Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells
<p>Abstract</p> <p>Background</p> <p><it>Enterobacter sakazakii </it>is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now l...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-06-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/6/58 |
| _version_ | 1811275439712239616 |
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| author | Pospischil Andreas Kim Kwang Wild Peter Borel Nicole Stephan Roger Mange Jean-Philippe Lehner Angelika |
| author_facet | Pospischil Andreas Kim Kwang Wild Peter Borel Nicole Stephan Roger Mange Jean-Philippe Lehner Angelika |
| author_sort | Pospischil Andreas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Enterobacter sakazakii </it>is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now little is known about the mechanisms of pathogenicity in <it>E. sakazakii</it>. A necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epithelial or endothelial tissue.</p> <p>This study examined for the first time the adherence ability of 50 <it>E. sakazakii </it>strains to the two epithelial cell lines HEp-2 and Caco-2, as well as the brain microvascular endothelial cell line HBMEC. Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence.</p> <p>Results</p> <p>Two distinctive adherence patterns, a diffuse adhesion and the formation of localized clusters of bacteria on the cell surface could be distinguished on all three cell lines. In some strains, a mixture of both patterns was observed. Adherence was maximal during late exponential phase, and increased with higher MOI. The adhesion capacity of <it>E. sakazakii </it>to HBMEC cells was affected by the addition of blood to the bacteria growth medium. Mannose, hemagglutination, trypsin digestion experiments and transmission electron microscopy suggested that the adhesion of <it>E. sakazakii </it>to the epithelial and endothelial cells is mainly non-fimbrial based.</p> <p>Conclusion</p> <p>Adherence experiments show heterogeneity within different <it>E. sakazakii </it>strains. In agreement with studies on <it>E. cloacae</it>, we found no relationship between the adhesive capacities in <it>E. sakazakii </it>and the eventual production of specific fimbriae. Further studies will have to be carried out in order to determine the adhesin(s) involved in the interaction of <it>E. sakazakii </it>with cells and to enhance knowledge of the pathogenesis of <it>E. sakazakii </it>infection.</p> |
| first_indexed | 2024-04-12T23:37:52Z |
| format | Article |
| id | doaj.art-1de7ab07c185427ba726da85d3e30ca4 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T23:37:52Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-1de7ab07c185427ba726da85d3e30ca42022-12-22T03:12:03ZengBMCBMC Microbiology1471-21802006-06-01615810.1186/1471-2180-6-58Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cellsPospischil AndreasKim KwangWild PeterBorel NicoleStephan RogerMange Jean-PhilippeLehner Angelika<p>Abstract</p> <p>Background</p> <p><it>Enterobacter sakazakii </it>is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now little is known about the mechanisms of pathogenicity in <it>E. sakazakii</it>. A necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epithelial or endothelial tissue.</p> <p>This study examined for the first time the adherence ability of 50 <it>E. sakazakii </it>strains to the two epithelial cell lines HEp-2 and Caco-2, as well as the brain microvascular endothelial cell line HBMEC. Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence.</p> <p>Results</p> <p>Two distinctive adherence patterns, a diffuse adhesion and the formation of localized clusters of bacteria on the cell surface could be distinguished on all three cell lines. In some strains, a mixture of both patterns was observed. Adherence was maximal during late exponential phase, and increased with higher MOI. The adhesion capacity of <it>E. sakazakii </it>to HBMEC cells was affected by the addition of blood to the bacteria growth medium. Mannose, hemagglutination, trypsin digestion experiments and transmission electron microscopy suggested that the adhesion of <it>E. sakazakii </it>to the epithelial and endothelial cells is mainly non-fimbrial based.</p> <p>Conclusion</p> <p>Adherence experiments show heterogeneity within different <it>E. sakazakii </it>strains. In agreement with studies on <it>E. cloacae</it>, we found no relationship between the adhesive capacities in <it>E. sakazakii </it>and the eventual production of specific fimbriae. Further studies will have to be carried out in order to determine the adhesin(s) involved in the interaction of <it>E. sakazakii </it>with cells and to enhance knowledge of the pathogenesis of <it>E. sakazakii </it>infection.</p>http://www.biomedcentral.com/1471-2180/6/58 |
| spellingShingle | Pospischil Andreas Kim Kwang Wild Peter Borel Nicole Stephan Roger Mange Jean-Philippe Lehner Angelika Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells BMC Microbiology |
| title | Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells |
| title_full | Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells |
| title_fullStr | Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells |
| title_full_unstemmed | Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells |
| title_short | Adhesive properties of <it>Enterobacter sakazakii </it>to human epithelial and brain microvascular endothelial cells |
| title_sort | adhesive properties of it enterobacter sakazakii it to human epithelial and brain microvascular endothelial cells |
| url | http://www.biomedcentral.com/1471-2180/6/58 |
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