Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards
An UPLC Quadrupole Orbitrap high resolution mass Spectrometry method for accurate quantification of aflatoxins in grains and oils was established, which were purified by automatic immunoaffinity column. The sample was extracted with 84/16(V/V) acetonitrile/water and centrifuged. The supernatant was...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2023-09-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230526 |
| _version_ | 1797659560189624320 |
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| author | YE Jin LIU Tong-tong LUO Li-juan WU Yu LI Li WANG Song-xue |
| author_facet | YE Jin LIU Tong-tong LUO Li-juan WU Yu LI Li WANG Song-xue |
| author_sort | YE Jin |
| collection | DOAJ |
| description | An UPLC Quadrupole Orbitrap high resolution mass Spectrometry method for accurate quantification of aflatoxins in grains and oils was established, which were purified by automatic immunoaffinity column. The sample was extracted with 84/16(V/V) acetonitrile/water and centrifuged. The supernatant was diluted 10 times with 0.1% PBST. Aflatoxin immunoaffinity column without background and recovery greater than 90% was chosen for purification automatically. UPLC-Quadrupole Orbitrap HRMS was used for quantitative analysis. Results showed that recoveries for aflatoxins were 84.7%~104.6%. The intra-day relative standard deviations were 1.7%~9.4%, and the intra-day precision were 0.7%~8.4%. The developed method can minimize the workload, improve work efficiency and avoid the result deviation caused by operation manually. It is suitable for the accurate determination of aflatoxins in grains and oils, and meet the requirement of the daily test and monitoring aflatoxins. |
| first_indexed | 2024-03-11T18:16:55Z |
| format | Article |
| id | doaj.art-1de50ff3c6de40ff84caf71d219f848b |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-03-11T18:16:55Z |
| publishDate | 2023-09-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-1de50ff3c6de40ff84caf71d219f848b2023-10-16T05:46:01ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612023-09-0131517017810.16210/j.cnki.1007-7561.2023.05.020Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal StandardsYE Jin0LIU Tong-tong1LUO Li-juan2WU Yu3LI Li4WANG Song-xue5Institute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaSchool of Health Science Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaAn UPLC Quadrupole Orbitrap high resolution mass Spectrometry method for accurate quantification of aflatoxins in grains and oils was established, which were purified by automatic immunoaffinity column. The sample was extracted with 84/16(V/V) acetonitrile/water and centrifuged. The supernatant was diluted 10 times with 0.1% PBST. Aflatoxin immunoaffinity column without background and recovery greater than 90% was chosen for purification automatically. UPLC-Quadrupole Orbitrap HRMS was used for quantitative analysis. Results showed that recoveries for aflatoxins were 84.7%~104.6%. The intra-day relative standard deviations were 1.7%~9.4%, and the intra-day precision were 0.7%~8.4%. The developed method can minimize the workload, improve work efficiency and avoid the result deviation caused by operation manually. It is suitable for the accurate determination of aflatoxins in grains and oils, and meet the requirement of the daily test and monitoring aflatoxins.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230526automatic solid phase extraction instrumentultra-high performance liquid chromatography- quadrupole orbitrap high-resolution mass spectrometricaflatoxinimmunoaffinity columnfully 13c isotope labeled internal standard calibration method |
| spellingShingle | YE Jin LIU Tong-tong LUO Li-juan WU Yu LI Li WANG Song-xue Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards Liang you shipin ke-ji automatic solid phase extraction instrument ultra-high performance liquid chromatography- quadrupole orbitrap high-resolution mass spectrometric aflatoxin immunoaffinity column fully 13c isotope labeled internal standard calibration method |
| title | Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards |
| title_full | Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards |
| title_fullStr | Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards |
| title_full_unstemmed | Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards |
| title_short | Accurate Determination of Aflatoxin in Grains and Oils use Liquid Chromatography Mass Spectrometry Purified by Automatic Solid Phase Extraction Instrument and Quantified by Fully 13C-Labelled Internal Standards |
| title_sort | accurate determination of aflatoxin in grains and oils use liquid chromatography mass spectrometry purified by automatic solid phase extraction instrument and quantified by fully 13c labelled internal standards |
| topic | automatic solid phase extraction instrument ultra-high performance liquid chromatography- quadrupole orbitrap high-resolution mass spectrometric aflatoxin immunoaffinity column fully 13c isotope labeled internal standard calibration method |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230526 |
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