False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
Abstract The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA1c) measurements. There have been a few reports of compound Hb variants affecting HbA1c testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-03-01
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| Series: | Journal of Diabetes Investigation |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jdi.13971 |
| _version_ | 1797895059254804480 |
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| author | Youqiong Li Jianling Tang Lihong Zheng Kaijian Tang Lingli Li |
| author_facet | Youqiong Li Jianling Tang Lihong Zheng Kaijian Tang Lingli Li |
| author_sort | Youqiong Li |
| collection | DOAJ |
| description | Abstract The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA1c) measurements. There have been a few reports of compound Hb variants affecting HbA1c testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance liquid chromatography with the Hb program showed a high HbA2 level. Similarly, an E‐window peak was separated on the high‐performance liquid chromatography with a glycated Hb program. However, capillary electrophoresis showed two abnormal peaks and no HbA peak. Sanger sequencing confirmed the presence of Hb New York and HbE. This is the first report of a compound heterozygote for HbE and Hb New York. The double heterozygote caused erroneous results for HbA1c on high‐performance liquid chromatography and enzyme assay. |
| first_indexed | 2024-04-10T07:20:23Z |
| format | Article |
| id | doaj.art-1dc66c7bd1814f7d84b7c0f96d976812 |
| institution | Directory Open Access Journal |
| issn | 2040-1116 2040-1124 |
| language | English |
| last_indexed | 2024-04-10T07:20:23Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Investigation |
| spelling | doaj.art-1dc66c7bd1814f7d84b7c0f96d9768122023-02-24T12:37:44ZengWileyJournal of Diabetes Investigation2040-11162040-11242023-03-0114349449710.1111/jdi.13971False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New YorkYouqiong Li0Jianling Tang1Lihong Zheng2Kaijian Tang3Lingli Li4Center for Medical Genetics and Prenatal Diagnosis People's Hospital of Guangxi Zhuang Autonomous Region Nanning Guangxi ChinaDepartment of Clinical Laboratory Women and Children Care Hospital of Xing'an County Guilin Guangxi ChinaCenter for Medical Genetics and Prenatal Diagnosis People's Hospital of Guangxi Zhuang Autonomous Region Nanning Guangxi ChinaDepartment of Clinical Laboratory Women and Children Care Hospital of Xing'an County Guilin Guangxi ChinaDepartment of Clinical Laboratory Women and Children Care Hospital of Xing'an County Guilin Guangxi ChinaAbstract The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA1c) measurements. There have been a few reports of compound Hb variants affecting HbA1c testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance liquid chromatography with the Hb program showed a high HbA2 level. Similarly, an E‐window peak was separated on the high‐performance liquid chromatography with a glycated Hb program. However, capillary electrophoresis showed two abnormal peaks and no HbA peak. Sanger sequencing confirmed the presence of Hb New York and HbE. This is the first report of a compound heterozygote for HbE and Hb New York. The double heterozygote caused erroneous results for HbA1c on high‐performance liquid chromatography and enzyme assay.https://doi.org/10.1111/jdi.13971HbA1cHbEHb New York |
| spellingShingle | Youqiong Li Jianling Tang Lihong Zheng Kaijian Tang Lingli Li False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York Journal of Diabetes Investigation HbA1c HbE Hb New York |
| title | False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York |
| title_full | False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York |
| title_fullStr | False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York |
| title_full_unstemmed | False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York |
| title_short | False hemoglobin A1c value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York |
| title_sort | false hemoglobin a1c value as a result of compound heterozygotes of hemoglobin e and hemoglobin new york |
| topic | HbA1c HbE Hb New York |
| url | https://doi.org/10.1111/jdi.13971 |
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