Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics

Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics

Quantifying gene expression in individual cells can substantially improve our understanding about complex genetically engineered cell products such as chimeric antigen receptor (CAR) T cells. Here we designed a single-cell RNA sequencing (scRNA-seq) approach to monitor the delivery of a CD19-CAR gen...

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Bibliographic Details
Main Authors: Filippos T. Charitidis, Elham Adabi, Frederic B. Thalheimer, Colin Clarke, Christian J. Buchholz
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
CAR T cells
CAR
chimeric antigen receptor
CD19
lentiviral vector
scRNA sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050121001571

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