Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
CTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP an...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.789189/full |
| _version_ | 1818693059763765248 |
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| author | Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann |
| author_facet | Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann |
| author_sort | Leo Bellin |
| collection | DOAJ |
| description | CTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP and its deoxy form dCTP are present at low levels in developing seedlings. Thus, under conditions of fast proliferation, the synthesis of CTP (dCTP) can become a limiting factor for RNA and DNA synthesis. The higher sensitivity of ami-CTPS2 lines toward the DNA-Gyrase inhibitor ciprofloxacin, together with reduced plastid DNA copy number and 16S and 23S chloroplast ribosomal RNA support this view. High expression and proposed beneficial biochemical features render CTPS2 the most important isoform for early seedling development. In addition, CTPS2 was identified as an essential enzyme in embryo development before, as knock-out mutants were embryo lethal. In line with this, ami-CTPS2 lines also exhibited reduced seed numbers per plant. |
| first_indexed | 2024-12-17T13:07:40Z |
| format | Article |
| id | doaj.art-1dc81fe146a74a82a3c69984215f8f29 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T13:07:40Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1dc81fe146a74a82a3c69984215f8f292022-12-21T21:47:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-11-011210.3389/fpls.2021.789189789189Cytosolic CTP Production Limits the Establishment of Photosynthesis in ArabidopsisLeo Bellin0Vanessa Scherer1Eva Dörfer2Anne Lau3Alexandre Magno Vicente4Jörg Meurer5Daniel Hickl6Torsten Möhlmann7Plant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyPlant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyPlant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyPlant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyPlant Molecular Biology, Faculty of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, GermanyPlant Molecular Biology, Faculty of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, GermanyPlant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyPlant Physiology, Faculty of Biology, University of Kaiserslautern, Kaiserslautern, GermanyCTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP and its deoxy form dCTP are present at low levels in developing seedlings. Thus, under conditions of fast proliferation, the synthesis of CTP (dCTP) can become a limiting factor for RNA and DNA synthesis. The higher sensitivity of ami-CTPS2 lines toward the DNA-Gyrase inhibitor ciprofloxacin, together with reduced plastid DNA copy number and 16S and 23S chloroplast ribosomal RNA support this view. High expression and proposed beneficial biochemical features render CTPS2 the most important isoform for early seedling development. In addition, CTPS2 was identified as an essential enzyme in embryo development before, as knock-out mutants were embryo lethal. In line with this, ami-CTPS2 lines also exhibited reduced seed numbers per plant.https://www.frontiersin.org/articles/10.3389/fpls.2021.789189/fullpyrimidine de novo synthesisCTPphotosynthesischloroplastgenomedeoxycytidine |
| spellingShingle | Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis Frontiers in Plant Science pyrimidine de novo synthesis CTP photosynthesis chloroplast genome deoxycytidine |
| title | Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis |
| title_full | Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis |
| title_fullStr | Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis |
| title_full_unstemmed | Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis |
| title_short | Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis |
| title_sort | cytosolic ctp production limits the establishment of photosynthesis in arabidopsis |
| topic | pyrimidine de novo synthesis CTP photosynthesis chloroplast genome deoxycytidine |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.789189/full |
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