MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity
Single cell cytometry has demonstrated plausible immuno-heterogeneity of mesenchymal stem cells (MSCs) owing to their multivariate stromal origin. To contribute successfully to next-generation stem cell therapeutics, a deeper understanding of their cellular morphology and immunophenotype is importan...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-11-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0048 |
| _version_ | 1797633118142726144 |
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| author | Ayona Gupta Safia Kousar Shaik Lakshmi Balasubramanian Uttara Chakraborty |
| author_facet | Ayona Gupta Safia Kousar Shaik Lakshmi Balasubramanian Uttara Chakraborty |
| author_sort | Ayona Gupta |
| collection | DOAJ |
| description | Single cell cytometry has demonstrated plausible immuno-heterogeneity of mesenchymal stem cells (MSCs) owing to their multivariate stromal origin. To contribute successfully to next-generation stem cell therapeutics, a deeper understanding of their cellular morphology and immunophenotype is important. In this study, the authors describe MSCProfiler, an image analysis pipeline developed using CellProfiler software. This workflow can extract geometrical and texture features such as shape, size, eccentricity and entropy, along with intensity values of the surface markers from multiple single cell images obtained using imaging flow cytometry. This screening pipeline can be used to analyze geometrical and texture features of all types of MSCs across different passages hallmarked by enhanced feature extraction potential from brightfield and fluorescent images of the cells. |
| first_indexed | 2024-03-11T11:49:31Z |
| format | Article |
| id | doaj.art-1dd51ae6c7064279a66c7d303ba80578 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-11T11:49:31Z |
| publishDate | 2023-11-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-1dd51ae6c7064279a66c7d303ba805782023-11-09T10:05:27ZengFuture Science LtdBioTechniques0736-62051940-98182023-11-0175519520910.2144/btn-2023-0048MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneityAyona Gupta0Safia Kousar Shaik1Lakshmi Balasubramanian2Uttara Chakraborty31Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India2Delft University of Technology, Delft, The Netherlands3SaviTix Technology Consultancy, Bengaluru, India1Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, IndiaSingle cell cytometry has demonstrated plausible immuno-heterogeneity of mesenchymal stem cells (MSCs) owing to their multivariate stromal origin. To contribute successfully to next-generation stem cell therapeutics, a deeper understanding of their cellular morphology and immunophenotype is important. In this study, the authors describe MSCProfiler, an image analysis pipeline developed using CellProfiler software. This workflow can extract geometrical and texture features such as shape, size, eccentricity and entropy, along with intensity values of the surface markers from multiple single cell images obtained using imaging flow cytometry. This screening pipeline can be used to analyze geometrical and texture features of all types of MSCs across different passages hallmarked by enhanced feature extraction potential from brightfield and fluorescent images of the cells.https://www.future-science.com/doi/10.2144/btn-2023-0048biomarkersCellProfilerimage data analysisimaging flow cytometryimmuno-heterogeneitymesenchymal stem cells |
| spellingShingle | Ayona Gupta Safia Kousar Shaik Lakshmi Balasubramanian Uttara Chakraborty MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity BioTechniques biomarkers CellProfiler image data analysis imaging flow cytometry immuno-heterogeneity mesenchymal stem cells |
| title | MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| title_full | MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| title_fullStr | MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| title_full_unstemmed | MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| title_short | MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| title_sort | mscprofiler a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity |
| topic | biomarkers CellProfiler image data analysis imaging flow cytometry immuno-heterogeneity mesenchymal stem cells |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0048 |
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