LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study
A specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100538&lng=en&tlng=en |
| _version_ | 1819024019019530240 |
|---|---|
| author | Márcia Camponogara Fontana João Víctor Laureano Betielli Forgearini Paula dos Santos Chaves Bibiana Verlindo de Araujo Ruy Carlos Ruver Beck |
| author_facet | Márcia Camponogara Fontana João Víctor Laureano Betielli Forgearini Paula dos Santos Chaves Bibiana Verlindo de Araujo Ruy Carlos Ruver Beck |
| author_sort | Márcia Camponogara Fontana |
| collection | DOAJ |
| description | A specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M (pH 4.0) as mobile phase at a flow rate of 1 mL.min-1 and UV detection at 287 nm. Retention times of raloxifene and internal standard (dexamethasone) were approximately 11 min and 14 min, respectively. Linearity was checked for a concentration range between 25 ng.mL-1 and 1000 ng.mL-1. Intra- and inter-day precision had relative standard deviation lower than 10% and 15%, respectively. Recovery from plasma was higher than 90%. Accuracy values were 98.21%, 99.70%, and 102.70% for lower, medium, and upper limits of quantification, respectively. Limit of quantification was 25 ng.mL-1. Drug stability was analyzed at room temperature using plasma kept in a freezer at -80 °C for 45 days after processing for 6 h and three freeze-thaw cycles. The advantages of the method developed include stability under different conditions and low limit of quantification. Its applicability was confirmed by the analysis of raloxifene levels in plasma samples in a designed pharmacokinetic study in rats after intravenous administration (5 mg.kg-1). |
| first_indexed | 2024-12-21T04:48:07Z |
| format | Article |
| id | doaj.art-1defd3c95d474127a3e132749831146a |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-21T04:48:07Z |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-1defd3c95d474127a3e132749831146a2022-12-21T19:15:31ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97905510.1590/s2175-97902019000118052S1984-82502019000100538LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic studyMárcia Camponogara FontanaJoão Víctor LaureanoBetielli ForgeariniPaula dos Santos ChavesBibiana Verlindo de AraujoRuy Carlos Ruver BeckA specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M (pH 4.0) as mobile phase at a flow rate of 1 mL.min-1 and UV detection at 287 nm. Retention times of raloxifene and internal standard (dexamethasone) were approximately 11 min and 14 min, respectively. Linearity was checked for a concentration range between 25 ng.mL-1 and 1000 ng.mL-1. Intra- and inter-day precision had relative standard deviation lower than 10% and 15%, respectively. Recovery from plasma was higher than 90%. Accuracy values were 98.21%, 99.70%, and 102.70% for lower, medium, and upper limits of quantification, respectively. Limit of quantification was 25 ng.mL-1. Drug stability was analyzed at room temperature using plasma kept in a freezer at -80 °C for 45 days after processing for 6 h and three freeze-thaw cycles. The advantages of the method developed include stability under different conditions and low limit of quantification. Its applicability was confirmed by the analysis of raloxifene levels in plasma samples in a designed pharmacokinetic study in rats after intravenous administration (5 mg.kg-1).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100538&lng=en&tlng=enRaloxifene/pharmacokineticLiquid chromatography.Plasma.Bioavailability. |
| spellingShingle | Márcia Camponogara Fontana João Víctor Laureano Betielli Forgearini Paula dos Santos Chaves Bibiana Verlindo de Araujo Ruy Carlos Ruver Beck LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study Brazilian Journal of Pharmaceutical Sciences Raloxifene/pharmacokinetic Liquid chromatography. Plasma. Bioavailability. |
| title | LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| title_full | LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| title_fullStr | LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| title_full_unstemmed | LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| title_short | LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| title_sort | lc uv method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study |
| topic | Raloxifene/pharmacokinetic Liquid chromatography. Plasma. Bioavailability. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100538&lng=en&tlng=en |
| work_keys_str_mv | AT marciacamponogarafontana lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy AT joaovictorlaureano lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy AT betielliforgearini lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy AT pauladossantoschaves lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy AT bibianaverlindodearaujo lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy AT ruycarlosruverbeck lcuvmethodtoassayraloxifenehydrochlorideinratplasmaanditsapplicationtoapharmacokineticstudy |