Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natur...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2021-04-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/11215.pdf |
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| author | Claudia-Anahí Pérez-Torres Enrique Ibarra-Laclette Eric-Edmundo Hernández-Domínguez Benjamín Rodríguez-Haas Alan-Josué Pérez-Lira Emanuel Villafán Alexandro Alonso-Sánchez Clemente de Jesús García-Ávila José-Abrahán Ramírez-Pool Diana Sánchez-Rangel |
| author_facet | Claudia-Anahí Pérez-Torres Enrique Ibarra-Laclette Eric-Edmundo Hernández-Domínguez Benjamín Rodríguez-Haas Alan-Josué Pérez-Lira Emanuel Villafán Alexandro Alonso-Sánchez Clemente de Jesús García-Ávila José-Abrahán Ramírez-Pool Diana Sánchez-Rangel |
| author_sort | Claudia-Anahí Pérez-Torres |
| collection | DOAJ |
| description | Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natural forests, landscape trees, and avocado orchards in the last 8 years. Despite the interest in this species, the molecular responses of both the host and F. kuroshium during the infection process and disease establishment remain unknown. In this work, we established an in vitro pathosystem using Hass avocado stems inoculated with F. kuroshium to investigate differential gene expression at 1, 4, 7 and 14 days post-inoculation. RNA-seq technology allowed us to obtain data from both the plant and the fungus, and the sequences obtained from both organisms were analyzed independently. The pathosystem established was able to mimic Fusarium dieback symptoms, such as carbohydrate exudation, necrosis, and vascular tissue discoloration. The results provide interesting evidence regarding the genes that may play roles in the avocado defense response to Fusarium dieback disease. The avocado data set comprised a coding sequence collection of 51,379 UniGenes, from which 2,403 (4.67%) were identified as differentially expressed. The global expression analysis showed that F. kuroshium responsive UniGenes can be clustered into six groups according to their expression profiles. The biologically relevant functional categories that were identified included photosynthesis as well as responses to stress, hormones, abscisic acid, and water deprivation. Additionally, processes such as oxidation-reduction, organization and biogenesis of the cell wall and polysaccharide metabolism were detected. Moreover, we identified orthologues of nucleotide-binding leucine-rich receptors, and their possible action mode was analyzed. In F. kuroshium, we identified 57 differentially expressed genes. Interestingly, the alcohol metabolic process biological category had the highest number of upregulated genes, and the enzyme group in this category may play an important role in the mechanisms of secondary metabolite detoxification. Hydrolytic enzymes, such as endoglucanases and a pectate lyase, were also identified, as well as some proteases. In conclusion, our research was conducted mainly to explain how the vascular tissue of a recognized host of the ambrosia complex responds during F. kuroshium infection since Fusarium dieback is an ambrosia beetle-vectored disease and many variables facilitate its establishment. |
| first_indexed | 2024-03-09T08:01:24Z |
| format | Article |
| id | doaj.art-1dd5343b934a4a5c954b9388119375a2 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:01:24Z |
| publishDate | 2021-04-01 |
| publisher | PeerJ Inc. |
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| series | PeerJ |
| spelling | doaj.art-1dd5343b934a4a5c954b9388119375a22023-12-03T00:41:45ZengPeerJ Inc.PeerJ2167-83592021-04-019e1121510.7717/peerj.11215Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-SeqClaudia-Anahí Pérez-Torres0Enrique Ibarra-Laclette1Eric-Edmundo Hernández-Domínguez2Benjamín Rodríguez-Haas3Alan-Josué Pérez-Lira4Emanuel Villafán5Alexandro Alonso-Sánchez6Clemente de Jesús García-Ávila7José-Abrahán Ramírez-Pool8Diana Sánchez-Rangel9Catedrático CONACyT en la Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoCatedrático CONACyT en la Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoCentro Nacional de Referencia Fitosanitaria, Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria, Tecámac, Estado de México, MéxicoCentro Nacional de Referencia Fitosanitaria, Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria, Tecámac, Estado de México, MéxicoCatedrático CONACyT en la Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa, Veracruz, MéxicoFusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natural forests, landscape trees, and avocado orchards in the last 8 years. Despite the interest in this species, the molecular responses of both the host and F. kuroshium during the infection process and disease establishment remain unknown. In this work, we established an in vitro pathosystem using Hass avocado stems inoculated with F. kuroshium to investigate differential gene expression at 1, 4, 7 and 14 days post-inoculation. RNA-seq technology allowed us to obtain data from both the plant and the fungus, and the sequences obtained from both organisms were analyzed independently. The pathosystem established was able to mimic Fusarium dieback symptoms, such as carbohydrate exudation, necrosis, and vascular tissue discoloration. The results provide interesting evidence regarding the genes that may play roles in the avocado defense response to Fusarium dieback disease. The avocado data set comprised a coding sequence collection of 51,379 UniGenes, from which 2,403 (4.67%) were identified as differentially expressed. The global expression analysis showed that F. kuroshium responsive UniGenes can be clustered into six groups according to their expression profiles. The biologically relevant functional categories that were identified included photosynthesis as well as responses to stress, hormones, abscisic acid, and water deprivation. Additionally, processes such as oxidation-reduction, organization and biogenesis of the cell wall and polysaccharide metabolism were detected. Moreover, we identified orthologues of nucleotide-binding leucine-rich receptors, and their possible action mode was analyzed. In F. kuroshium, we identified 57 differentially expressed genes. Interestingly, the alcohol metabolic process biological category had the highest number of upregulated genes, and the enzyme group in this category may play an important role in the mechanisms of secondary metabolite detoxification. Hydrolytic enzymes, such as endoglucanases and a pectate lyase, were also identified, as well as some proteases. In conclusion, our research was conducted mainly to explain how the vascular tissue of a recognized host of the ambrosia complex responds during F. kuroshium infection since Fusarium dieback is an ambrosia beetle-vectored disease and many variables facilitate its establishment.https://peerj.com/articles/11215.pdfFusarium kuroshiumAvocado stemsFusarium diebackFungal alcohol metabolismNucleotide-binding leucine-rich receptors (NLRs) |
| spellingShingle | Claudia-Anahí Pérez-Torres Enrique Ibarra-Laclette Eric-Edmundo Hernández-Domínguez Benjamín Rodríguez-Haas Alan-Josué Pérez-Lira Emanuel Villafán Alexandro Alonso-Sánchez Clemente de Jesús García-Ávila José-Abrahán Ramírez-Pool Diana Sánchez-Rangel Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq PeerJ Fusarium kuroshium Avocado stems Fusarium dieback Fungal alcohol metabolism Nucleotide-binding leucine-rich receptors (NLRs) |
| title | Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq |
| title_full | Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq |
| title_fullStr | Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq |
| title_full_unstemmed | Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq |
| title_short | Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq |
| title_sort | molecular evidence of the avocado defense response to fusarium kuroshium infection a deep transcriptome analysis using rna seq |
| topic | Fusarium kuroshium Avocado stems Fusarium dieback Fungal alcohol metabolism Nucleotide-binding leucine-rich receptors (NLRs) |
| url | https://peerj.com/articles/11215.pdf |
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