Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae)
A comparative karyotype analysis of four species of yellow-flowered <i>Eranthis</i> sect. <i>Eranthis</i>, i.e., <i>E. bulgarica</i>, <i>E. cilicica</i>, <i>E. hyemalis</i>, and <i>E. longistipitata</i> from different areas, has...
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MDPI AG
2023-12-01
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| author | Elizaveta Yu. Mitrenina Svetlana S. Alekseeva Ekaterina D. Badaeva Lorenzo Peruzzi Gleb N. Artemov Denis A. Krivenko Lorenzo Pinzani Zeki Aytaç Ömer Çeçen Shukherdorj Baasanmunkh Hyeok Jae Choi Attila Mesterházy Alexander N. Tashev Svetlana Bancheva Lian Lian Kunli Xiang Wei Wang Andrey S. Erst |
| author_facet | Elizaveta Yu. Mitrenina Svetlana S. Alekseeva Ekaterina D. Badaeva Lorenzo Peruzzi Gleb N. Artemov Denis A. Krivenko Lorenzo Pinzani Zeki Aytaç Ömer Çeçen Shukherdorj Baasanmunkh Hyeok Jae Choi Attila Mesterházy Alexander N. Tashev Svetlana Bancheva Lian Lian Kunli Xiang Wei Wang Andrey S. Erst |
| author_sort | Elizaveta Yu. Mitrenina |
| collection | DOAJ |
| description | A comparative karyotype analysis of four species of yellow-flowered <i>Eranthis</i> sect. <i>Eranthis</i>, i.e., <i>E. bulgarica</i>, <i>E. cilicica</i>, <i>E. hyemalis</i>, and <i>E. longistipitata</i> from different areas, has been carried out for the first time. All the studied specimens had somatic chromosome number 2<i>n</i> = 16 with basic chromosome number <i>x</i> = 8. Karyotypes of the investigated plants included five pairs of metacentric chromosomes and three pairs of submetacentric/subtelocentric chromosomes. The chromosome sets of the investigated species differ mainly in the ratio of submetacentric/subtelocentric chromosomes, their relative lengths, and arm ratios. A new oligonucleotide probe was developed and tested to detect 45S rDNA clusters. Using this probe and an oligonucleotide probe to 5S rDNA, 45S and 5S rDNA clusters were localized for the first time on chromosomes of <i>E. cilicica</i>, <i>E. hyemalis</i>, and <i>E. longistipitata</i>. Major 45S rDNA clusters were identified on satellite chromosomes in all the species; in <i>E. cilicica</i>, minor clusters were also identified in the terminal regions of one metacentric chromosome pair. The number and distribution of 5S rDNA clusters is more specific. In <i>E. cilicica</i>, two major clusters were identified in the pericentromeric region of a pair of metacentric chromosomes. Two major clusters in the pericentromeric region of a pair of submetacentric chromosomes and two major clusters in the interstitial region of a pair of metacentric chromosomes were observed in <i>E. longistipitata</i>. <i>E. hyemalis</i> has many clusters of different sizes, localized mainly in the pericentromeric regions. Summarizing new data on the karyotype structure of <i>E.</i> sect. <i>Eranthis</i> and previously obtained data on <i>E.</i> sect. <i>Shibateranthis</i> allowed conclusions to be formed about the clear interspecific karyological differences of the genus <i>Eranthis</i>. |
| first_indexed | 2024-03-08T14:59:02Z |
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| publishDate | 2023-12-01 |
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| series | Plants |
| spelling | doaj.art-1dd8926a1b5147908bb0c36bad8eb5862024-01-10T15:06:04ZengMDPI AGPlants2223-77472023-12-011314710.3390/plants13010047Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae)Elizaveta Yu. Mitrenina0Svetlana S. Alekseeva1Ekaterina D. Badaeva2Lorenzo Peruzzi3Gleb N. Artemov4Denis A. Krivenko5Lorenzo Pinzani6Zeki Aytaç7Ömer Çeçen8Shukherdorj Baasanmunkh9Hyeok Jae Choi10Attila Mesterházy11Alexander N. Tashev12Svetlana Bancheva13Lian Lian14Kunli Xiang15Wei Wang16Andrey S. Erst17Department of Genetics and Cell Biology, Biological Institute, National Research Tomsk State University, 634050 Tomsk, RussiaDepartment of Genetics and Cell Biology, Biological Institute, National Research Tomsk State University, 634050 Tomsk, RussiaVavilov Institute of General Genetics, Russian Academy of Sciences, 119333 Moscow, RussiaPLANTSEED Lab, Department of Biology, University of Pisa, 56126 Pisa, ItalyDepartment of Genetics and Cell Biology, Biological Institute, National Research Tomsk State University, 634050 Tomsk, RussiaCentral Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaPLANTSEED