Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>)
<i>Seseli</i> L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two <i>Seseli</i> species, <i>S. gummiferum</i> and <i>S. transcaucasi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1510 |
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| author | Gokhan Zengin Dejan Stojković Mohamad Fawzi Mahomoodally Bibi Sharmeen Jugreet Mehmet Yavuz Paksoy Marija Ivanov Uroš Gašić Monica Gallo Domenico Montesano |
| author_facet | Gokhan Zengin Dejan Stojković Mohamad Fawzi Mahomoodally Bibi Sharmeen Jugreet Mehmet Yavuz Paksoy Marija Ivanov Uroš Gašić Monica Gallo Domenico Montesano |
| author_sort | Gokhan Zengin |
| collection | DOAJ |
| description | <i>Seseli</i> L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two <i>Seseli</i> species, <i>S. gummiferum</i> and <i>S. transcaucasicum</i> were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of <i>S. transcaucasicum</i> (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic <i>S. transcaucasicum</i> extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of <i>S. gummiferum</i> displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both <i>Seseli</i> species showed lower cytotoxicity towards HaCaT cells (IC<sub>50</sub>: >500 µg/mL), compared to the water extracts (IC<sub>50</sub>: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against <i>Staphylococcus lugdunensis</i> (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). <i>S. gummiferum</i> methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of <i>S. lugdunensis</i> to HaCaT cells as well. Taken together, this study demonstrated the two <i>Seseli</i> species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses. |
| first_indexed | 2024-03-10T06:45:58Z |
| format | Article |
| id | doaj.art-1df0a5a85ad44b01b5df66b103dd816c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:58Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1df0a5a85ad44b01b5df66b103dd816c2023-11-22T17:15:04ZengMDPI AGAntioxidants2076-39212021-09-011010151010.3390/antiox10101510Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>)Gokhan Zengin0Dejan Stojković1Mohamad Fawzi Mahomoodally2Bibi Sharmeen Jugreet3Mehmet Yavuz Paksoy4Marija Ivanov5Uroš Gašić6Monica Gallo7Domenico Montesano8Department of Biology, Science Faculty, Selcuk University, 42130 Konya, TurkeyDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, MauritiusDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, MauritiusDepartment of Medical Services and Techniques, Medical Documentation and Secretaryship Programme, Tunceli Vocational School, Munzur University, 62000 Tunceli, TurkeyDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy<i>Seseli</i> L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two <i>Seseli</i> species, <i>S. gummiferum</i> and <i>S. transcaucasicum</i> were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of <i>S. transcaucasicum</i> (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic <i>S. transcaucasicum</i> extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of <i>S. gummiferum</i> displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both <i>Seseli</i> species showed lower cytotoxicity towards HaCaT cells (IC<sub>50</sub>: >500 µg/mL), compared to the water extracts (IC<sub>50</sub>: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against <i>Staphylococcus lugdunensis</i> (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). <i>S. gummiferum</i> methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of <i>S. lugdunensis</i> to HaCaT cells as well. Taken together, this study demonstrated the two <i>Seseli</i> species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.https://www.mdpi.com/2076-3921/10/10/1510<i>Seseli</i>polyphenolicantioxidantenzyme inhibitorsantibacterialwound healing |
| spellingShingle | Gokhan Zengin Dejan Stojković Mohamad Fawzi Mahomoodally Bibi Sharmeen Jugreet Mehmet Yavuz Paksoy Marija Ivanov Uroš Gašić Monica Gallo Domenico Montesano Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) Antioxidants <i>Seseli</i> polyphenolic antioxidant enzyme inhibitors antibacterial wound healing |
| title | Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) |
| title_full | Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) |
| title_fullStr | Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) |
| title_full_unstemmed | Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) |
| title_short | Comprehensive Biological and Chemical Evaluation of Two <i>Seseli</i> Species (<i>S. gummiferum</i> and <i>S. transcaucasicum</i>) |
| title_sort | comprehensive biological and chemical evaluation of two i seseli i species i s gummiferum i and i s transcaucasicum i |
| topic | <i>Seseli</i> polyphenolic antioxidant enzyme inhibitors antibacterial wound healing |
| url | https://www.mdpi.com/2076-3921/10/10/1510 |
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