Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects
Abstract Extracellular vesicles (EVs) are lipid-bilayered particles, containing various biomolecules, including nucleic acids, lipids, and proteins, released by cells from all the domains of life and performing multiple communication functions. Evidence suggests that the interaction between host imm...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51370-7 |
| _version_ | 1827388456881356800 |
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| author | Stefano Nenciarini Roberta Amoriello Giovanni Bacci Benedetta Cerasuolo Monica Di Paola Patrizia Nardini Alessio Papini Clara Ballerini Duccio Cavalieri |
| author_facet | Stefano Nenciarini Roberta Amoriello Giovanni Bacci Benedetta Cerasuolo Monica Di Paola Patrizia Nardini Alessio Papini Clara Ballerini Duccio Cavalieri |
| author_sort | Stefano Nenciarini |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are lipid-bilayered particles, containing various biomolecules, including nucleic acids, lipids, and proteins, released by cells from all the domains of life and performing multiple communication functions. Evidence suggests that the interaction between host immune cells and fungal EVs induces modulation of the immune system. Most of the studies on fungal EVs have been conducted in the context of fungal infections; therefore, there is a knowledge gap in what concerns the production of EVs by yeasts in other contexts rather than infection and that may affect human health. In this work, we characterized EVs obtained by Saccharomyces cerevisiae and Pichia fermentans strains isolated from a fermented milk product with probiotic properties. The immunomodulation abilities of EVs produced by these strains have been studied in vitro through immune assays after internalization from human monocyte-derived dendritic cells. Results showed a significant reduction in antigen presentation activity of dendritic cells treated with the fermented milk EVs. The small RNA fraction of EVs contained mainly yeast mRNA sequences, with a few molecular functions enriched in strains of two different species isolated from the fermented milk. Our results suggest that one of the mechanisms behind the anti-inflammatory properties of probiotic foods could be mediated by the interactions of human immune cells with yeast EVs. |
| first_indexed | 2024-03-08T16:20:08Z |
| format | Article |
| id | doaj.art-1ddffc1bde2848b69a2a366a779510e1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:20:08Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1ddffc1bde2848b69a2a366a779510e12024-01-07T12:24:53ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-024-51370-7Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effectsStefano Nenciarini0Roberta Amoriello1Giovanni Bacci2Benedetta Cerasuolo3Monica Di Paola4Patrizia Nardini5Alessio Papini6Clara Ballerini7Duccio Cavalieri8Department of Biology, University of FlorenceDepartment of Experimental and Clinical Medicine, University of FlorenceDepartment of Biology, University of FlorenceDepartment of Biology, University of FlorenceDepartment of Biology, University of FlorenceDepartment of Experimental and Clinical Medicine, University of FlorenceDepartment of Biology, University of FlorenceDepartment of Experimental and Clinical Medicine, University of FlorenceDepartment of Biology, University of FlorenceAbstract Extracellular vesicles (EVs) are lipid-bilayered particles, containing various biomolecules, including nucleic acids, lipids, and proteins, released by cells from all the domains of life and performing multiple communication functions. Evidence suggests that the interaction between host immune cells and fungal EVs induces modulation of the immune system. Most of the studies on fungal EVs have been conducted in the context of fungal infections; therefore, there is a knowledge gap in what concerns the production of EVs by yeasts in other contexts rather than infection and that may affect human health. In this work, we characterized EVs obtained by Saccharomyces cerevisiae and Pichia fermentans strains isolated from a fermented milk product with probiotic properties. The immunomodulation abilities of EVs produced by these strains have been studied in vitro through immune assays after internalization from human monocyte-derived dendritic cells. Results showed a significant reduction in antigen presentation activity of dendritic cells treated with the fermented milk EVs. The small RNA fraction of EVs contained mainly yeast mRNA sequences, with a few molecular functions enriched in strains of two different species isolated from the fermented milk. Our results suggest that one of the mechanisms behind the anti-inflammatory properties of probiotic foods could be mediated by the interactions of human immune cells with yeast EVs.https://doi.org/10.1038/s41598-024-51370-7 |
| spellingShingle | Stefano Nenciarini Roberta Amoriello Giovanni Bacci Benedetta Cerasuolo Monica Di Paola Patrizia Nardini Alessio Papini Clara Ballerini Duccio Cavalieri Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects Scientific Reports |
| title | Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects |
| title_full | Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects |
| title_fullStr | Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects |
| title_full_unstemmed | Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects |
| title_short | Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects |
| title_sort | yeast strains isolated from fermented beverage produce extracellular vesicles with anti inflammatory effects |
| url | https://doi.org/10.1038/s41598-024-51370-7 |
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