Lab, Department of Biology, University of Pisa, 56126 Pisa, ItalyBiology Department, Faculty of Science, Gazi University, Ankara 06500, TurkeyDepartment of Plant and Animal Production, Technical Sciences Vocational School, Karamanoğlu Mehmetbey University, Karaman 70100, TurkeyDepartment of Biology and Chemistry, Changwon National University, Changwon 51140, Republic of KoreaDepartment of Biology and Chemistry, Changwon National University, Changwon 51140, Republic of KoreaIndependent Researcher, H-9500 Celldömölk, HungaryDepartment of Dendrology, University of Forestry, 1756 Sofia, BulgariaBotanical Garden, Bulgarian Academy of Sciences, 1616 Sofia, BulgariaState Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaState Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaState Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaCentral Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaA comparative karyotype analysis of four species of yellow-flowered <i>Eranthis</i> sect. <i>Eranthis</i>, i.e., <i>E. bulgarica</i>, <i>E. cilicica</i>, <i>E. hyemalis</i>, and <i>E. longistipitata</i> from different areas, has been carried out for the first time. All the studied specimens had somatic chromosome number 2<i>n</i> = 16 with basic chromosome number <i>x</i> = 8. Karyotypes of the investigated plants included five pairs of metacentric chromosomes and three pairs of submetacentric/subtelocentric chromosomes. The chromosome sets of the investigated species differ mainly in the ratio of submetacentric/subtelocentric chromosomes, their relative lengths, and arm ratios. A new oligonucleotide probe was developed and tested to detect 45S rDNA clusters. Using this probe and an oligonucleotide probe to 5S rDNA, 45S and 5S rDNA clusters were localized for the first time on chromosomes of <i>E. cilicica</i>, <i>E. hyemalis</i>, and <i>E. longistipitata</i>. Major 45S rDNA clusters were identified on satellite chromosomes in all the species; in <i>E. cilicica</i>, minor clusters were also identified in the terminal regions of one metacentric chromosome pair. The number and distribution of 5S rDNA clusters is more specific. In <i>E. cilicica</i>, two major clusters were identified in the pericentromeric region of a pair of metacentric chromosomes. Two major clusters in the pericentromeric region of a pair of submetacentric chromosomes and two major clusters in the interstitial region of a pair of metacentric chromosomes were observed in <i>E. longistipitata</i>. <i>E. hyemalis</i> has many clusters of different sizes, localized mainly in the pericentromeric regions. Summarizing new data on the karyotype structure of <i>E.</i> sect. <i>Eranthis</i> and previously obtained data on <i>E.</i> sect. <i>Shibateranthis</i> allowed conclusions to be formed about the clear interspecific karyological differences of the genus <i>Eranthis</i>.https://www.mdpi.com/2223-7747/13/1/47<i>Eranthis</i>chromosomesfluorescence in situ hybridization (FISH)karyotypeoligonucleotide probesRanunculaceae |
| spellingShingle | Elizaveta Yu. Mitrenina Svetlana S. Alekseeva Ekaterina D. Badaeva Lorenzo Peruzzi Gleb N. Artemov Denis A. Krivenko Lorenzo Pinzani Zeki Aytaç Ömer Çeçen Shukherdorj Baasanmunkh Hyeok Jae Choi Attila Mesterházy Alexander N. Tashev Svetlana Bancheva Lian Lian Kunli Xiang Wei Wang Andrey S. Erst Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) Plants <i>Eranthis</i> chromosomes fluorescence in situ hybridization (FISH) karyotype oligonucleotide probes Ranunculaceae |
| title | Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) |
| title_full | Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) |
| title_fullStr | Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) |
| title_full_unstemmed | Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) |
| title_short | Karyotypes and Physical Mapping of Ribosomal DNA with Oligo-Probes in <i>Eranthis</i> sect. <i>Eranthis</i> (Ranunculaceae) |
| title_sort | karyotypes and physical mapping of ribosomal dna with oligo probes in i eranthis i sect i eranthis i ranunculaceae |
| topic | <i>Eranthis</i> chromosomes fluorescence in situ hybridization (FISH) karyotype oligonucleotide probes Ranunculaceae |
| url | https://www.mdpi.com/2223-7747/13/1/47 |
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