The iPSCs were stable during amplification, expressed pluripotent genes, maintained a normal karyotype, and showed characteristics of the three germs layers in an in vitro differentiation assay.http://www.sciencedirect.com/science/article/pii/S1873506122001477 |
| spellingShingle | Xiaona Luo Yilin Wang Fang Yuan Longlong Lin Anqi Wang Chao Wang Miao Guo Simei Wang Chunmei Wang Quanmei Xu Rongrong Yin Hongyi Cheng Yuanfeng Zhang Zhiping Liu Wuhen Xu Jingbin Yan Fanyi Zeng Yucai Chen Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene Stem Cell Research |
| title | Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene |
| title_full | Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene |
| title_fullStr | Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene |
| title_full_unstemmed | Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene |
| title_short | Induced pluripotent stem cells (SHCDNi006-A cells) isolated from the peripheral blood mononuclear cells of a five-month-old Chinese girl with the heterozygous missense mutation (c.2800 G>A) in the KCNT1 gene |
| title_sort | induced pluripotent stem cells shcdni006 a cells isolated from the peripheral blood mononuclear cells of a five month old chinese girl with the heterozygous missense mutation c 2800 g a in the kcnt1 gene |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122001477 |
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