Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells?
Numerous intervention strategies have been developed to promote functional tissue repair following experimental spinal cord injury (SCI), including the bridging of lesion-induced cystic cavities with bioengineered scaffolds. Integration between such implanted scaffolds and the lesioned host spinal c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3221 |
| _version_ | 1797470806357311488 |
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| author | Haktan Altinova Pascal Achenbach Moniek Palm Istvan Katona Emmanuel Hermans Hans Clusmann Joachim Weis Gary Anthony Brook |
| author_facet | Haktan Altinova Pascal Achenbach Moniek Palm Istvan Katona Emmanuel Hermans Hans Clusmann Joachim Weis Gary Anthony Brook |
| author_sort | Haktan Altinova |
| collection | DOAJ |
| description | Numerous intervention strategies have been developed to promote functional tissue repair following experimental spinal cord injury (SCI), including the bridging of lesion-induced cystic cavities with bioengineered scaffolds. Integration between such implanted scaffolds and the lesioned host spinal cord is critical for supporting regenerative growth, but only moderate-to-low degrees of success have been reported. Light and electron microscopy were employed to better characterise the fibroadhesive scarring process taking place after implantation of a longitudinally microstructured type-I collagen scaffold into unilateral mid-cervical resection injuries of the adult rat spinal cord. At long survival times (10 weeks post-surgery), sheets of tightly packed cells (of uniform morphology) could be seen lining the inner surface of the repaired dura mater of lesion-only control animals, as well as forming a barrier along the implant–host interface of the scaffold-implanted animals. The highly uniform ultrastructural features of these scarring cells and their anatomical continuity with the local, reactive spinal nerve roots strongly suggest their identity to be perineurial-like cells. This novel aspect of the cellular composition of reactive spinal cord tissue highlights the increasingly complex nature of fibroadhesive scarring involved in traumatic injury, and particularly in response to the implantation of bioengineered collagen scaffolds. |
| first_indexed | 2024-03-09T19:42:07Z |
| format | Article |
| id | doaj.art-1ddb78544e1c44d2a057d6e50fde3221 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:42:07Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1ddb78544e1c44d2a057d6e50fde32212023-11-24T01:35:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236322110.3390/ijms23063221Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells?Haktan Altinova0Pascal Achenbach1Moniek Palm2Istvan Katona3Emmanuel Hermans4Hans Clusmann5Joachim Weis6Gary Anthony Brook7Institute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuroscience, Université Catholique de Louvain, 1200 Brussels, BelgiumDepartment of Neurosurgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Neuropathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyNumerous intervention strategies have been developed to promote functional tissue repair following experimental spinal cord injury (SCI), including the bridging of lesion-induced cystic cavities with bioengineered scaffolds. Integration between such implanted scaffolds and the lesioned host spinal cord is critical for supporting regenerative growth, but only moderate-to-low degrees of success have been reported. Light and electron microscopy were employed to better characterise the fibroadhesive scarring process taking place after implantation of a longitudinally microstructured type-I collagen scaffold into unilateral mid-cervical resection injuries of the adult rat spinal cord. At long survival times (10 weeks post-surgery), sheets of tightly packed cells (of uniform morphology) could be seen lining the inner surface of the repaired dura mater of lesion-only control animals, as well as forming a barrier along the implant–host interface of the scaffold-implanted animals. The highly uniform ultrastructural features of these scarring cells and their anatomical continuity with the local, reactive spinal nerve roots strongly suggest their identity to be perineurial-like cells. This novel aspect of the cellular composition of reactive spinal cord tissue highlights the increasingly complex nature of fibroadhesive scarring involved in traumatic injury, and particularly in response to the implantation of bioengineered collagen scaffolds.https://www.mdpi.com/1422-0067/23/6/3221spinal cord injurymicrostructured collagen scaffoldCNS-scarringperineurial-like cellsfibrotic encapsulationimplant interface |
| spellingShingle | Haktan Altinova Pascal Achenbach Moniek Palm Istvan Katona Emmanuel Hermans Hans Clusmann Joachim Weis Gary Anthony Brook Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? International Journal of Molecular Sciences spinal cord injury microstructured collagen scaffold CNS-scarring perineurial-like cells fibrotic encapsulation implant interface |
| title | Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? |
| title_full | Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? |
| title_fullStr | Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? |
| title_full_unstemmed | Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? |
| title_short | Characterization of a Novel Aspect of Tissue Scarring Following Experimental Spinal Cord Injury and the Implantation of Bioengineered Type-I Collagen Scaffolds in the Adult Rat: Involvement of Perineurial-like Cells? |
| title_sort | characterization of a novel aspect of tissue scarring following experimental spinal cord injury and the implantation of bioengineered type i collagen scaffolds in the adult rat involvement of perineurial like cells |
| topic | spinal cord injury microstructured collagen scaffold CNS-scarring perineurial-like cells fibrotic encapsulation implant interface |
| url | https://www.mdpi.com/1422-0067/23/6/3221 |
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