The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study
Obesity is a multifactorial disease that can lead to other health issues. Glucagon-like peptide-1(GLP-1), as one of the satiety signal, has been linked with appetite suppression and weight loss. Due to the limitations of GLP-1 and its analogues, alternative treatments such as herbal therapies have b...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2854 |
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| author | Shiqi Luo Harsharn Gill Bryce Feltis Andrew Hung Linh Toan Nguyen George Binh Lenon |
| author_facet | Shiqi Luo Harsharn Gill Bryce Feltis Andrew Hung Linh Toan Nguyen George Binh Lenon |
| author_sort | Shiqi Luo |
| collection | DOAJ |
| description | Obesity is a multifactorial disease that can lead to other health issues. Glucagon-like peptide-1(GLP-1), as one of the satiety signal, has been linked with appetite suppression and weight loss. Due to the limitations of GLP-1 and its analogues, alternative treatments such as herbal therapies have become popular. The herbal formula RCM-107 has demonstrated its inhibitory effects on lipid and carbohydrate absorption in our previous work. However, no published data described its effects on GLP-1 secretion. Therefore, this study aimed to determine the effects of RCM-107 and its individual ingredients on GLP-1 secretion via enzyme-linked immunosorbent assay (ELISA). Furthermore, molecular docking was performed to predict the key chemical compounds that are likely to be GLP-1 receptor agonists. <i>Gardeniae fructus</i>, one of the ingredients in RCM-107, demonstrated significantly greater effects on inducing GLP-1 secretion than the positive control epigallocatechin gallate (EGCG). Two <i>Gardeniae fructus</i> ligands, 3-epioleanolic acid and crocin were predicted to bind to the active form of GLP-1 receptor at the binding pocket with residues known for the receptor activation, suggesting that they could potentially serve as receptor agonists. Overall, this study reported the effects of researched herbs on GLP-1 secretion and proposed two compounds that may be responsible for antiobesity via GLP-1 receptor activation. |
| first_indexed | 2024-03-10T20:21:56Z |
| format | Article |
| id | doaj.art-1dcb0aee1d454f2d92919558224af142 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:21:56Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1dcb0aee1d454f2d92919558224af1422023-11-19T22:07:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218285410.3390/ijms21082854The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico StudyShiqi Luo0Harsharn Gill1Bryce Feltis2Andrew Hung3Linh Toan Nguyen4George Binh Lenon5School of Health and Biomedical Sciences, RMIT University, PO Box 71, Bundoora West Campus, Victoria 3083, AustraliaSchool of Science, RMIT University, GPO Box 2476, Melbourne, Victoria 3001, AustraliaSchool of Health and Biomedical Sciences, RMIT University, PO Box 71, Bundoora West Campus, Victoria 3083, AustraliaSchool of Science, RMIT University, GPO Box 2476, Melbourne, Victoria 3001, AustraliaDepartment of Endocrine, 103 Military Hospital, Vietnam Military Medical University, Hanoi 100000, VietnamSchool of Health and Biomedical Sciences, RMIT University, PO Box 71, Bundoora West Campus, Victoria 3083, AustraliaObesity is a multifactorial disease that can lead to other health issues. Glucagon-like peptide-1(GLP-1), as one of the satiety signal, has been linked with appetite suppression and weight loss. Due to the limitations of GLP-1 and its analogues, alternative treatments such as herbal therapies have become popular. The herbal formula RCM-107 has demonstrated its inhibitory effects on lipid and carbohydrate absorption in our previous work. However, no published data described its effects on GLP-1 secretion. Therefore, this study aimed to determine the effects of RCM-107 and its individual ingredients on GLP-1 secretion via enzyme-linked immunosorbent assay (ELISA). Furthermore, molecular docking was performed to predict the key chemical compounds that are likely to be GLP-1 receptor agonists. <i>Gardeniae fructus</i>, one of the ingredients in RCM-107, demonstrated significantly greater effects on inducing GLP-1 secretion than the positive control epigallocatechin gallate (EGCG). Two <i>Gardeniae fructus</i> ligands, 3-epioleanolic acid and crocin were predicted to bind to the active form of GLP-1 receptor at the binding pocket with residues known for the receptor activation, suggesting that they could potentially serve as receptor agonists. Overall, this study reported the effects of researched herbs on GLP-1 secretion and proposed two compounds that may be responsible for antiobesity via GLP-1 receptor activation.https://www.mdpi.com/1422-0067/21/8/2854GLP-1 secretionappetite suppressionobesityoverweightcell culturein silico analysis |
| spellingShingle | Shiqi Luo Harsharn Gill Bryce Feltis Andrew Hung Linh Toan Nguyen George Binh Lenon The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study International Journal of Molecular Sciences GLP-1 secretion appetite suppression obesity overweight cell culture in silico analysis |
| title | The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study |
| title_full | The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study |
| title_fullStr | The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study |
| title_full_unstemmed | The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study |
| title_short | The Effects of a Weight-Loss Herbal Formula RCM-107 and Its Eight Individual Ingredients on Glucagon-Like Peptide-1 Secretion—An In Vitro and In Silico Study |
| title_sort | effects of a weight loss herbal formula rcm 107 and its eight individual ingredients on glucagon like peptide 1 secretion an in vitro and in silico study |
| topic | GLP-1 secretion appetite suppression obesity overweight cell culture in silico analysis |
| url | https://www.mdpi.com/1422-0067/21/8/2854 |
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