Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities?
Background: The aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2015-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503295C.pdf |
| _version_ | 1811293159320190976 |
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| author | Cvetković Tatjana Veličković-Radovanović Radmila Stojanović Dijana Stefanović Nikola Ignjatović Aleksandra Stojanović Ivana Sladojević Nikola Pavlović Dušica |
| author_facet | Cvetković Tatjana Veličković-Radovanović Radmila Stojanović Dijana Stefanović Nikola Ignjatović Aleksandra Stojanović Ivana Sladojević Nikola Pavlović Dušica |
| author_sort | Cvetković Tatjana |
| collection | DOAJ |
| description | Background: The aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among these parameters and to evaluate potential differences in all the biomarkers with regard to the immunosuppression protocol. Methods: We enrolled 57 renal transplant recipients and 31 controls who were age and sex matched with the renal transplant recipients. All of the patients included in this study had post-renal transplant surgery at least 12 months earlier and were on standard immunosuppressive therapy. In this study, we determined thiobarbituric acid-reactive substances in plasma and red blood cells and advanced oxidation protein products, nitrosative stress parameters (asymmetric and symmetric dimethylarginine - ADMA and SDMA), and antioxidative parameters (total SH groups and catalase activity). Results: The results of our study demonstrated that the levels of oxidative and nitrosative stress were significantly increased compared to the healthy population (p < 0.01 except for plasma catalase activity p < 0.05). Correlation analysis showed significant positive correlations between: ADM A and SDMA (p < 0.01); ADM A and nitrates (p< 0.05); SDMA and nitrates (p < 0.05); between OS parameters in the experimental group; AOPP and SH groups (p < 0.05) and TBARS in plasma and SH groups (p < 0.01), SDMA and AOPP (p< 0.05); SDMA and TBARS in plasma (p < 0.05); SDMA and SH groups (p < 0.01); nitrates and SH groups (p < 0.05). Conclusion: There was no significant difference in oxidative and nitrosative stress parameters with respect to the immunosuppressive protocol. |
| first_indexed | 2024-04-13T04:56:50Z |
| format | Article |
| id | doaj.art-1dee74a8ad0242d8b07f9fd9a258a206 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-04-13T04:56:50Z |
| publishDate | 2015-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-1dee74a8ad0242d8b07f9fd9a258a2062022-12-22T03:01:27ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662015-01-013432953031452-82581503295COxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities?Cvetković Tatjana0Veličković-Radovanović Radmila1https://orcid.org/0000-0001-9055-3378Stojanović Dijana2Stefanović Nikola3https://orcid.org/0000-0003-2599-7508Ignjatović Aleksandra4https://orcid.org/0000-0002-2689-8088Stojanović Ivana5Sladojević Nikola6Pavlović Dušica7University of Niš, Medical Faculty, Institute of Biochemistry + Clinical Centre Niš, Clinic for Nephrology, Dialysis and TransplantationClinical Centre Niš, Clinic for Nephrology, Dialysis and Transplantation + University of Niš, Medical Faculty, Pharmacy DepartmentUniversity of Niš, Medical Faculty, Institute of PathophysiologyUniversity of Niš, Medical Faculty, Pharmacy DepartmentPublic Health Institute, NišUniversity of Niš, Medical Faculty, Institute of BiochemistryMedical Center Dr. Ann Arbor, Michigan, USAUniversity of Niš, Medical Faculty, Institute of BiochemistryBackground: The aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among these parameters and to evaluate potential differences in all the biomarkers with regard to the immunosuppression protocol. Methods: We enrolled 57 renal transplant recipients and 31 controls who were age and sex matched with the renal transplant recipients. All of the patients included in this study had post-renal transplant surgery at least 12 months earlier and were on standard immunosuppressive therapy. In this study, we determined thiobarbituric acid-reactive substances in plasma and red blood cells and advanced oxidation protein products, nitrosative stress parameters (asymmetric and symmetric dimethylarginine - ADMA and SDMA), and antioxidative parameters (total SH groups and catalase activity). Results: The results of our study demonstrated that the levels of oxidative and nitrosative stress were significantly increased compared to the healthy population (p < 0.01 except for plasma catalase activity p < 0.05). Correlation analysis showed significant positive correlations between: ADM A and SDMA (p < 0.01); ADM A and nitrates (p< 0.05); SDMA and nitrates (p < 0.05); between OS parameters in the experimental group; AOPP and SH groups (p < 0.05) and TBARS in plasma and SH groups (p < 0.01), SDMA and AOPP (p< 0.05); SDMA and TBARS in plasma (p < 0.05); SDMA and SH groups (p < 0.01); nitrates and SH groups (p < 0.05). Conclusion: There was no significant difference in oxidative and nitrosative stress parameters with respect to the immunosuppressive protocol.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503295C.pdfadmasdmaoxidative stressnitrosative stressrenal transplantation |
| spellingShingle | Cvetković Tatjana Veličković-Radovanović Radmila Stojanović Dijana Stefanović Nikola Ignjatović Aleksandra Stojanović Ivana Sladojević Nikola Pavlović Dušica Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? Journal of Medical Biochemistry adma sdma oxidative stress nitrosative stress renal transplantation |
| title | Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? |
| title_full | Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? |
| title_fullStr | Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? |
| title_full_unstemmed | Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? |
| title_short | Oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol: Differences or similarities? |
| title_sort | oxidative and nitrosative stress in stable renal transplant recipients with respect to the immunosuppression protocol differences or similarities |
| topic | adma sdma oxidative stress nitrosative stress renal transplantation |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503295C.pdf |
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