A Method for Determining the Content of Glycoproteins in Biological Samples
The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectromet...
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| Format: | Article |
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MDPI AG
2016-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/12/1625 |
| _version_ | 1828483722002825216 |
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| author | Yang Gao Duoduo Xu Hongyue Li Xianling Yang Mingxing Wang Qipin Gao |
| author_facet | Yang Gao Duoduo Xu Hongyue Li Xianling Yang Mingxing Wang Qipin Gao |
| author_sort | Yang Gao |
| collection | DOAJ |
| description | The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs. |
| first_indexed | 2024-12-11T08:42:47Z |
| format | Article |
| id | doaj.art-1dd1443db3254f66af60e6f1affc611a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T08:42:47Z |
| publishDate | 2016-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1dd1443db3254f66af60e6f1affc611a2022-12-22T01:14:12ZengMDPI AGMolecules1420-30492016-11-012112162510.3390/molecules21121625molecules21121625A Method for Determining the Content of Glycoproteins in Biological SamplesYang Gao0Duoduo Xu1Hongyue Li2Xianling Yang3Mingxing Wang4Qipin Gao5Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, ChinaJilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, ChinaJilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, ChinaJilin Institute For Drug Control, Changchun 130033, Jilin, ChinaJilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, ChinaJilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, ChinaThe glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.http://www.mdpi.com/1420-3049/21/12/1625glycoproteiniodine substitutionICP-MSbiological samplequantification |
| spellingShingle | Yang Gao Duoduo Xu Hongyue Li Xianling Yang Mingxing Wang Qipin Gao A Method for Determining the Content of Glycoproteins in Biological Samples Molecules glycoprotein iodine substitution ICP-MS biological sample quantification |
| title | A Method for Determining the Content of Glycoproteins in Biological Samples |
| title_full | A Method for Determining the Content of Glycoproteins in Biological Samples |
| title_fullStr | A Method for Determining the Content of Glycoproteins in Biological Samples |
| title_full_unstemmed | A Method for Determining the Content of Glycoproteins in Biological Samples |
| title_short | A Method for Determining the Content of Glycoproteins in Biological Samples |
| title_sort | method for determining the content of glycoproteins in biological samples |
| topic | glycoprotein iodine substitution ICP-MS biological sample quantification |
| url | http://www.mdpi.com/1420-3049/21/12/1625 |
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