Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture
(1) Background. An extensive survey of grapevine-sown cover crops and spontaneous weed flora was conducted from 2019 to 2020 in organic vineyards in six European countries (France, Italy, Romania, Slovenia, Spain, Switzerland). Our main objective was to detect and identify the presence of <i>C...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/6/498 |
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| author | Maela León Mónica Berbegal Paloma Abad-Campos Antonio Ramón-Albalat Tito Caffi Vittorio Rossi Gultakin Hasanaliyeva Pierre Antoine Noceto Daniel Wipf Saša Širca Jaka Razinger Anne-Laure Fragnière Patrik Kehrli Aurora Ranca Anamaria Petrescu Josep Armengol |
| author_facet | Maela León Mónica Berbegal Paloma Abad-Campos Antonio Ramón-Albalat Tito Caffi Vittorio Rossi Gultakin Hasanaliyeva Pierre Antoine Noceto Daniel Wipf Saša Širca Jaka Razinger Anne-Laure Fragnière Patrik Kehrli Aurora Ranca Anamaria Petrescu Josep Armengol |
| author_sort | Maela León |
| collection | DOAJ |
| description | (1) Background. An extensive survey of grapevine-sown cover crops and spontaneous weed flora was conducted from 2019 to 2020 in organic vineyards in six European countries (France, Italy, Romania, Slovenia, Spain, Switzerland). Our main objective was to detect and identify the presence of <i>Cylindrocarpon</i>-like asexual morphs species associated with black-foot disease on their roots. (2) Methods. Fungal isolations from root fragments were performed on culture media. <i>Cylindrocarpon</i>-like asexual morph species were identified by analyzing the DNA sequence data of the histone H3 (<i>his3</i>) gene region. In all, 685 plants belonging to different botanical families and genera were analyzed. <i>Cylindrocarpon</i>-like asexual morphs were recovered from 68 plants (9.9% of the total) and approximately 0.97% of the plated root fragments. (3) Results. Three fungal species (<i>Dactylonectria</i> <i>alcacerensis</i>, <i>Dactylonectria torresensis</i>, <i>Ilyonectria</i> <i>robusta</i>) were identified. <i>Dactylonectria</i> <i>torresensis</i> was the most frequent, and was isolated from many cover crop species in all six countries. A principal component analysis with the vineyard variables showed that seasonal temperatures and organic matter soil content correlated positively with <i>Cylindrocarpon</i>-like asexual morphs incidence. (4) Conclusions. The presence of <i>Cylindrocarpon</i>-like asexual morphs on roots of cover crops suggests that they can potentially act as alternative hosts for long-term survival or to increase inoculum levels in vineyard soils. |
| first_indexed | 2024-03-10T10:45:36Z |
| format | Article |
| id | doaj.art-1dcce517c25a4e98bb8d1b9d3dbf4a2f |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T10:45:36Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-1dcce517c25a4e98bb8d1b9d3dbf4a2f2023-11-21T22:37:35ZengMDPI AGBiology2079-77372021-06-0110649810.3390/biology10060498Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic ViticultureMaela León0Mónica Berbegal1Paloma Abad-Campos2Antonio Ramón-Albalat3Tito Caffi4Vittorio Rossi5Gultakin Hasanaliyeva6Pierre Antoine Noceto7Daniel Wipf8Saša Širca9Jaka Razinger10Anne-Laure Fragnière11Patrik Kehrli12Aurora Ranca13Anamaria Petrescu14Josep Armengol15Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, SpainInstituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, SpainInstituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, SpainInstituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, SpainDipartimento di Scienze delle Produzioni Vegetali Sostenibili, Università Cattolica del Sacro Cuore, DIPROVES—Crop Protection Area, Via Emilia Parmense 84, 29122 Piacenza, ItalyDipartimento di Scienze delle Produzioni Vegetali Sostenibili, Università Cattolica del Sacro Cuore, DIPROVES—Crop Protection Area, Via Emilia Parmense 84, 29122 Piacenza, ItalyDipartimento di Scienze delle Produzioni Vegetali Sostenibili, Università Cattolica del Sacro Cuore, DIPROVES—Crop Protection Area, Via Emilia Parmense 84, 29122 Piacenza, ItalyAgroécologie, AgroSup Dijon, CNRS, INRAE, Pôle IPM-ERL CNRS 6300, BP 86510, 17 rue Sully, CEDEX, 21065 Dijon, FranceAgroécologie, AgroSup Dijon, CNRS, INRAE, Pôle IPM-ERL CNRS 6300, BP 86510, 17 rue Sully, CEDEX, 21065 Dijon, FrancePlant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1000 Ljubljana, SloveniaPlant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1000 Ljubljana, SloveniaAgroscope, Route de Duillier 50, P.O. Box 1012, 1260 Nyon 1, SwitzerlandAgroscope, Route de Duillier 50, P.O. Box 1012, 1260 Nyon 1, SwitzerlandCalea Bucuresti, No.2, Murfatlar, 905100 Constanta, RomaniaCalea Bucuresti, No.2, Murfatlar, 905100 Constanta, RomaniaInstituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain(1) Background. An extensive survey of grapevine-sown cover crops and spontaneous weed flora was conducted from 2019 to 2020 in organic vineyards in six European countries (France, Italy, Romania, Slovenia, Spain, Switzerland). Our main objective was to detect and identify the presence of <i>Cylindrocarpon</i>-like asexual morphs species associated with black-foot disease on their roots. (2) Methods. Fungal isolations from root fragments were performed on culture media. <i>Cylindrocarpon</i>-like asexual morph species were identified by analyzing the DNA sequence data of the histone H3 (<i>his3</i>) gene region. In all, 685 plants belonging to different botanical families and genera were analyzed. <i>Cylindrocarpon</i>-like asexual morphs were recovered from 68 plants (9.9% of the total) and approximately 0.97% of the plated root fragments. (3) Results. Three fungal species (<i>Dactylonectria</i> <i>alcacerensis</i>, <i>Dactylonectria torresensis</i>, <i>Ilyonectria</i> <i>robusta</i>) were identified. <i>Dactylonectria</i> <i>torresensis</i> was the most frequent, and was isolated from many cover crop species in all six countries. A principal component analysis with the vineyard variables showed that seasonal temperatures and organic matter soil content correlated positively with <i>Cylindrocarpon</i>-like asexual morphs incidence. (4) Conclusions. The presence of <i>Cylindrocarpon</i>-like asexual morphs on roots of cover crops suggests that they can potentially act as alternative hosts for long-term survival or to increase inoculum levels in vineyard soils.https://www.mdpi.com/2079-7737/10/6/498<i>Dactylonectria</i><i>Ilyonectria</i>soil-borne fungi |
| spellingShingle | Maela León Mónica Berbegal Paloma Abad-Campos Antonio Ramón-Albalat Tito Caffi Vittorio Rossi Gultakin Hasanaliyeva Pierre Antoine Noceto Daniel Wipf Saša Širca Jaka Razinger Anne-Laure Fragnière Patrik Kehrli Aurora Ranca Anamaria Petrescu Josep Armengol Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture Biology <i>Dactylonectria</i> <i>Ilyonectria</i> soil-borne fungi |
| title | Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture |
| title_full | Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture |
| title_fullStr | Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture |
| title_full_unstemmed | Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture |
| title_short | Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture |
| title_sort | evaluation of sown cover crops and spontaneous weed flora as a potential reservoir of black foot pathogens in organic viticulture |
| topic | <i>Dactylonectria</i> <i>Ilyonectria</i> soil-borne fungi |
| url | https://www.mdpi.com/2079-7737/10/6/498 |
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