Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella
Abstract Background Eimeria tenella is an obligate intracellular apicomplexan protozoan parasite that has a complex life-cycle. Calcium ions, through various calcium-dependent protein kinases (CDPKs), regulate key events in parasite growth and development, including protein secretion, movement, diff...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-04-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-018-2848-y |
| _version_ | 1811300604271656960 |
|---|---|
| author | Ling Lv Bing Huang Qiping Zhao Zongping Zhao Hui Dong Shunhai Zhu Ting Chen Ming Yan Hongyu Han |
| author_facet | Ling Lv Bing Huang Qiping Zhao Zongping Zhao Hui Dong Shunhai Zhu Ting Chen Ming Yan Hongyu Han |
| author_sort | Ling Lv |
| collection | DOAJ |
| description | Abstract Background Eimeria tenella is an obligate intracellular apicomplexan protozoan parasite that has a complex life-cycle. Calcium ions, through various calcium-dependent protein kinases (CDPKs), regulate key events in parasite growth and development, including protein secretion, movement, differentiation, and invasion of and escape from host cells. In this study, we identified proteins that interact with EtCDPK4 to lay a foundation for clarifying the role of CDPKs in calcium channels. Methods Eimeria tenella merozoites were collected to construct a yeast two-hybrid (Y2H) cDNA library. The Y2H system was used to identify proteins that interact with EtCDPK4. One of interacting proteins was confirmed using bimolecular fluorescence complementation and co-immunoprecipitation in vivo. Co-localization of proteins was performed using immunofluorescence assays. Results Eight proteins that interact with EtCDPK4 were identified using the Y2H system. One of the proteins, E. tenella serine protease inhibitor 1 (EtSerpin), was further confirmed. Conclusion In this study, we screened for proteins that interact with EtCDPK4. An interaction between EtSerpin and EtCDPK4 was identified that may contribute to the invasion and development of E. tenella in host cells. |
| first_indexed | 2024-04-13T06:54:47Z |
| format | Article |
| id | doaj.art-1dca9f830c7343939de5a02143229f5e |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T06:54:47Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-1dca9f830c7343939de5a02143229f5e2022-12-22T02:57:18ZengBMCParasites & Vectors1756-33052018-04-0111111010.1186/s13071-018-2848-yIdentification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenellaLing Lv0Bing Huang1Qiping Zhao2Zongping Zhao3Hui Dong4Shunhai Zhu5Ting Chen6Ming Yan7Hongyu Han8College of Life and Environment Sciences, Shanghai Normal UniversityCollege of Life and Environment Sciences, Shanghai Normal UniversityShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureCollege of Life and Environment Sciences, Shanghai Normal UniversityShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of AgricultureAbstract Background Eimeria tenella is an obligate intracellular apicomplexan protozoan parasite that has a complex life-cycle. Calcium ions, through various calcium-dependent protein kinases (CDPKs), regulate key events in parasite growth and development, including protein secretion, movement, differentiation, and invasion of and escape from host cells. In this study, we identified proteins that interact with EtCDPK4 to lay a foundation for clarifying the role of CDPKs in calcium channels. Methods Eimeria tenella merozoites were collected to construct a yeast two-hybrid (Y2H) cDNA library. The Y2H system was used to identify proteins that interact with EtCDPK4. One of interacting proteins was confirmed using bimolecular fluorescence complementation and co-immunoprecipitation in vivo. Co-localization of proteins was performed using immunofluorescence assays. Results Eight proteins that interact with EtCDPK4 were identified using the Y2H system. One of the proteins, E. tenella serine protease inhibitor 1 (EtSerpin), was further confirmed. Conclusion In this study, we screened for proteins that interact with EtCDPK4. An interaction between EtSerpin and EtCDPK4 was identified that may contribute to the invasion and development of E. tenella in host cells.http://link.springer.com/article/10.1186/s13071-018-2848-yEimeria tenellaCalcium-dependent protein kinasesSerpinYeast two-hybridBimolecular fluorescence complementationCo-immunoprecipitation |
| spellingShingle | Ling Lv Bing Huang Qiping Zhao Zongping Zhao Hui Dong Shunhai Zhu Ting Chen Ming Yan Hongyu Han Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella Parasites & Vectors Eimeria tenella Calcium-dependent protein kinases Serpin Yeast two-hybrid Bimolecular fluorescence complementation Co-immunoprecipitation |
| title | Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella |
| title_full | Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella |
| title_fullStr | Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella |
| title_full_unstemmed | Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella |
| title_short | Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella |
| title_sort | identification of an interaction between calcium dependent protein kinase 4 etcdpk4 and serine protease inhibitor etserpin in eimeria tenella |
| topic | Eimeria tenella Calcium-dependent protein kinases Serpin Yeast two-hybrid Bimolecular fluorescence complementation Co-immunoprecipitation |
| url | http://link.springer.com/article/10.1186/s13071-018-2848-y |
| work_keys_str_mv | AT linglv identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT binghuang identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT qipingzhao identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT zongpingzhao identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT huidong identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT shunhaizhu identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT tingchen identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT mingyan identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella AT hongyuhan identificationofaninteractionbetweencalciumdependentproteinkinase4etcdpk4andserineproteaseinhibitoretserpinineimeriatenella |