Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing
InstructionCitrus is a globally important fruit tree whose microbiome plays a vital role in its growth, adaptability, and resistance to stress.MethodsWith the high throughput sequencing of 16S rRNA genes, this study focused on analyzing the bacterial community, especially in the leaf midribs, of hea...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1270929/full |
| _version_ | 1797628143817719808 |
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| author | Kaili Xia Kaili Xia Zengwei Feng Xianjiao Zhang Xianjiao Zhang Yang Zhou Honghui Zhu Qing Yao |
| author_facet | Kaili Xia Kaili Xia Zengwei Feng Xianjiao Zhang Xianjiao Zhang Yang Zhou Honghui Zhu Qing Yao |
| author_sort | Kaili Xia |
| collection | DOAJ |
| description | InstructionCitrus is a globally important fruit tree whose microbiome plays a vital role in its growth, adaptability, and resistance to stress.MethodsWith the high throughput sequencing of 16S rRNA genes, this study focused on analyzing the bacterial community, especially in the leaf midribs, of healthy and Huanglongbing (HLB)-infected plants.ResultsWe firstly identified the shared bacterial taxa in the midribs of both healthy and HLB-infected plants, and then analyzed their functions. Results showed that the shared bacterial taxa in midribs belonged to 62 genera, with approximately 1/3 of which modified in the infected samples. Furthermore, 366 metabolic pathways, 5851 proteins, and 1833 enzymes in the shared taxa were predicted. Among these, three metabolic pathways and one protein showed significant importance in HLB infection. With the random forest method, six genera were identified to be significantly important for HLB infection. Notably, four of these genera were also among the significantly different shared taxa. Further functional characterization of these four genera revealed that Pseudomonas and Erwinia likely contributed to plant defense against HLB, while Streptomyces might have implications for plant defense against HLB or the pathogenicity of Candidatus Liberibacter asiaticus (CLas).DisccusionOverall, our study highlights that the functions of the shared taxa in leaf midribs are distinguished between healthy and HLB-infected plants, and these microbiome-based findings can contribute to the management and protection of citrus crops against CLas. |
| first_indexed | 2024-03-11T10:34:17Z |
| format | Article |
| id | doaj.art-1de53bd08d0a4026be481f136780465c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T10:34:17Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1de53bd08d0a4026be481f136780465c2023-11-14T11:27:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12709291270929Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of HuanglongbingKaili Xia0Kaili Xia1Zengwei Feng2Xianjiao Zhang3Xianjiao Zhang4Yang Zhou5Honghui Zhu6Qing Yao7Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, ChinaInstructionCitrus is a globally important fruit tree whose microbiome plays a vital role in its growth, adaptability, and resistance to stress.MethodsWith the high throughput sequencing of 16S rRNA genes, this study focused on analyzing the bacterial community, especially in the leaf midribs, of healthy and Huanglongbing (HLB)-infected plants.ResultsWe firstly identified the shared bacterial taxa in the midribs of both healthy and HLB-infected plants, and then analyzed their functions. Results showed that the shared bacterial taxa in midribs belonged to 62 genera, with approximately 1/3 of which modified in the infected samples. Furthermore, 366 metabolic pathways, 5851 proteins, and 1833 enzymes in the shared taxa were predicted. Among these, three metabolic pathways and one protein showed significant importance in HLB infection. With the random forest method, six genera were identified to be significantly important for HLB infection. Notably, four of these genera were also among the significantly different shared taxa. Further functional characterization of these four genera revealed that Pseudomonas and Erwinia likely contributed to plant defense against HLB, while Streptomyces might have implications for plant defense against HLB or the pathogenicity of Candidatus Liberibacter asiaticus (CLas).DisccusionOverall, our study highlights that the functions of the shared taxa in leaf midribs are distinguished between healthy and HLB-infected plants, and these microbiome-based findings can contribute to the management and protection of citrus crops against CLas.https://www.frontiersin.org/articles/10.3389/fpls.2023.1270929/fullcitrusHLB -Huanglongbingleaf midrib bacterial communityshared bacterial taxarandom forestfunction prediction |
| spellingShingle | Kaili Xia Kaili Xia Zengwei Feng Xianjiao Zhang Xianjiao Zhang Yang Zhou Honghui Zhu Qing Yao Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing Frontiers in Plant Science citrus HLB -Huanglongbing leaf midrib bacterial community shared bacterial taxa random forest function prediction |
| title | Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing |
| title_full | Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing |
| title_fullStr | Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing |
| title_full_unstemmed | Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing |
| title_short | Potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of Huanglongbing |
| title_sort | potential functions of the shared bacterial taxa in the citrus leaf midribs determine the symptoms of huanglongbing |
| topic | citrus HLB -Huanglongbing leaf midrib bacterial community shared bacterial taxa random forest function prediction |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1270929/full |
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