Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy
Abstract Cancer therapeutic vaccine can induce antigen-specific immune response, which has shown great potential in cancer immunotherapy. As the key factor of vaccine, antigen plays a central role in eliciting antitumor immunity. However, the insufficient antigen delivery and low efficiency of antig...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02106-8 |
| _version_ | 1797451589225545728 |
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| author | Chen Shi Chen Jian Lulu Wang Chen Gao Ting Yang Zhiwen Fu Tingting Wu |
| author_facet | Chen Shi Chen Jian Lulu Wang Chen Gao Ting Yang Zhiwen Fu Tingting Wu |
| author_sort | Chen Shi |
| collection | DOAJ |
| description | Abstract Cancer therapeutic vaccine can induce antigen-specific immune response, which has shown great potential in cancer immunotherapy. As the key factor of vaccine, antigen plays a central role in eliciting antitumor immunity. However, the insufficient antigen delivery and low efficiency of antigen presentation by dendritic cells (DCs) have greatly restricted the therapeutic efficiency of vaccine. Here we developed a kind of DC hybrid zinc phosphate nanoparticles to co-deliver antigenic peptide and photosensitive melanin. Owing to the chelating ability of Zn2+, the nanoparticles can co-encapsulate antigenic peptide and melanin with high efficiency. The nanovaccine showed good physiological stability with the hydration particle size was approximately 30 nm, and zeta potential was around − 10 mV. The nanovaccine showed homologous targeting effect to DCs in vivo and in vitro, efficiently delivering antigen to DCs. Meanwhile, the nanovaccine could effectively reflux to the tumor-draining lymph nodes. When combined with near-infrared irradiation, the nanovaccine induced effective mild heat in vitro and in vivo to promote antigen presentation. After administrating to MC38 tumor-bearing mice, the hybrid nanovaccine effectively promoted the maturation of DCs, the expansion of cytotoxic T lymphocytes and helper T cells, and the secretion of immunostimulatory cytokines, thereby significantly inhibiting tumor growth. Graphical Abstract |
| first_indexed | 2024-03-09T14:56:51Z |
| format | Article |
| id | doaj.art-1dc7b7f10ed248a2b4ba774ef0fb2c84 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-09T14:56:51Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-1dc7b7f10ed248a2b4ba774ef0fb2c842023-11-26T14:08:26ZengBMCJournal of Nanobiotechnology1477-31552023-09-0121112010.1186/s12951-023-02106-8Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapyChen Shi0Chen Jian1Lulu Wang2Chen Gao3Ting Yang4Zhiwen Fu5Tingting Wu6Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAffiliated Hospital of Yunnan UniversityDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Cancer therapeutic vaccine can induce antigen-specific immune response, which has shown great potential in cancer immunotherapy. As the key factor of vaccine, antigen plays a central role in eliciting antitumor immunity. However, the insufficient antigen delivery and low efficiency of antigen presentation by dendritic cells (DCs) have greatly restricted the therapeutic efficiency of vaccine. Here we developed a kind of DC hybrid zinc phosphate nanoparticles to co-deliver antigenic peptide and photosensitive melanin. Owing to the chelating ability of Zn2+, the nanoparticles can co-encapsulate antigenic peptide and melanin with high efficiency. The nanovaccine showed good physiological stability with the hydration particle size was approximately 30 nm, and zeta potential was around − 10 mV. The nanovaccine showed homologous targeting effect to DCs in vivo and in vitro, efficiently delivering antigen to DCs. Meanwhile, the nanovaccine could effectively reflux to the tumor-draining lymph nodes. When combined with near-infrared irradiation, the nanovaccine induced effective mild heat in vitro and in vivo to promote antigen presentation. After administrating to MC38 tumor-bearing mice, the hybrid nanovaccine effectively promoted the maturation of DCs, the expansion of cytotoxic T lymphocytes and helper T cells, and the secretion of immunostimulatory cytokines, thereby significantly inhibiting tumor growth. Graphical Abstracthttps://doi.org/10.1186/s12951-023-02106-8Dendritic cellMild heatCell membraneImmunotherapyNanovaccine |
| spellingShingle | Chen Shi Chen Jian Lulu Wang Chen Gao Ting Yang Zhiwen Fu Tingting Wu Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy Journal of Nanobiotechnology Dendritic cell Mild heat Cell membrane Immunotherapy Nanovaccine |
| title | Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| title_full | Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| title_fullStr | Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| title_full_unstemmed | Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| title_short | Dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| title_sort | dendritic cell hybrid nanovaccine for mild heat inspired cancer immunotherapy |
| topic | Dendritic cell Mild heat Cell membrane Immunotherapy Nanovaccine |
| url | https://doi.org/10.1186/s12951-023-02106-8 |
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