Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Embrapa Informação Tecnológica
2000-01-01
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| Series: | Pesquisa Agropecuária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015 |
| _version_ | 1817969905458216960 |
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| author | NEPOMUCENO ALEXANDRE LIMA STEWART JAMES MCD OOSTERHUIS DERRICK TURLEY RICKIE NEUMAIER MORMAN FARIAS JOSÉ RENATO BOUÇAS |
| author_facet | NEPOMUCENO ALEXANDRE LIMA STEWART JAMES MCD OOSTERHUIS DERRICK TURLEY RICKIE NEUMAIER MORMAN FARIAS JOSÉ RENATO BOUÇAS |
| author_sort | NEPOMUCENO ALEXANDRE LIMA |
| collection | DOAJ |
| description | The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription. |
| first_indexed | 2024-04-13T20:27:24Z |
| format | Article |
| id | doaj.art-1dc930ce02ad48bc87ecd1837f7104ab |
| institution | Directory Open Access Journal |
| issn | 0100-204X 1678-3921 |
| language | English |
| last_indexed | 2024-04-13T20:27:24Z |
| publishDate | 2000-01-01 |
| publisher | Embrapa Informação Tecnológica |
| record_format | Article |
| series | Pesquisa Agropecuária Brasileira |
| spelling | doaj.art-1dc930ce02ad48bc87ecd1837f7104ab2022-12-22T02:31:17ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira0100-204X1678-39212000-01-0135714071416Isolation of a cotton NADP(H) oxidase homologue induced by drought stressNEPOMUCENO ALEXANDRE LIMASTEWART JAMES MCDOOSTERHUIS DERRICKTURLEY RICKIENEUMAIER MORMANFARIAS JOSÉ RENATO BOUÇASThe aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015messenger RNAgene expressiongenetic transformationwater deficit |
| spellingShingle | NEPOMUCENO ALEXANDRE LIMA STEWART JAMES MCD OOSTERHUIS DERRICK TURLEY RICKIE NEUMAIER MORMAN FARIAS JOSÉ RENATO BOUÇAS Isolation of a cotton NADP(H) oxidase homologue induced by drought stress Pesquisa Agropecuária Brasileira messenger RNA gene expression genetic transformation water deficit |
| title | Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
| title_full | Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
| title_fullStr | Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
| title_full_unstemmed | Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
| title_short | Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
| title_sort | isolation of a cotton nadp h oxidase homologue induced by drought stress |
| topic | messenger RNA gene expression genetic transformation water deficit |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015 |
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