Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells
Various chronic inflammatory diseases have become a problem, especially in the Western world. Whether it concerns inflammation of visceral organs, joints, bones, etc., it is always a physiological reaction of the body, which always tries to eradicate harmful substances and restore tissue homeostasis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Chemistry Proceedings |
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| Online Access: | https://www.mdpi.com/2673-4583/8/1/108 |
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| author | Josef Jampilek Jan Hosek Pavel Bobal |
| author_facet | Josef Jampilek Jan Hosek Pavel Bobal |
| author_sort | Josef Jampilek |
| collection | DOAJ |
| description | Various chronic inflammatory diseases have become a problem, especially in the Western world. Whether it concerns inflammation of visceral organs, joints, bones, etc., it is always a physiological reaction of the body, which always tries to eradicate harmful substances and restore tissue homeostasis. Unfortunately, prolonged or chronic inflammation often results in damage to the affected tissues. Diseases such as osteoarthritis, rheumatoid arthritis, and arthrosis, as well as cartilage damage, are very common. In addition to suppressing inflammation in the joints and around the cartilage, it is advantageous to administer compounds that are capable of stimulating cartilage growth and regenerating damaged tissue. Variously substituted piperazine-2,5-dione derivatives were investigated as compounds with a potential effect on cartilage regeneration. A series of assays were performed to evaluate their cytotoxicity, anti-inflammatory activity, and ability to potentiate chondrocyte proliferation and suppress synovial cell growth. The compounds proved to be completely non-toxic for all used types of cells up to the concentration of 20 µM. Unfortunately, their evaluated biological activity proved to be insignificant based on the comparison with untreated cells. |
| first_indexed | 2024-03-11T06:45:25Z |
| format | Article |
| id | doaj.art-1ddf7d0b5e4d4c90887ff2f11df5e0c3 |
| institution | Directory Open Access Journal |
| issn | 2673-4583 |
| language | English |
| last_indexed | 2024-03-11T06:45:25Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemistry Proceedings |
| spelling | doaj.art-1ddf7d0b5e4d4c90887ff2f11df5e0c32023-11-17T10:19:34ZengMDPI AGChemistry Proceedings2673-45832021-11-018110810.3390/ecsoc-25-11650Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related CellsJosef Jampilek0Jan Hosek1Pavel Bobal2Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, 842 15 Bratislava, SlovakiaDepartment of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicDepartment of Chemical Drugs, Faculty of Pharmacy, Masaryk University, Palackeho 1946/1, 612 00 Brno, Czech RepublicVarious chronic inflammatory diseases have become a problem, especially in the Western world. Whether it concerns inflammation of visceral organs, joints, bones, etc., it is always a physiological reaction of the body, which always tries to eradicate harmful substances and restore tissue homeostasis. Unfortunately, prolonged or chronic inflammation often results in damage to the affected tissues. Diseases such as osteoarthritis, rheumatoid arthritis, and arthrosis, as well as cartilage damage, are very common. In addition to suppressing inflammation in the joints and around the cartilage, it is advantageous to administer compounds that are capable of stimulating cartilage growth and regenerating damaged tissue. Variously substituted piperazine-2,5-dione derivatives were investigated as compounds with a potential effect on cartilage regeneration. A series of assays were performed to evaluate their cytotoxicity, anti-inflammatory activity, and ability to potentiate chondrocyte proliferation and suppress synovial cell growth. The compounds proved to be completely non-toxic for all used types of cells up to the concentration of 20 µM. Unfortunately, their evaluated biological activity proved to be insignificant based on the comparison with untreated cells.https://www.mdpi.com/2673-4583/8/1/108piperazine-2,5-dionescytotoxicityviability assayanti-inflammatory activitychondrocytessynovial cells |
| spellingShingle | Josef Jampilek Jan Hosek Pavel Bobal Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells Chemistry Proceedings piperazine-2,5-diones cytotoxicity viability assay anti-inflammatory activity chondrocytes synovial cells |
| title | Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells |
| title_full | Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells |
| title_fullStr | Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells |
| title_full_unstemmed | Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells |
| title_short | Investigation of the Effect of Selected Piperazine-2,5-Diones on Cartilage-Related Cells |
| title_sort | investigation of the effect of selected piperazine 2 5 diones on cartilage related cells |
| topic | piperazine-2,5-diones cytotoxicity viability assay anti-inflammatory activity chondrocytes synovial cells |
| url | https://www.mdpi.com/2673-4583/8/1/108 |
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